##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545017_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 632381 Sequences flagged as poor quality 0 Sequence length 52 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.87121371451704 34.0 33.0 34.0 31.0 34.0 2 33.24385457501095 34.0 33.0 34.0 31.0 34.0 3 33.372054188851344 34.0 34.0 34.0 31.0 34.0 4 36.573598827289246 37.0 37.0 37.0 35.0 37.0 5 36.55784250317451 37.0 37.0 37.0 35.0 37.0 6 36.378026854064245 37.0 37.0 37.0 35.0 37.0 7 36.278857207917376 37.0 37.0 37.0 35.0 37.0 8 36.40809100842688 37.0 37.0 37.0 35.0 37.0 9 38.27638875930808 39.0 39.0 39.0 37.0 39.0 10 38.16037483732117 39.0 39.0 39.0 35.0 39.0 11 37.601347921585244 39.0 35.0 39.0 35.0 39.0 12 37.551974205423626 39.0 35.0 39.0 35.0 39.0 13 37.47781005438177 39.0 35.0 39.0 35.0 39.0 14 38.780151522578954 41.0 36.0 41.0 35.0 41.0 15 38.83709346106224 41.0 36.0 41.0 35.0 41.0 16 38.87182252471216 41.0 36.0 41.0 35.0 41.0 17 38.929972595634595 41.0 36.0 41.0 35.0 41.0 18 38.933971767020196 41.0 36.0 41.0 35.0 41.0 19 38.8948165741855 41.0 36.0 41.0 35.0 41.0 20 38.84095821980736 41.0 35.0 41.0 35.0 41.0 21 38.80273917148048 41.0 35.0 41.0 35.0 41.0 22 38.76484587614112 41.0 35.0 41.0 35.0 41.0 23 38.73574158616404 41.0 35.0 41.0 35.0 41.0 24 38.69316124298484 41.0 35.0 41.0 35.0 41.0 25 38.62772600694834 40.0 35.0 41.0 35.0 41.0 26 38.54476652524348 40.0 35.0 41.0 35.0 41.0 27 38.61102088772433 41.0 36.0 41.0 35.0 41.0 28 38.622700555519536 40.0 36.0 41.0 35.0 41.0 29 38.609744758302355 40.0 36.0 41.0 35.0 41.0 30 38.55393821129984 40.0 35.0 41.0 35.0 41.0 31 38.495161935605275 40.0 35.0 41.0 35.0 41.0 32 38.38749424792965 40.0 35.0 41.0 35.0 41.0 33 38.344953437880015 40.0 35.0 41.0 35.0 41.0 34 38.15248560598753 40.0 35.0 41.0 35.0 41.0 35 38.12363116538922 40.0 35.0 41.0 35.0 41.0 36 38.03588659368324 40.0 35.0 41.0 35.0 41.0 37 37.993546611931734 40.0 35.0 41.0 35.0 41.0 38 37.881912960699324 40.0 35.0 41.0 35.0 41.0 39 37.880739617414186 40.0 35.0 41.0 35.0 41.0 40 37.844421321956226 40.0 35.0 41.0 35.0 41.0 41 37.81522215246821 40.0 35.0 41.0 35.0 41.0 42 37.773367637547615 40.0 35.0 41.0 35.0 41.0 43 37.724411391234085 40.0 35.0 41.0 35.0 41.0 44 37.562799957620484 39.0 35.0 41.0 35.0 41.0 45 37.548451012917845 39.0 35.0 41.0 35.0 41.0 46 37.46210907664841 39.0 35.0 41.0 35.0 41.0 47 37.371685740083905 39.0 35.0 41.0 35.0 41.0 48 37.29980502260504 39.0 35.0 41.0 35.0 41.0 49 37.233844786608074 39.0 35.0 41.0 34.0 41.0 50 37.14115857370794 38.0 35.0 41.0 34.0 41.0 51 37.02859194061808 38.0 35.0 41.0 34.0 41.0 52 36.52788746024944 37.0 35.0 40.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 2.0 14 4.0 15 11.0 16 18.0 17 16.0 18 52.0 19 96.0 20 143.0 21 223.0 22 332.0 23 645.0 24 984.0 25 1646.0 26 2488.0 27 2890.0 28 2976.0 29 3008.0 30 3500.0 31 4259.0 32 5749.0 33 8730.0 34 34245.0 35 106786.0 36 23614.0 37 38779.0 38 74871.0 39 315016.0 40 1296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.93618720360036 20.75267916018982 22.7608040089756 22.550329627234213 2 28.127031014530797 24.44428279787027 25.76294986724775 21.66573632035118 3 29.37706857100387 23.95280693126454 25.328876104753306 21.341248392978287 4 27.888883442102152 