##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545019_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 348178 Sequences flagged as poor quality 0 Sequence length 52 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.949000224023344 34.0 33.0 34.0 31.0 34.0 2 33.28061795977919 34.0 34.0 34.0 31.0 34.0 3 33.39169620136826 34.0 34.0 34.0 31.0 34.0 4 36.61870077948635 37.0 37.0 37.0 35.0 37.0 5 36.61400203344267 37.0 37.0 37.0 35.0 37.0 6 36.447004118583024 37.0 37.0 37.0 35.0 37.0 7 36.284219565854244 37.0 37.0 37.0 35.0 37.0 8 36.421502794547614 37.0 37.0 37.0 35.0 37.0 9 38.286428206262315 39.0 39.0 39.0 37.0 39.0 10 38.1276272481317 39.0 39.0 39.0 35.0 39.0 11 37.31174571627156 39.0 35.0 39.0 35.0 39.0 12 37.26023183544049 39.0 35.0 39.0 35.0 39.0 13 37.23165162646692 39.0 35.0 39.0 35.0 39.0 14 38.394036958107634 40.0 35.0 41.0 35.0 41.0 15 38.46333484596959 41.0 35.0 41.0 35.0 41.0 16 38.51274348178231 41.0 35.0 41.0 35.0 41.0 17 38.6150991734113 41.0 36.0 41.0 35.0 41.0 18 38.619858233432325 40.0 36.0 41.0 35.0 41.0 19 38.5429090867315 40.0 35.0 41.0 35.0 41.0 20 38.459853867849205 40.0 35.0 41.0 35.0 41.0 21 38.41861633991809 40.0 35.0 41.0 35.0 41.0 22 38.40467232277743 40.0 35.0 41.0 35.0 41.0 23 38.383039709573836 40.0 35.0 41.0 35.0 41.0 24 38.363365864586505 40.0 35.0 41.0 35.0 41.0 25 38.32687016411146 40.0 35.0 41.0 35.0 41.0 26 38.27434243404236 40.0 35.0 41.0 35.0 41.0 27 38.362099271062505 40.0 35.0 41.0 35.0 41.0 28 38.388545514076135 40.0 35.0 41.0 35.0 41.0 29 38.39390771387049 40.0 35.0 41.0 35.0 41.0 30 38.311981227992575 40.0 35.0 41.0 35.0 41.0 31 38.24541182958142 40.0 35.0 41.0 35.0 41.0 32 38.18726915543199 40.0 35.0 41.0 35.0 41.0 33 38.125157247155194 40.0 35.0 41.0 35.0 41.0 34 38.006577095623506 40.0 35.0 41.0 35.0 41.0 35 37.95009736399198 40.0 35.0 41.0 35.0 41.0 36 37.91577296670094 40.0 35.0 41.0 35.0 41.0 37 37.84476043862622 40.0 35.0 41.0 35.0 41.0 38 37.83898752936716 40.0 35.0 41.0 35.0 41.0 39 37.75797436943173 40.0 35.0 41.0 35.0 41.0 40 37.72352647209186 40.0 35.0 41.0 35.0 41.0 41 37.667262147522244 40.0 35.0 41.0 35.0 41.0 42 37.621440757313785 40.0 35.0 41.0 35.0 41.0 43 37.562493896799914 39.0 35.0 41.0 35.0 41.0 44 37.37074140238614 39.0 35.0 41.0 35.0 41.0 45 37.40495378800499 39.0 35.0 41.0 35.0 41.0 46 37.3910183871468 39.0 35.0 41.0 35.0 41.0 47 37.332970492104614 39.0 35.0 41.0 35.0 41.0 48 37.31195537914515 39.0 35.0 41.0 35.0 41.0 49 37.24189064214281 39.0 35.0 41.0 35.0 41.0 50 37.18083566451642 39.0 35.0 41.0 35.0 41.0 51 37.11977781479588 38.0 35.0 41.0 34.0 41.0 52 36.68701928324018 37.0 35.0 40.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 1.0 15 1.0 16 5.0 17 10.0 18 21.0 19 39.0 20 65.0 21 147.0 22 183.0 23 337.0 24 575.0 25 1026.0 26 1492.0 27 1704.0 28 1563.0 29 1548.0 30 1718.0 31 2097.0 32 3026.0 33 4961.0 34 17199.0 35 84062.0 36 9534.0 37 15481.0 38 30191.0 39 169588.0 40 1603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.15015882680698 22.450585619998968 24.14598280190018 22.253272751293878 2 26.516896529935835 24.042300202769848 