FastQCFastQC Report
Thu 26 May 2016
SRR1545018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545018_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1087217
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC60640.5577543397500223No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG43560.40065598679932346No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT39470.36303700181288556TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38540.354483051681495No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT33880.31162132306614043TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT32450.2984684750146475Illumina Paired End PCR Primer 2 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC28320.26048157819460144No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.21522842266079356No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC20110.18496767434651962No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA16890.15535077174105996No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT13610.12518200138518806No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT13200.12141090509070407TruSeq Adapter, Index 22 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC12830.11800772062982826No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA11610.10678640970477835No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11330.104211026869521No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG206.3113024E-446.032
CGTATAC253.4170334E-546.016
GCGAACC206.3113024E-446.025
TAGCCGA206.3113024E-446.034
ATCGTTA253.4170334E-546.020
GTTCACG206.3113024E-446.017
TTACGCT253.4170334E-546.024
ACGTAGG600.046.01
TAGGCGA501.6370905E-1146.019
AGTAGCG301.86131E-646.032
CGACTAT206.3113024E-446.040
CGTCAAA206.3113024E-446.027
TAATCCG206.3113024E-446.025
ATAGCGG2100.044.9047621
CGTTTTT18950.043.5725631
TCACGAC1800.043.44444324
CCCGCGA800.043.12528
AATACGG650.042.461541
CGCATCG601.8189894E-1242.16666820
CTAGCGG3500.042.0571441
Produced by FastQC (version 0.11.5)