FastQCFastQC Report
Thu 26 May 2016
SRR1545020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545020_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences549937
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT21870.3976819162922298No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT17710.3220368878617005No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT17130.31149022524398245Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.18674866393786924No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT7290.13256063876407662No Hit
ACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC7240.13165144371082507No Hit
GCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC6020.10946708441148714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.0182703E-746.00000424
CTGTCGG351.0182703E-746.0000041
CAACGCA1400.046.00000416
ATTCCGG351.0182703E-746.0000041
GTAGCGG1400.046.0000041
CGAATAT351.0182703E-746.00000414
GCGATAA351.0182703E-746.0000048
CGCACTT1150.046.00000434
GCTACGA351.0182703E-746.00000410
CCGCACT1150.046.00000433
CGGGTCA253.4148146E-546.05
CGTGGGA253.4148146E-546.03
CGGGTAG206.3085684E-446.05
AATCCGG501.6370905E-1146.01
GGTGTAT206.3085684E-446.08
CGACGGT253.4148146E-546.027
ACCGCTT206.3085684E-446.021
ATTACGG253.4148146E-546.01
CGGCACC206.3085684E-446.027
GGCAACT253.4148146E-546.07
Produced by FastQC (version 0.11.5)