FastQCFastQC Report
Thu 26 May 2016
SRR1545011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545011_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences491194
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT28550.581236741491142No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT22120.4503312336877079Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT19200.39088425347215155No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTTG13600.2768763462094407Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA10690.21763295154256768No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.20216044984262835No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT7100.1445457395652227No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC7100.1445457395652227No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC6750.13742024536130326No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC6010.12235491475873075No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4960.10097843214697248No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT4940.10057126104960565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG351.0179792E-746.0000041
GTGCACG206.3079066E-446.020
GGTACAC206.3079066E-446.07
AGATCGT206.3079066E-446.010
GTACAGG1200.046.01
AAATACG206.3079066E-446.027
CACGGGT253.4142773E-546.03
TACGGGA650.046.03
ACGGGTA453.092282E-1046.04
ACGGGCC206.3079066E-446.04
AATGCGG650.046.01
ACCTAGG453.092282E-1046.01
CTAAACG206.3079066E-446.045
GGTATGT206.3079066E-446.07
ACGCTAA206.3079066E-446.034
ATGCGCT206.3079066E-446.02
TTCGGAC253.4142773E-546.013
GGCATAG405.6006684E-946.07
ATAACGG206.3079066E-446.01
ACGTAGG551.8189894E-1246.01
Produced by FastQC (version 0.11.5)