FastQCFastQC Report
Thu 26 May 2016
SRR1545014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545014_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences293706
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT15230.5185457566409948TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTT12670.4313837647170981TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT12490.4252551871599491TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.232204994109756No Hit
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT5670.19305019305019305TruSeq Adapter, Index 13 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC4850.16513111751206988TruSeq Adapter, Index 16 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT4280.14572395524776477No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC4130.14061680728347395TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC4100.13959537769061578TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC3880.13210489400965592TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT3720.1266572695144124TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG3340.11371916133820896No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA3300.11235725521439807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGG750.046.0000041
CAGGTAG253.4109027E-546.04
CGGGTGC206.303744E-446.05
ATCTCGG206.303744E-446.01
GTACCGA206.303744E-446.08
GGTACCG206.303744E-446.07
AGTCCAT600.046.038
ACTGCGG650.046.01
CATGCGG453.092282E-1046.01
TAGCGGC206.303744E-446.02
ACCGAGG301.8566388E-646.01
TACGGGA453.092282E-1046.03
GCGCATT253.4109027E-546.010
AACGGGA301.8566388E-646.03
TCGGATC206.303744E-446.021
TACGCGG405.5861165E-946.01
CACTAGG301.8566388E-646.01
GCTTAGG600.046.01
GTCACGG405.5861165E-946.01
GAAACGT206.303744E-446.08
Produced by FastQC (version 0.11.5)