##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545018_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1087217
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88880508674901	34.0	33.0	34.0	31.0	34.0
2	33.196722457430305	34.0	33.0	34.0	31.0	34.0
3	33.26200749252449	34.0	34.0	34.0	31.0	34.0
4	36.541142200683026	37.0	37.0	37.0	35.0	37.0
5	36.49367513569048	37.0	37.0	37.0	35.0	37.0
6	36.61109879628446	37.0	37.0	37.0	35.0	37.0
7	36.49929039005093	37.0	37.0	37.0	35.0	37.0
8	36.52482623064209	37.0	37.0	37.0	35.0	37.0
9	38.301949840740164	39.0	39.0	39.0	37.0	39.0
10	38.116209551543065	39.0	39.0	39.0	35.0	39.0
11	37.49261554960969	39.0	35.0	39.0	35.0	39.0
12	37.39371992895622	39.0	35.0	39.0	35.0	39.0
13	37.30071733609758	39.0	35.0	39.0	35.0	39.0
14	38.674834002779576	41.0	35.0	41.0	35.0	41.0
15	38.757731897128174	41.0	35.0	41.0	35.0	41.0
16	38.77035587191885	41.0	35.0	41.0	35.0	41.0
17	38.80720132227513	41.0	36.0	41.0	35.0	41.0
18	38.79568844122195	41.0	36.0	41.0	35.0	41.0
19	38.717470385396844	41.0	36.0	41.0	35.0	41.0
20	38.65565015999566	41.0	35.0	41.0	35.0	41.0
21	38.62039316898098	41.0	35.0	41.0	35.0	41.0
22	38.56866108605734	40.0	35.0	41.0	35.0	41.0
23	38.57448605016294	40.0	35.0	41.0	35.0	41.0
24	38.50955788954735	40.0	35.0	41.0	35.0	41.0
25	38.44468767504555	40.0	35.0	41.0	35.0	41.0
26	38.386929196287404	40.0	35.0	41.0	35.0	41.0
27	38.34708802382597	40.0	35.0	41.0	35.0	41.0
28	38.37990483960424	40.0	35.0	41.0	35.0	41.0
29	38.3625182461275	40.0	36.0	41.0	35.0	41.0
30	38.28652145799781	40.0	35.0	41.0	35.0	41.0
31	38.2380159618549	40.0	35.0	41.0	35.0	41.0
32	38.12891446693715	40.0	35.0	41.0	35.0	41.0
33	38.05603573159728	40.0	35.0	41.0	35.0	41.0
34	37.90312145597429	40.0	35.0	41.0	35.0	41.0
35	37.85454237746467	40.0	35.0	41.0	35.0	41.0
36	37.78902831725405	40.0	35.0	41.0	35.0	41.0
37	37.75495048366609	40.0	35.0	41.0	35.0	41.0
38	37.67043837614754	40.0	35.0	41.0	35.0	41.0
39	37.605968265764794	40.0	35.0	41.0	34.0	41.0
40	37.54421978317116	40.0	35.0	41.0	34.0	41.0
41	37.511345941058686	40.0	35.0	41.0	34.0	41.0
42	37.423721299427804	39.0	35.0	41.0	34.0	41.0
43	37.34001123970652	39.0	35.0	41.0	34.0	41.0
44	37.24872403577207	39.0	35.0	41.0	34.0	41.0
45	37.1815497734123	39.0	35.0	41.0	34.0	41.0
46	37.15691531681348	39.0	35.0	41.0	34.0	41.0
47	37.08836506419602	39.0	35.0	41.0	34.0	41.0
48	37.07311419891337	38.0	35.0	41.0	34.0	41.0
49	36.994908100222865	38.0	35.0	41.0	34.0	41.0
50	36.902097741297275	38.0	35.0	41.0	34.0	41.0
51	36.80546661797967	37.0	35.0	41.0	34.0	41.0
52	36.56077857502228	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	16.0
14	12.0
15	16.0
16	25.0
17	62.0
18	99.0
19	189.0
20	290.0
21	514.0
22	764.0
23	1235.0
24	2157.0
25	3464.0
26	4847.0
27	5930.0
28	6110.0
29	6827.0
30	7983.0
31	10044.0
32	12740.0
33	18338.0
34	62270.0
35	190719.0
36	42285.0
37	66754.0
38	121865.0
39	517854.0
40	3801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.94868089810958	21.025333489082676	23.773910820011093	20.252074792796655
2	28.20163775952731	24.05444359313734	26.00078917088309	21.743129476452264
3	29.04010882832038	24.183672624692218	25.436412418128118	21.33980612885928