25.250284243201488 23.086240731457778 23.774591583238585 5 23.91501325941165 34.06348388076176 21.410984833510177 20.610518026316413 6 84.91652975026132 2.4916940894808666 9.296927010773569 3.294849149484251 7 86.8084271981606 2.1963025454591456 6.071498036784787 4.923772219595465 8 76.28834515901015 4.192567455378956 11.924773198435753 7.594314187175137 9 49.93524473379181 18.501662763429007 15.671406952454294 15.891685550324883 10 39.48331780999113 18.927988032531022 26.56515613214186 15.023538025335991 11 36.53224875510175 17.567257713308905 25.304049299393878 20.596444232195466 12 23.18649674800476 26.16128568062608 30.821767257397042 19.830450313972115 13 17.29700923968304 29.79169203375813 31.01547959220786 21.89581913435097 14 15.247453671125477 22.618801007620405 40.863972826508075 21.269772494746046 15 17.742152278452387 18.80243081306997 41.63360379265031 21.821813115827325 16 20.42091713697913 21.503176091628305 27.56265605702891 30.51325071436365 17 19.647332857881562 23.71798014171836 31.450185884775163 25.18450111562491 18 22.970329595607712 27.15166964219355 28.18807016656098 21.689930595637758 19 24.39968942773423 23.15660970206252 25.852294740038044 26.5914061301652 20 21.39912489464421 29.623280901861378 27.30063047435011 21.676963729144298 21 20.827317708786317 22.9469259829122 26.89075098714224 29.335005321159237 22 18.25750615530827 27.788943690591587 26.69514106211287 27.258409091987268 23 20.16885390294775 22.91276935897821 33.93444774590002 22.98392899217402 24 23.69346960139536 21.724087219571743 29.48965892397147 25.092784255061428 25 18.8248856306562 26.472806741505515 28.41815298056077 26.284154647277514 26 19.136564824053853 26.485931740517188 27.907543079251273 26.469960356177687 27 23.31569101538471 24.351300877161076 31.035246156984474 21.29776195046973 28 21.452573685800175 25.436880614692726 27.34364251930403 25.766903180203073 29 23.656940989688177 24.082317463680912 27.161790123359175 25.098951423271732 30 29.700449570749278 23.0571443481066 22.428251323173846 24.814154757970275 31 30.811014246158564 22.691383833480135 22.59571365996132 23.901888260399982 32 26.447031141036813 25.645141141179128 22.4301489133924 25.477678804391658 33 25.598650180824535 22.233906458290175 26.00188810226746 26.16555525861783 34 24.326632204319864 23.116918439991082 27.79985483434828 24.756594521340773 35 30.542663362751256 22.29004350225576 25.825728476978277 21.341564658014708 36 25.336782730663955 27.428085284029724 24.8573249354424 22.377807049863925 37 24.751534280757962 24.721963499852144 28.989485768864025 21.53701645052587 38 20.738763498587087 25.44462910808516 28.13003553237684 25.686571860950913 39 25.876805280361047 23.194877771470047 28.387949669582103 22.5403672785868 40 19.706632552211403 20.286504496498157 32.09758041433882 27.90928253695162 41 21.94199382966914 24.04310059916411 25.798529683845654 28.216375887321092 42 22.352189581913436 21.73831914621091 27.51584883163789 28.39364244023777 43 24.89638366744099 25.707761618391444 26.34804018463553 23.047814529532037 44 22.030389907350155 25.427392663599953 26.812949788181488 25.729267640868404 45 24.43890629225103 23.58420003131024 26.450351923919285 25.526541752519442 46 24.983514684976306 