FastQCFastQC Report
Thu 26 May 2016
SRR1545013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545013_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences173857
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT12020.6913727948831512TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTT10150.5838131337823613TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT8700.5004112575277383TruSeq Adapter, Index 13 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTTG5880.33820898784633346TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.24617933128950803No Hit
ACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC3360.1932622787693334TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT3200.18405931311365087TruSeq Adapter, Index 13 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC2860.16450301109532547TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA2540.1460970797839604No Hit
AGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC2480.14264596766307944TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC2450.14092041160263896TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT2370.13631892877479768TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG1920.11043558786819052No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT1840.10583410504034925No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA1770.10180780756598813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.01288606E-746.0000045
ACTGCGG351.01288606E-746.0000041
CGTAGGG351.01288606E-746.0000042
GTCACGG351.01288606E-746.0000041
CGGGCAA700.046.0000045
GCAGGGT351.01288606E-746.0000043
AAGATTG351.01288606E-746.00000413
CTAGCGG351.01288606E-746.0000041
TCAAAGG351.01288606E-746.0000041
CGTGGGA206.29661E-446.03
AACCGGG206.29661E-446.02
AATCCGG206.29661E-446.01
TCGTTGA253.4051205E-546.023
ACAAATG206.29661E-446.026
ACACGAC253.4051205E-546.025
ATGATCG253.4051205E-546.026
TTAATCG253.4051205E-546.019
AAGAGTG253.4051205E-546.035
GTCGAAT253.4051205E-546.029
ATAGGGT405.5642886E-946.03
Produced by FastQC (version 0.11.5)