27.217974714083027 22.222828553211286 3 25.010483143679384 25.47662402564206 26.502536059142162 23.010356771536397 4 25.82960439775058 27.147895616609897 23.385452268667176 23.637047716972354 5 23.041375388450735 35.349447696293275 21.22219094830805 20.38698596694794 6 86.32050273136154 2.425770726467497 8.072595051956183 3.181131490214775 7 86.21940501697406 1.8619786431078356 5.928289553044707 5.990326786873381 8 75.72046481971864 4.311013332261085 12.711888746560668 7.256633101459599 9 52.295377651660935 17.037836968447174 15.347896765447558 15.318888614444337 10 43.6762230812975 17.554813917019455 26.516322111104092 12.252640890578958 11 41.12293137418217 16.296549466077696 24.246218888040026 18.33430027170011 12 19.318854149314433 35.23427672052801 28.526213603386775 16.92065552677079 13 13.284871531228278 37.70600095353526 29.524553532963026 19.484573982273435 14 12.025171033207153 21.62055040812458 48.72335414644234 17.63092441222593 15 13.629235620860594 16.346811113855555 49.52524283556112 20.498710429722728 16 13.735503104733784 20.315758031811313 29.050945206187638 36.89779365726726 17 17.74781864448644 22.204159941179512 36.19815152019944 23.84986989413461 18 24.084232777487376 27.0134816099811 28.006651770071628 20.89563384245989 19 26.13864172923045 22.50199610544032 25.527747301667535 25.831614863661688 20 17.634370925216412 32.56725008472678 27.165415390978175 22.632963599078632 21 18.520124763770255 24.17010839283355 25.708689233667837 31.601077609728357 22 15.78675275290225 31.776849772242933 23.44088368593076 28.995513788924054 23 16.4904158217923 22.704191534215255 38.71008507142898 22.095307572563456 24 17.644710464187856 22.398026296894116 35.35519188461074 24.602071354307277 25 13.930231088696013 31.023786683822642 30.721929587739606 24.32405263974174 26 17.26559403523485 30.44333645434232 26.96724089402547 25.32382861639736 27 18.176047883553814 28.308221656738798 36.20188524260579 17.313845217101598 28 15.927772576096135 25.232783231565463 32.60027916755222 26.23916502478617 29 19.010104027250428 30.629160946412465 27.272257293683115 23.088477732653985 30 26.71219893272981 27.559466709556606 21.36378519033368 24.364549167379902 31 27.64965046614088 27.21079447868619 23.877154788642592 21.26240026653034 32 25.44416936164835 31.51577641321393 20.91458966390754 22.125464561230178 33 16.774465934091186 32.50808494505684 26.767917559409266 23.94953156144271 34 19.610658915841896 25.742867154156784 29.863460643693745 24.78301328630758 35 28.595431072612282 27.417872467530973 25.1500669197939 18.83662954006284 36 24.433479427189543 30.079729333846483 23.969636220553856 21.517155018410122 37 19.309089029174732 33.72010868004296 23.942351326045873 23.02845096473643 38 21.27446306199702 29.4099569760295 23.403833671283078 25.911746290690395 39 26.979878108323902 25.461401926600765 23.682426804680365 23.876293160394972 40 17.728001194791172 22.23201925451924 28.28237280931018 31.75760674137941 41 21.596137607775333 26.568019805961317 23.08876494206986 28.747077644193485 42 22.456904227148183 