21.323379418420224 28.10536685953563 25.58773903706784 47 22.29273175506538 21.88727997836747 30.411255240116326 25.408733026450825 48 22.37891397749142 20.42629364259837 27.236744936992096 29.95804744291811 49 22.00777695724571 21.864825160781237 30.518469087464677 25.608928794508373 50 21.545081208954727 22.99088682297539 28.8814812589246 26.58255070914528 51 21.590939639236474 19.643537677444453 29.594026385991988 29.17149629732709 52 21.868462208700134 19.08912506858998 32.98059239603973 26.061820326670155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 188.0 2 315.0 3 1458.5 4 2602.0 5 1735.0 6 868.0 7 905.0 8 942.0 9 972.0 10 1002.0 11 960.5 12 919.0 13 863.0 14 763.0 15 719.0 16 702.0 17 685.0 18 628.5 19 572.0 20 591.0 21 610.0 22 724.5 23 839.0 24 1026.0 25 1213.0 26 1499.0 27 1785.0 28 2278.0 29 2771.0 30 2996.5 31 3222.0 32 3792.0 33 4362.0 34 5441.0 35 6520.0 36 7270.0 37 8020.0 38 9236.5 39 11870.5 40 13288.0 41 17427.5 42 21567.0 43 30410.0 44 39253.0 45 46724.0 46 54195.0 47 62097.5 48 70000.0 49 74954.0 50 79908.0 51 73093.0 52 66278.0 53 57017.0 54 47756.0 55 42674.0 56 37592.0 57 34224.0 58 30856.0 59 30462.0 60 30068.0 61 27707.0 62 25346.0 63 25170.0 64 19807.0 65 14620.0 66 12159.0 67 9698.0 68 7751.0 69 5804.0 70 4953.0 71 4102.0 72 3580.5 73 3059.0 74 2611.0 75 2163.0 76 1732.0 77 1301.0 78 945.0 79 589.0 80 518.5 81 448.0 82 283.5 83 119.0 84 97.5 85 76.0 86 39.0 87 2.0 88 6.5 89 5.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 632381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.474735188712735 #Duplication Level Percentage of deduplicated Percentage of total 1 70.94734804383603 20.911542959335037 2 11.05240121940016 6.5153319828245095 3 3.885411243582277 3.4356440251150393 4 1.9416280597804938 2.2891589158801664 5 1.2010118383736894 1.769975294728678 6 0.8709768125223156 1.5403086542762612 7 0.7314781391696411 1.5092087113850274 8 0.637190672311705 1.502482106488427 9 0.5376357836091862 1.4262005114871146 >10 7.899353851557319 45.678763609342106 >50 0.21265557862220996 4.1793850968201856 >100 0.07106464905855239 3.9976933990500654 >500 0.0059220540882127 1.172347828054693 >1k 0.0059220540882127 4.071956905212707 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA 4167 0.6589382033932075 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC 3249 0.5137725516737537 No Hit CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT 3196 0.5053915282084692 TruSeq Adapter, Index 22 (95% over 23bp) GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG 2424 0.3833132241481006 No Hit CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT 2410 0.3810993688931198 Illumina Paired End PCR Primer 2 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT 2326 0.3678162373632351 TruSeq Adapter, Index 22 (95% over 23bp) GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 2108 0.33334334839282015 No Hit GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 1545 0.24431474063895026 No Hit TGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTTG 1544 0.24415660812073733 TruSeq Adapter, Index 22 (96% over 25bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1497 0.23672437976473043 No Hit GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 