FastQCFastQC Report
Thu 26 May 2016
SRR1545016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545016_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences450052
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT20650.4588358678552701No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT15600.3466266120359425No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT15200.33773875018886707Illumina Single End Adapter 2 (95% over 21bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG9420.20930914649862684No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.205087412121266No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC6390.1419835930070303No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT5800.128873996782594No Hit
GCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC4950.10998729035755868No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC4800.10665434216490538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG351.0177064E-746.0000041
AATGCGG351.0177064E-746.0000041
CGGGATA351.0177064E-746.0000045
CTCGAGG351.0177064E-746.0000041
CCCTGCG206.307343E-446.043
TCACGAC206.307343E-446.024
CCTTCGG253.4138204E-546.011
CGGGTTC253.4138204E-546.05
AATCATA253.4138204E-546.043
GTCGAGG206.307343E-446.01
ATAGGCC206.307343E-446.014
CGCGGGT253.4138204E-546.03
CATGCGG650.046.01
CTATAGG600.046.01
GAACTGC253.4138204E-546.023
CGGTTAG206.307343E-446.024
CGTTGAT206.307343E-446.024
TCTTAGG600.046.01
ATTCGAA253.4138204E-546.041
CACGAGG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)