23.292970836755913 27.27685264433709 26.973272291758814 43 24.237602605563822 27.311604983657784 27.629258597613866 20.821533813164532 44 17.30436730637777 31.986225436414706 25.925532342652318 24.7838749145552 45 21.812119088512198 29.47457909460104 25.789395079528287 22.923906737358475 46 23.940053650718884 23.15683357363188 26.933062973536526 25.97004980211271 47 22.655365933516762 20.946182699653626 31.11799137222915 25.280459994600463 48 21.784834194004215 18.196152542664958 28.720367168517253 31.29864609481357 49 20.84766987000902 24.29935262997662 30.166753786856148 24.686223713158213 50 19.79188805725807 26.4198197473706 27.06029674476848 26.727995450602855 51 19.356765792209732 20.921482689888503 31.458908948870977 28.262842569030784 52 22.896621842850497 19.13676337965064 33.80713313305264 24.15948164444623 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 102.0 2 157.0 3 699.0 4 1241.0 5 862.0 6 483.0 7 537.5 8 592.0 9 635.0 10 678.0 11 662.5 12 647.0 13 631.5 14 571.0 15 526.0 16 503.5 17 481.0 18 470.5 19 460.0 20 499.5 21 539.0 22 512.0 23 485.0 24 562.0 25 639.0 26 760.0 27 881.0 28 1067.0 29 1253.0 30 1478.0 31 1703.0 32 1901.0 33 2099.0 34 2634.5 35 3170.0 36 4146.0 37 5122.0 38 5724.0 39 7781.5 40 9237.0 41 15286.0 42 21335.0 43 30325.0 44 39315.0 45 46461.0 46 53607.0 47 52661.5 48 51716.0 49 44666.0 50 37616.0 51 31666.5 52 25717.0 53 22222.5 54 18728.0 55 16016.0 56 13304.0 57 12087.0 58 10870.0 59 10799.5 60 10729.0 61 9896.0 62 9063.0 63 8205.5 64 5900.5 65 4453.0 66 3388.0 67 2323.0 68 1946.0 69 1569.0 70 1375.5 71 1182.0 72 1035.0 73 888.0 74 687.0 75 486.0 76 369.5 77 253.0 78 198.0 79 143.0 80 107.0 81 71.0 82 51.5 83 32.0 84 19.0 85 6.0 86 15.5 87 25.0 88 21.0 89 8.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 348178.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.24757583864304 #Duplication Level Percentage of deduplicated Percentage of total 1 69.20660511993134 20.241254317802138 2 11.970595385772981 7.002217927582115 3 4.242886257969318 3.7228241281408216 4 2.033777699068773 2.3793226996981955 5 1.2132415121366145 1.77421865684028 6 0.9424092026975566 1.6537910776159146 7 0.7485306530819289 1.5324894930493904 8 0.593627775726338 1.3889738712384845 9 0.5566509610637362 1.4652622079428645 >10 8.135898516700198 48.00202500499279 >50 0.2808239139162557 5.372043510568743 >100 0.06895676178014819 3.5107249907743174 >500 0.002998120077397748 0.5918922742750806 >1k 0.002998120077397748 1.3629598394788647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT 1976 0.5675258057660162 No Hit CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT 1387 0.39835945981653065 Illumina Single End Adapter 1 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT 1300 0.373372240635537 No Hit TGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTTG 817 0.23465009276864135 Illumina Single End Adapter 1 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 680 0.1953024027939732 No Hit ACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC 528 0.1516465715812027 No Hit TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT 