FastQCFastQC Report
Thu 26 May 2016
SRR1545015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545015_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences266822
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT16040.6011498302238947No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT11330.42462765439131706Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT10610.39764337273538164No Hit
TGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTTG7440.27883757711133267Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.23648724617910066No Hit
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGG5730.21474990817848602No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4330.16228047162527828No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC4070.15253614769396825No Hit
GCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC3770.14129269700399516No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT3570.13379706321067977No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC3310.12405273927936977No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT3060.11468319703772552No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC2980.11168494352039937No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT2760.10343974634775244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAGG351.0156327E-746.0000041
AGGGTCC351.0156327E-746.0000045
GTTACGG351.0156327E-746.0000041
ATTGGGC351.0156327E-746.0000043
GTCCCCC700.046.0000049
CACGTGA750.046.00000442
AACACGT750.046.00000440
ATTTAGG253.4100558E-546.01
GTACCGG206.302702E-446.01
ACACGAC800.046.025
CATATGG206.302702E-446.01
ATAGGTT206.302702E-446.011
GTCGAGG206.302702E-446.01
GGGCTCG206.302702E-446.06
GCGCGAC253.4100558E-546.08
CTTCACG206.302702E-446.046
ATAATGG206.302702E-446.01
GACAATA405.5842975E-946.020
TATGGGT301.8559949E-646.03
GCTATCA206.302702E-446.08
Produced by FastQC (version 0.11.5)