FastQCFastQC Report
Thu 26 May 2016
SRR1545017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545017_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences632381
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA41670.6589382033932075No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC32490.5137725516737537No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT31960.5053915282084692TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG24240.3833132241481006No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT24100.3810993688931198Illumina Paired End PCR Primer 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT23260.3678162373632351TruSeq Adapter, Index 22 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21080.33334334839282015No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15450.24431474063895026No Hit
TGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTTG15440.24415660812073733TruSeq Adapter, Index 22 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.23672437976473043No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11950.18896835926443079No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT9140.14453312164660229No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC8920.1410542062459182No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT7420.11733432851398129TruSeq Adapter, Index 22 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC6960.1100602326761873TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC6790.10737197986656777No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA6530.10326053439303204No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6430.10167920921090293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGG453.092282E-1046.0000042
ACTTCGT206.30929E-446.029
TCGATAC206.30929E-446.024
TACGCGG501.6370905E-1146.01
TCGTCCA206.30929E-446.018
GTATCGG206.30929E-446.01
ACGTAGG253.415401E-546.01
TAGTCCG206.30929E-446.015
TAGTCAG850.046.033
CGCATCG700.045.99999620
TATCCGG351.0185977E-745.9999961
CATGCGG1900.043.5789451
ATAGCGG1750.043.371431
TAGTAGG850.043.2941171
CGTTTTT9700.043.1546361
CGGTCTA800.043.12530
ACGGGAT1550.043.0322574
AGACGGG2300.043.02
TTACGGG1500.042.933332
ATCGCCA750.042.9333323
Produced by FastQC (version 0.11.5)