##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545013_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	173857
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86134006683654	34.0	33.0	34.0	31.0	34.0
2	33.215171088883395	34.0	33.0	34.0	31.0	34.0
3	33.33727143572016	34.0	34.0	34.0	31.0	34.0
4	36.589122094594984	37.0	37.0	37.0	35.0	37.0
5	36.58437106357524	37.0	37.0	37.0	35.0	37.0
6	36.38171600798357	37.0	37.0	37.0	35.0	37.0
7	36.19567805725395	37.0	37.0	37.0	35.0	37.0
8	36.34432896000736	37.0	37.0	37.0	35.0	37.0
9	38.21934118269613	39.0	39.0	39.0	37.0	39.0
10	38.11444462978195	39.0	39.0	39.0	35.0	39.0
11	37.51323213905681	39.0	35.0	39.0	35.0	39.0
12	37.47275059387888	39.0	35.0	39.0	35.0	39.0
13	37.463927250556495	39.0	35.0	39.0	35.0	39.0
14	38.71497840178998	41.0	35.0	41.0	35.0	41.0
15	38.760032670528076	41.0	35.0	41.0	35.0	41.0
16	38.79945012280207	41.0	35.0	41.0	35.0	41.0
17	38.892687668601205	41.0	36.0	41.0	35.0	41.0
18	38.8809021207084	41.0	36.0	41.0	35.0	41.0
19	38.82562680823896	41.0	36.0	41.0	35.0	41.0
20	38.75957252224529	41.0	35.0	41.0	35.0	41.0
21	38.725947186480845	41.0	35.0	41.0	35.0	41.0
22	38.69303508055471	41.0	35.0	41.0	35.0	41.0
23	38.66911312170347	41.0	35.0	41.0	35.0	41.0
24	38.64178606555963	41.0	35.0	41.0	35.0	41.0
25	38.60534232156312	41.0	35.0	41.0	35.0	41.0
26	38.55077448707846	40.0	35.0	41.0	35.0	41.0
27	38.631495999585866	41.0	36.0	41.0	35.0	41.0
28	38.6514836906193	41.0	36.0	41.0	35.0	41.0
29	38.648728552776134	41.0	36.0	41.0	35.0	41.0
30	38.59389613302887	41.0	35.0	41.0	35.0	41.0
31	38.53558384189305	41.0	35.0	41.0	35.0	41.0
32	38.47214664925772	41.0	35.0	41.0	35.0	41.0
33	38.380531126155404	41.0	35.0	41.0	35.0	41.0
34	38.29221716698206	41.0	35.0	41.0	35.0	41.0
35	38.23313412747258	40.0	35.0	41.0	35.0	41.0
36	38.18815463283043	40.0	35.0	41.0	35.0	41.0
37	38.15192370741472	40.0	35.0	41.0	35.0	41.0
38	38.126057622068714	40.0	35.0	41.0	35.0	41.0
39	38.08204443882041	40.0	35.0	41.0	35.0	41.0
40	38.04114300833444	40.0	35.0	41.0	35.0	41.0
41	38.004020545620826	40.0	35.0	41.0	35.0	41.0
42	37.92366715173965	40.0	35.0	41.0	35.0	41.0
43	37.842399213146436	40.0	35.0	41.0	35.0	41.0
44	37.6573505812248	40.0	35.0	41.0	35.0	41.0
45	37.70098989399334	40.0	35.0	41.0	35.0	41.0
46	37.68254945156077	40.0	35.0	41.0	35.0	41.0
47	37.623719493606814	40.0	35.0	41.0	35.0	41.0
48	37.59529958529136	40.0	35.0	41.0	35.0	41.0
49	37.558407196719145	39.0	35.0	41.0	35.0	41.0
50	37.47980236631255	39.0	35.0	41.0	35.0	41.0
51	37.412114553914996	39.0	35.0	41.0	35.0	41.0
52	36.99194740505128	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	2.0
16	1.0
17	4.0
18	3.0
19	25.0
20	38.0
21	67.0
22	88.0
23	138.0
24	311.0
25	517.0
26	751.0
27	828.0
28	772.0
29	814.0
30	898.0
31	1192.0
32	1567.0
33	2386.0
34	7565.0
35	32874.0
36	5616.0
37	9121.0
38	16169.0
39	90667.0
40	1441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.862967841386887	21.972655688295553	24.09451445728386	22.0698620130337
2	26.83239673984942	24.074958155265534	26.42516550958546	22.667479595299586