499 0.1433174985208715 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA 465 0.13355237838117284 No Hit GCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC 459 0.1318291218859319 No Hit AGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC 410 0.1177558605081309 No Hit CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC 395 0.11344771927002854 No Hit ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG 371 0.10655469328906479 No Hit ACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT 353 0.10138492380334196 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.40927341761972325 0.0 2 0.0 0.0 0.0 1.6207227337741041 0.0 3 0.0 0.0 0.0 2.573683575642344 0.0 4 0.0 0.0 0.0 3.1567186898655284 0.0 5 0.0 0.0 0.0 6.355944373280334 0.0 6 0.0 0.0 0.0 6.993836485935355 0.0 7 0.0 0.0 0.0 10.312541286353532 0.0 8 0.0 0.0 0.0 13.651350745882853 0.0 9 0.0 0.0 0.0 17.115383510733015 0.0 10 0.0 0.0 0.0 18.456938692278086 0.0 11 0.0 0.0 0.0 19.736456639994486 0.0 12 0.0 0.0 0.0 20.89391058596465 0.0 13 0.0 0.0 0.0 21.29542934935579 0.0 14 0.0 0.0 0.0 21.588095744130875 0.0 15 0.0 0.0 0.0 22.662258959497727 0.0 16 0.0 0.0 0.0 23.764855907036058 0.0 17 0.0 0.0 0.0 25.411427488238775 0.0 18 0.0 0.0 0.0 26.051617276220785 0.0 19 0.0 0.0 0.0 26.875333880945952 0.0 20 2.8720941587349E-4 0.0 0.0 27.811349367277657 0.0 21 2.8720941587349E-4 0.0 0.0 28.549477566072525 0.0 22 2.8720941587349E-4 0.0 0.0 29.488077937147093 0.0 23 2.8720941587349E-4 0.0 0.0 30.1044293436116 0.0 24 2.8720941587349E-4 0.0 0.0 30.62198071101563 0.0 25 2.8720941587349E-4 0.0 0.0 31.13637277484505 0.0 26 2.8720941587349E-4 0.0 0.0 31.643010184445888 0.0 27 2.8720941587349E-4 0.0 0.0 32.13701037974829 0.0 28 2.8720941587349E-4 0.0 0.0 32.58735474383792 0.0 29 2.8720941587349E-4 0.0 0.0 33.21950266817547 0.0 30 2.8720941587349E-4 0.0 0.0 33.845331985363806 0.0 31 2.8720941587349E-4 0.0 0.0 34.319227521555064 0.0 32 2.8720941587349E-4 0.0 0.0 34.792835848330455 0.0 33 2.8720941587349E-4 0.0 0.0 35.24116974650897 0.0 34 2.8720941587349E-4 0.0 0.0 35.89284791112592 0.0 35 2.8720941587349E-4 0.0 0.0 36.360999258999705 0.0 36 2.8720941587349E-4 0.0 0.0 36.8713703910069 0.0 37 2.8720941587349E-4 0.0 0.0 37.298450792410776 0.0 38 2.8720941587349E-4 0.0 0.0 37.73730677986547 0.0 39 2.8720941587349E-4 0.0 0.0 38.230445346920256 0.0 40 2.8720941587349E-4 0.0 0.0 38.73794438476871 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCGG 30 1.8576393E-6 46.0 1 GTCGAGG 45 3.092282E-10 46.0 1 GTCGAAT 25 3.4122164E-5 46.0 29 CAACGCA 85 0.0 46.0 16 TGGACTA 20 6.305363E-4 46.0 13 GGCACCC 20 6.305363E-4 46.0 7 GGCAAAC 25 3.4122164E-5 46.0 7 TCTTAAG 55 1.8189894E-12 46.0 11 TGTATGG 55 1.8189894E-12 46.0 1 GTAGGGT 60 0.0 46.0 3 CTAAGCG 20 6.305363E-4 46.0 17 TAACGGA 20 6.305363E-4 46.0 28 TATGCAA 20 6.305363E-4 46.0 9 ACCTCGG 25 3.4122164E-5 46.0 1 ATATCGG 25 3.4122164E-5 46.0 1 ACAGGGT 40 5.5915734E-9 46.0 3 GTTAACG 20 6.305363E-4 46.0 26 ATATAGG 25 3.4122164E-5 46.0 1 CTAAACG 25 3.4122164E-5 46.0 45 CTACGAA 20 6.305363E-4 46.0 11 >>END_MODULE