4	28.096782886948972	25.93704844571047	22.319371385841098	23.646797281499463
5	24.129221673318206	34.826258235476445	21.178660745738892	19.865859345466454
6	86.20827304944643	2.472827411639075	8.685202678030237	2.6336968608842577
7	88.02253827892685	2.2980692906751825	5.338492683613299	4.340899746784681
8	78.49288596480739	4.14553856313873	10.921462780659242	6.440112691394634
9	50.34367564156925	19.13665809125501	15.167073362539401	15.352592904636333
10	40.715422956042815	19.339377511573126	25.10014100221023	14.84505853017383
11	37.711330856673506	17.80178198096608	25.147969540579297	19.338917621781114
12	23.836271875807682	27.171484625424362	30.09997084298719	18.89227265578077
13	17.47535220659721	30.812156174894252	30.73047974783323	20.98201187067531
14	15.51953289913605	22.852291676822567	41.67024614221448	19.957929281826903
15	18.1527698702283	19.008440817242555	41.81704296382415	21.021746348704998
16	20.88819435310522	20.799619579164048	27.23035051880168	31.081835548929053
17	20.293924763869587	23.314204983917655	31.23874994596295	25.15312030624981
18	23.634748168948793	26.957819828056408	28.33252239433342	21.07490960866138
19	24.945250120261182	23.251016126495447	25.441379227881832	26.362354525361543
20	22.002691275062844	29.28826536008911	27.189604283229567	21.51943908161848
21	21.238262462783418	23.135491810742472	27.0248717597315	28.60137396674261
22	18.430083414810476	27.756648396778193	26.512462553473686	27.300805634937642
23	20.53113591858847	23.028613423079296	33.796105101373506	22.64414555695873
24	23.694993731702134	21.279560566105935	30.16481530366063	24.860630398531296
25	18.20179412205659	26.72713910838407	28.724164541209348	26.34690222835
26	18.92547669876391	27.02855087806758	28.27466825849853	25.771304164669978
27	22.40711835815665	24.83965942401563	31.497391964989507	21.25583025283821
28	20.417267206086734	26.03077398532216	27.79629089684948	25.755667911741632
29	23.09235414825191	24.643562416702462	27.527163390565086	24.73692004448054
30	29.52050970505428	23.0110456330245	22.995961247846566	24.47248341407465
31	30.733790954335703	23.157566520758966	23.163912999888705	22.944729525016623
32	27.043451307328713	25.52875828836378	22.34273378727522	25.085056617032294
33	25.291455155686492	22.342641809316817	26.43897216471045	25.926930870286245
34	23.274746439763174	23.664181115637447	27.866378101151838	25.194694343447537
35	30.733790954335703	22.647548741419605	25.236176402686862	21.38248390155783
36	24.70279622191338	27.390208210504436	25.455451855517342	22.451543712064844
37	23.99465792017601	25.216585097547224	28.581690683644574	22.207066298632196
38	20.70699777505319	25.771672076503588	28.299870219100693	25.221459929342533
39	25.539979599288827	23.61626059930998	28.21515851941241	22.628601281988782
40	19.630395771957208	20.64840781555108	31.699651495515614	28.021544916976094
41	21.895077063732447	24.214025350964892	25.41967242969895	28.47122515560371
42	22.646536983877184	22.098808241592984	27.47078090206463	27.783873872465204
43	24.53346480049521	25.71896870633921	26.48293762882663	23.26462886433895
44	21.648668113173358	26.44798600463385	26.18308948443595	25.72025639775684
