FastQCFastQC Report
Thu 26 May 2016
SRR1545012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545012_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences742293
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT31840.4289411324099783No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT26610.3584837793162538Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT23140.3117367400743372No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.19412819466167672No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC9560.12879011387686534No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT9190.1238055592602921No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC8040.1083130246412131No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT7680.1034631877169797No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC7580.10211601079358151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGA351.0189069E-746.00000411
CACAACG351.0189069E-746.00000411
ATGGGCG850.046.04
ACCGGTT206.310002E-446.09
CAACGAC301.860506E-646.011
ATAGCGG1150.046.01
ATTGTCG206.310002E-446.035
TACGGGA1200.046.03
TAGCACG301.860506E-646.01
AATGCGG453.092282E-1046.01
TGCGATA253.4159784E-546.024
TGTTCGG206.310002E-446.01
GTCCGCG206.310002E-446.046
AACGACG253.4159784E-546.012
GCGATAA253.4159784E-546.08
CGTACAT206.310002E-446.034
ACCGTGT206.310002E-446.026
CGGTCTA206.310002E-446.030
CCGGTAT206.310002E-446.042
ATAACGG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)