1195 0.18896835926443079 No Hit GGGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT 914 0.14453312164660229 No Hit ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 892 0.1410542062459182 No Hit TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT 742 0.11733432851398129 TruSeq Adapter, Index 22 (95% over 23bp) GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 696 0.1100602326761873 TruSeq Adapter, Index 22 (95% over 22bp) CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 679 0.10737197986656777 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA 653 0.10326053439303204 No Hit AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 643 0.10167920921090293 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3886897297673396 0.0 2 0.0 0.0 0.0 1.488659526456361 0.0 3 0.0 0.0 0.0 2.304781452953204 0.0 4 0.0 0.0 0.0 2.804954608060647 0.0 5 0.0 0.0 0.0 5.686129089899918 0.0 6 0.0 0.0 0.0 6.246550734446481 0.0 7 0.0 0.0 0.0 8.702348742293017 0.0 8 0.0 0.0 0.0 11.271211500661785 0.0 9 0.0 0.0 0.0 13.708982401432047 0.0 10 0.0 0.0 0.0 14.939727790683149 0.0 11 0.0 0.0 0.0 16.17221263763459 0.0 12 0.0 0.0 0.0 17.225533341450802 0.0 13 0.0 0.0 0.0 17.643161322051107 0.0 14 0.0 0.0 0.0 17.917363108632298 0.0 15 0.0 0.0 0.0 18.81429075193594 0.0 16 0.0 0.0 0.0 19.769727426978356 0.0 17 0.0 0.0 0.0 21.053605342348995 0.0 18 0.0 0.0 0.0 21.70321372716764 0.0 19 0.0 0.0 0.0 22.442799514849433 0.0 20 1.5813251821291278E-4 0.0 0.0 23.328657881878172 0.0 21 1.5813251821291278E-4 0.0 0.0 24.115999690060264 0.0 22 1.5813251821291278E-4 0.0 0.0 24.990630648295884 0.0 23 1.5813251821291278E-4 0.0 0.0 25.602603493779856 0.0 24 1.5813251821291278E-4 0.0 0.0 26.121436286036424 0.0 25 1.5813251821291278E-4 0.0 0.0 26.667626003943823 0.0 26 1.5813251821291278E-4 0.0 0.0 27.204485903276662 0.0 27 1.5813251821291278E-4 0.0 0.0 27.70655664860266 0.0 28 1.5813251821291278E-4 0.0 0.0 28.25638341442896 0.0 29 1.5813251821291278E-4 0.0 0.0 28.846533972399552 0.0 30 1.5813251821291278E-4 0.0 0.0 29.755637819605585 0.0 31 1.5813251821291278E-4 0.0 0.0 30.279056454890327 0.0 32 1.5813251821291278E-4 0.0 0.0 30.822716052506323 0.0 33 1.5813251821291278E-4 0.0 0.0 31.44259552390094 0.0 34 1.5813251821291278E-4 0.0 0.0 32.22155630861775 0.0 35 1.5813251821291278E-4 0.0 0.0 32.76157885831484 0.0 36 1.5813251821291278E-4 0.0 0.0 33.30523845593084 0.0 37 1.5813251821291278E-4 0.0 0.0 33.865185702922766 0.0 38 1.5813251821291278E-4 0.0 0.0 34.638928114538544 0.0 39 1.5813251821291278E-4 0.0 0.0 35.18195518208169 0.0 40 1.5813251821291278E-4 0.0 0.0 35.68481658999875 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGGG 45 3.092282E-10 46.000004 2 ACTTCGT 20 6.30929E-4 46.0 29 TCGATAC 20 6.30929E-4 46.0 24 TACGCGG 50 1.6370905E-11 46.0 1 TCGTCCA 20 6.30929E-4 46.0 18 GTATCGG 20 6.30929E-4 46.0 1 ACGTAGG 25 3.415401E-5 46.0 1 TAGTCCG 20 6.30929E-4 46.0 15 TAGTCAG 85 0.0 46.0 33 CGCATCG 70 0.0 45.999996 20 TATCCGG 35 1.0185977E-7 45.999996 1 CATGCGG 190 0.0 43.578945 1 ATAGCGG 175 0.0 43.37143 1 TAGTAGG 85 0.0 43.294117 1 CGTTTTT 970 0.0 43.154636 1 CGGTCTA 80 0.0 43.125 30 ACGGGAT 155 0.0 43.032257 4 AGACGGG 230 0.0 43.0 2 TTACGGG 150 0.0 42.93333 2 ATCGCCA 75 0.0 42.93333 23 >>END_MODULE