3	25.855156824286624	23.822451785087743	27.286217983745264	23.036173406880366
4	25.97824648993138	26.67307039693541	23.74307620630748	23.605606906825724
5	23.000511914964598	34.5266512133535	21.615465583784374	20.857371287897525
6	83.9747608666893	2.4002484800727033	9.65851245563883	3.966478197599176
7	84.04838459193475	2.1759261922154414	6.947088699333361	6.828600516516447
8	71.92232696986603	4.622764685920037	14.64536947031181	8.809538873902115
9	46.77349775965305	18.006177490696377	17.378650270049523	17.841674479601053
10	38.61104240841611	18.774049937592387	29.207912249722472	13.406995404269026
11	36.52024364851573	16.624007086283555	26.361895120702645	20.493854144498062
12	20.027378822825657	29.612267553219024	31.776690038364862	18.583663585590458
13	14.320389745595518	32.70388882817488	31.682359640394115	21.293361785835486
14	12.820881529072745	22.173970562013608	44.46988041896501	20.535267489948637
15	14.667801699097534	17.701904438705373	45.42008662291423	22.21020723928286
16	14.641343172837448	22.658851814997384	30.20815957942447	32.491645432740704
17	17.82902040182449	22.979230056885832	35.14037398551683	24.051375555772847
18	21.874874178203925	28.215142329615716	29.102078144682125	20.80790534749823
19	23.167315667473844	23.101744537177105	27.079151256492406	26.651788538856646
20	18.013079714938137	32.20520312670758	27.501912491300324	22.279804667053956
21	18.3852246386398	24.779560213278728	26.261237683843618	30.57397746423785
22	16.323760331766913	30.68671379351996	23.865015501245278	29.12451037346785
23	16.46180481660215	22.611686616012012	37.179406063604	23.747102503781843
24	19.085225213825154	22.61456254277941	33.56264056092076	24.737571682474677
25	14.636741690009606	29.2332204052756	30.840288282899163	25.289749621815634
26	17.351616558435957	27.597393259978027	27.02220790649787	28.028782275088147
27	20.51628637328379	26.06279873689296	33.64201613970102	19.778898750122227
28	17.25613578975825	24.223355976463417	28.898462529550144	29.62204570422819
29	18.845372921423927	29.8854805961221	25.07865659708841	26.190489885365558
30	27.42253691252006	24.811770593073618	21.05638542020166	26.709307074204663
31	25.18506588748224	26.29172250757807	20.73025532477841	27.79295628016128
32	23.51702836238978	26.802487101468447	22.114726470605152	27.56575806553662
33	17.834197070005807	28.235273816987522	25.383504834432895	28.54702427857377
34	18.84134662394957	21.751209327205693	26.600021857043433	32.80742219180131
35	22.892952253863807	24.179066704245443	26.245707679299656	26.682273362591097
36	18.824091063345165	26.906595650448356	26.186463587891197	28.082849698315282
37	17.416612503379213	27.570934733717937	28.774797678551913	26.23765508435093
38	16.35424515550136	24.911277659225686	28.961157733079485	29.77331945219347
39	22.90560633164037	21.408974041885	31.483920693443462	24.201498933031168
40	15.851533156559702	19.483253478433422	34.18671666944673	30.478496695560143
41	20.304042977849612	23.98868035224351	25.64866528238725	30.05861138751963
42	20.828612020223517	19.21234117694427	30.32147109406006	29.63757570877215