45	24.83018569430022	24.257254991413856	26.277274913839648	24.635284400446277
46	24.856123478569593	22.302631397411922	28.003057347337286	24.838187776681195
47	22.507282354856482	22.168527534061738	30.06336361554317	25.26082649553861
48	22.665576421266408	21.025609422957885	27.45026981734097	28.85854433843474
49	22.16365270226643	22.783860075771443	30.300574770262056	24.751912451700075
50	21.4619528576172	23.86772833758118	28.504981066337265	26.165337738464356
51	21.2868268248197	20.363368122463132	29.44720327220785	28.90260178050932
52	22.10533867663953	19.064547371867803	32.72207848111279	26.108035470379875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	320.5
2	490.0
3	2306.5
4	4123.0
5	2730.5
6	1338.0
7	1417.0
8	1496.0
9	1571.0
10	1646.0
11	1591.0
12	1536.0
13	1444.5
14	1269.0
15	1185.0
16	1135.0
17	1085.0
18	1057.0
19	1029.0
20	1080.0
21	1131.0
22	1300.0
23	1469.0
24	1792.5
25	2116.0
26	2721.0
27	3326.0
28	4417.5
29	5509.0
30	5870.5
31	6232.0
32	7262.5
33	8293.0
34	10609.0
35	12925.0
36	14095.5
37	15266.0
38	17060.0
39	21692.5
40	24531.0
41	32688.5
42	40846.0
43	57001.5
44	73157.0
45	84742.0
46	96327.0
47	106739.5
48	117152.0
49	124126.5
50	131101.0
51	119951.0
52	108801.0
53	94429.0
54	80057.0
55	72518.0
56	64979.0
57	59358.0
58	53737.0
59	53618.5
60	53500.0
61	48463.0
62	43426.0
63	40692.0
64	31332.5
65	24707.0
66	20538.5
67	16370.0
68	13153.5
69	9937.0
70	8406.5
71	6876.0
72	6061.5
73	5247.0
74	4421.5
75	3596.0
76	2887.0
77	2178.0
78	1596.5
79	1015.0
80	919.5
81	824.0
82	510.5
83	197.0
84	155.0
85	113.0
86	59.5
87	6.0
88	11.5
89	9.5
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1087217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.44820803575731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.29975130951735	16.94254736841325
2	12.433085742843843	6.079333335349091
3	4.648235386821605	3.409230772085503
4	2.242190575179853	2.192701665512456
5	1.3397390793497919	1.6377109862788846
6	0.8618215929154778	1.264199615598321
7	0.6136627025375642	1.05020673907964
8	0.4975614850513527	0.9731589357692659
9	0.40301473562744156	0.8867689288285883
>10	6.742973517916212	43.050762752377594
>50	0.7752282436235366	11.991774144962896
>100	0.12914175922398927	5.36055226107578
>500	0.006419327212888231	1.08473959059759
>1k	0.006796934695999303	3.5164949570598134
>5k	3.7760748311107243E-4	0.5598179470113861
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	6064	0.5577543397500223	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	4356	0.40065598679932346	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	3947	0.36303700181288556	TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3854	0.354483051681495	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	3388	0.31162132306614043	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	3245	0.2984684750146475	Illumina Paired End PCR Primer 2 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2832	0.26048157819460144	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2340	0.21522842266079356	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2011	0.18496767434651962	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1689	0.15535077174105996	