FastQCFastQC Report
Thu 26 May 2016
SRR1545019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545019_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences348178
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT19760.5675258057660162No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT13870.39835945981653065Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT13000.373372240635537No Hit
TGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTTG8170.23465009276864135Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.1953024027939732No Hit
ACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC5280.1516465715812027No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT4990.1433174985208715No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4650.13355237838117284No Hit
GCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC4590.1318291218859319No Hit
AGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC4100.1177558605081309No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC3950.11344771927002854No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG3710.10655469328906479No Hit
ACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT3530.10138492380334196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG301.8576393E-646.01
GTCGAGG453.092282E-1046.01
GTCGAAT253.4122164E-546.029
CAACGCA850.046.016
TGGACTA206.305363E-446.013
GGCACCC206.305363E-446.07
GGCAAAC253.4122164E-546.07
TCTTAAG551.8189894E-1246.011
TGTATGG551.8189894E-1246.01
GTAGGGT600.046.03
CTAAGCG206.305363E-446.017
TAACGGA206.305363E-446.028
TATGCAA206.305363E-446.09
ACCTCGG253.4122164E-546.01
ATATCGG253.4122164E-546.01
ACAGGGT405.5915734E-946.03
GTTAACG206.305363E-446.026
ATATAGG253.4122164E-546.01
CTAAACG253.4122164E-546.045
CTACGAA206.305363E-446.011
Produced by FastQC (version 0.11.5)