43	22.53806289076655	24.682929073894062	27.520318422611687	25.258689612727704
44	16.150054355015904	27.96378633014489	27.53067175897433	28.355487555864876
45	20.592786025296654	26.432067733827225	26.552281472704582	26.422864768171543
46	22.458112126632805	20.25975370563164	29.072743691654633	28.209390476080916
47	19.653508343063553	18.367393892681918	33.99978142956568	27.979316334688853
48	19.346359364305147	17.096234261490764	29.696819800180606	33.86058657402348
49	19.649482045589192	20.48292562278194	32.30701093427357	27.5605813973553
50	19.235923776436955	22.007166809504362	30.300189235981296	28.45672017807739
51	17.8025618755644	18.189086433103068	32.76485847564378	31.243493215688755
52	19.271585268352727	17.558683285688815	37.7534410463772	25.416290399581264
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	51.0
2	79.0
3	434.0
4	789.0
5	524.5
6	260.0
7	270.5
8	281.0
9	312.5
10	344.0
11	336.0
12	328.0
13	314.0
14	285.5
15	271.0
16	238.5
17	206.0
18	197.5
19	189.0
20	203.0
21	217.0
22	225.5
23	234.0
24	291.0
25	348.0
26	442.5
27	537.0
28	738.5
29	940.0
30	989.0
31	1038.0
32	1200.0
33	1362.0
34	1649.0
35	1936.0
36	2193.5
37	2451.0
38	2747.0
39	3675.0
40	4307.0
41	5708.5
42	7110.0
43	9816.0
44	12522.0
45	15394.5
46	18267.0
47	21258.5
48	24250.0
49	24602.5
50	24955.0
51	22016.5
52	19078.0
53	16015.0
54	12952.0
55	10927.5
56	8903.0
57	7660.0
58	6417.0
59	5848.5
60	5280.0
61	4902.0
62	4524.0
63	4006.0
64	2891.5
65	2295.0
66	1859.0
67	1423.0
68	1200.5
69	978.0
70	841.5
71	705.0
72	593.5
73	482.0
74	416.5
75	351.0
76	287.0
77	223.0
78	157.0
79	91.0
80	62.5
81	34.0
82	32.5
83	31.0
84	21.5
85	12.0
86	6.5
87	1.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	173857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.98072553880488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75911676145869	30.84258902431308
2	9.840080294412846	8.458675808279217
3	4.066912010705922	5.243964867678609
4	2.9066577450652393	4.997210351035621
5	2.3860823017731683	5.1277774262756175
6	1.9551689528270324	5.042074808607073
7	1.6232853797256606	4.88389883640003
8	1.2566075610572098	4.320792375342954
9	0.936768149882904	3.6236677269250013
>10	3.1609233857477417	20.509384149042027
>50	0.0588825694212111	1.622022696814048
>100	0.04416192706590833	3.2141357552471286
>500	0.0026764804282368685	0.8386202453740718
>1k	0.0026764804282368685	1.2751859286655125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1202	0.6913727948831512	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTT	1015	0.5838131337823613	TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	870	0.5004112575277383	TruSeq Adapter, Index 13 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCTTG	588	0.33820898784633346	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	428	0.24617933128950803	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	336	0.1932622787693334	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	320	0.18405931311365087	TruSeq Adapter, Index 13 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	286	0.16450301109532547	TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	254	0.1460970797839604	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	248	0.14264596766307944	TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	245	0.14092041160263896	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	237	0.13631892877479768	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	192	0.11043558786819052	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	184	0.10583410504034925	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	177	0.10180780756598813	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.597042396912405	0.0
2	0.0	0.0	0.0	2.13451284676487	0.0
3	0.0	0.0	0.0	3.310766894631795	0.0
4	0.0	0.0	0.0	4.083816009709129	0.0
5	0.0	0.0	0.0	7.99507641337421	0.0
6	0.0	0.0	0.0	8.731313665828813	0.0
7	0.0	0.0	0.0	12.3728121387117	0.0
8	0.0	0.0	0.0	16.178813622689912	0.0
9	0.0	0.0	0.0	19.9710106581846	0.0
10	0.0	0.0	0.0	21.63559707115618	0.0
11	0.0	0.0	0.0	23.251292729081946	0.0
12	0.0	0.0	0.0	24.579395710267633	0.0
13	0.0	0.0	0.0	25.082107709209293	0.0
14	0.0	0.0	0.0	25.45942930109228	0.0
15	0.0	0.0	0.0	26.731164117636908	0.0
16	0.0	0.0	0.0	28.07594747407352	0.0
17	0.0	0.0	0.0	29.95162691177232	0.0
18	0.0	0.0	0.0	30.67866119857124	0.0
19	0.0	0.0	0.0	31.723197800491207	0.0
20	0.0	0.0	0.0	32.86148961502844	0.0
21	0.0	0.0	0.0	33.81399656039159	0.0
22	0.0	0.0	0.0	34.9499876335149	0.0
23	0.0	0.0	0.0	35.66839414001162	0.0
24	5.75185353480159E-4	0.0	0.0	36.35401508135997	0.0
25	0.001150370706960318	0.0	0.0	36.99017008230902	0.0
26	0.001150370706960318	0.0	0.0	37.61942285901632	0.0
27	0.001150370706960318	0.0	0.0	38.21589007057524	0.0
28	0.001150370706960318	0.0	0.0	38.767492824562716	0.0
29	0.001150370706960318	0.0	0.0	39.508906745198644	0.0
30	0.001150370706960318	0.0	0.0	40.251471036541524	0.0
31	0.001150370706960318	0.0	0.0	40.80192341982204	0.0
32	0.001150370706960318	0.0	0.0	41.42657471370149	0.0
33	0.001150370706960318	0.0	0.0	41.95517005354976	0.0
34	0.001150370706960318	0.0	0.0	42.69945990095308	0.0
35	0.001150370706960318	0.0	0.0	43.25796487918232	0.0
36	0.001150370706960318	0.0	0.0	43.768729473072696	0.0
37	0.001150370706960318	0.0	0.0	44.30077592504185	0.0
38	0.001150370706960318	0.0	0.0	44.83914941589927	0.0
39	0.001150370706960318	0.0	0.0	45.424112920388595	0.0
40	0.001150370706960318	0.0	0.0	46.006200498110516	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.01288606E-7	46.000004	5
ACTGCGG	35	1.01288606E-7	46.000004	1
CGTAGGG	35	1.01288606E-7	46.000004	2
GTCACGG	35	1.01288606E-7	46.000004	1
CGGGCAA	70	0.0	46.000004	5
GCAGGGT	35	1.01288606E-7	46.000004	3
AAGATTG	35	1.01288606E-7	46.000004	13
CTAGCGG	35	1.01288606E-7	46.000004	1
TCAAAGG	35	1.01288606E-7	46.000004	1
CGTGGGA	20	6.29661E-4	46.0	3
AACCGGG	20	6.29661E-4	46.0	2
AATCCGG	20	6.29661E-4	46.0	1
TCGTTGA	25	3.4051205E-5	46.0	23
ACAAATG	20	6.29661E-4	46.0	26
ACACGAC	25	3.4051205E-5	46.0	25
ATGATCG	25	3.4051205E-5	46.0	26
TTAATCG	25	3.4051205E-5	46.0	19
AAGAGTG	25	3.4051205E-5	46.0	35
GTCGAAT	25	3.4051205E-5	46.0	29
ATAGGGT	40	5.5642886E-9	46.0	3
>>END_MODULE