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	1361	0.12518200138518806	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1320	0.12141090509070407	TruSeq Adapter, Index 22 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1283	0.11800772062982826	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	1161	0.10678640970477835	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1133	0.104211026869521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3047229761859868	0.0
2	0.0	0.0	0.0	1.1972770845194658	0.0
3	0.0	0.0	0.0	1.9135094465962177	0.0
4	0.0	0.0	0.0	2.35362397755002	0.0
5	0.0	0.0	0.0	4.9162218765894945	0.0
6	0.0	0.0	0.0	5.408763843832464	0.0
7	0.0	0.0	0.0	7.623225170320184	0.0
8	0.0	0.0	0.0	9.973170029534122	0.0
9	0.0	0.0	0.0	12.099332515955876	0.0
10	9.197795840204854E-5	0.0	0.0	13.179797593304741	0.0
11	9.197795840204854E-5	0.0	0.0	14.309194944523494	0.0
12	9.197795840204854E-5	0.0	0.0	15.254176489146142	0.0
13	9.197795840204854E-5	0.0	0.0	15.645082812354847	0.0
14	9.197795840204854E-5	0.0	0.0	15.891859674747543	0.0
15	1.8395591680409707E-4	0.0	0.0	16.734101839835102	0.0
16	1.8395591680409707E-4	0.0	0.0	17.633094405256724	0.0
17	1.8395591680409707E-4	0.0	0.0	18.960336344998286	0.0
18	2.759338752061456E-4	0.0	0.0	19.598847332225304	0.0
19	2.759338752061456E-4	0.0	0.0	20.321794085265406	0.0
20	2.759338752061456E-4	0.0	0.0	21.204874463883474	0.0
21	2.759338752061456E-4	0.0	0.0	21.956978229737025	0.0
22	2.759338752061456E-4	0.0	0.0	22.771810963220773	0.0
23	2.759338752061456E-4	0.0	0.0	23.367276265915635	0.0
24	3.6791183360819415E-4	0.0	0.0	23.88731964272082	0.0
25	3.6791183360819415E-4	0.0	0.0	24.422355426745536	0.0
26	3.6791183360819415E-4	0.0	0.0	24.960610439314323	0.0
27	3.6791183360819415E-4	0.0	0.0	25.460970533021467	0.0
28	3.6791183360819415E-4	0.0	0.0	26.006491804304016	0.0
29	3.6791183360819415E-4	0.0	0.0	26.62265214763934	0.0
30	3.6791183360819415E-4	0.0	0.0	27.562666882508275	0.0
31	3.6791183360819415E-4	0.0	0.0	28.08684926744155	0.0
32	3.6791183360819415E-4	0.0	0.0	28.627127795095184	0.0
33	3.6791183360819415E-4	0.0	0.0	29.23225078342226	0.0
34	3.6791183360819415E-4	0.0	0.0	30.042392641027504	0.0
35	3.6791183360819415E-4	0.0	0.0	30.580739631554696	0.0
36	3.6791183360819415E-4	0.0	0.0	31.123041674293173	0.0
37	3.6791183360819415E-4	0.0	0.0	31.711424674191075	0.0
38	3.6791183360819415E-4	0.0	0.0	32.46536799921267	0.0
39	3.6791183360819415E-4	0.0	0.0	33.01401652108089	0.0
40	3.6791183360819415E-4	0.0	0.0	33.52348243266984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	20	6.3113024E-4	46.0	32
CGTATAC	25	3.4170334E-5	46.0	16
GCGAACC	20	6.3113024E-4	46.0	25
TAGCCGA	20	6.3113024E-4	46.0	34
ATCGTTA	25	3.4170334E-5	46.0	20
GTTCACG	20	6.3113024E-4	46.0	17
TTACGCT	25	3.4170334E-5	46.0	24
ACGTAGG	60	0.0	46.0	1
TAGGCGA	50	1.6370905E-11	46.0	19
AGTAGCG	30	1.86131E-6	46.0	32
CGACTAT	20	6.3113024E-4	46.0	40
CGTCAAA	20	6.3113024E-4	46.0	27
TAATCCG	20	6.3113024E-4	46.0	25
ATAGCGG	210	0.0	44.904762	1
CGTTTTT	1895	0.0	43.572563	1
TCACGAC	180	0.0	43.444443	24
CCCGCGA	80	0.0	43.125	28
AATACGG	65	0.0	42.46154	1
CGCATCG	60	1.8189894E-12	42.166668	20
CTAGCGG	350	0.0	42.057144	1
>>END_MODULE