##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545011_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	491194
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94136939783466	34.0	33.0	34.0	31.0	34.0
2	33.27778433775657	34.0	34.0	34.0	31.0	34.0
3	33.39969950773014	34.0	34.0	34.0	31.0	34.0
4	36.61928077297361	37.0	37.0	37.0	35.0	37.0
5	36.62386755538545	37.0	37.0	37.0	35.0	37.0
6	36.42862697834257	37.0	37.0	37.0	35.0	37.0
7	36.23854729495881	37.0	37.0	37.0	35.0	37.0
8	36.40149513226953	37.0	37.0	37.0	35.0	37.0
9	38.29291481573472	39.0	39.0	39.0	37.0	39.0
10	38.15804346144294	39.0	39.0	39.0	35.0	39.0
11	37.419962377390604	39.0	35.0	39.0	35.0	39.0
12	37.37547893500328	39.0	35.0	39.0	35.0	39.0
13	37.37955064597695	39.0	35.0	39.0	35.0	39.0
14	38.591200218243706	41.0	35.0	41.0	35.0	41.0
15	38.61495865177506	41.0	35.0	41.0	35.0	41.0
16	38.62519289730738	41.0	35.0	41.0	35.0	41.0
17	38.7253284852828	41.0	36.0	41.0	35.0	41.0
18	38.73502322911111	41.0	36.0	41.0	35.0	41.0
19	38.66402480486325	41.0	35.0	41.0	35.0	41.0
20	38.609693929486106	41.0	35.0	41.0	35.0	41.0
21	38.561468584713985	41.0	35.0	41.0	35.0	41.0
22	38.54300744715937	41.0	35.0	41.0	35.0	41.0
23	38.52666767102204	41.0	35.0	41.0	35.0	41.0
24	38.4777480995289	40.0	35.0	41.0	35.0	41.0
25	38.430829773979326	40.0	35.0	41.0	35.0	41.0
26	38.382392700236565	40.0	35.0	41.0	35.0	41.0
27	38.46542710212258	40.0	35.0	41.0	35.0	41.0
28	38.47622324376926	40.0	35.0	41.0	35.0	41.0
29	38.48382716401259	40.0	36.0	41.0	35.0	41.0
30	38.401092032883135	40.0	35.0	41.0	35.0	41.0
31	38.319120347561245	40.0	35.0	41.0	35.0	41.0
32	38.2671408852714	40.0	35.0	41.0	35.0	41.0
33	38.17925911147123	40.0	35.0	41.0	35.0	41.0
34	38.04626481593831	40.0	35.0	41.0	35.0	41.0
35	37.98879261554498	40.0	35.0	41.0	35.0	41.0
36	37.94933977206562	40.0	35.0	41.0	35.0	41.0
37	37.860845205763916	40.0	35.0	41.0	35.0	41.0
38	37.8563785388258	40.0	35.0	41.0	35.0	41.0
39	37.77579327109044	40.0	35.0	41.0	35.0	41.0
40	37.725530034975996	40.0	35.0	41.0	35.0	41.0
41	37.67628472660497	40.0	35.0	41.0	35.0	41.0
42	37.61984674079895	39.0	35.0	41.0	35.0	41.0
43	37.556578459834604	39.0	35.0	41.0	35.0	41.0
44	37.436969914127616	39.0	35.0	41.0	35.0	41.0
45	37.45517656974637	39.0	35.0	41.0	35.0	41.0
46	37.4180303505336	39.0	35.0	41.0	35.0	41.0
47	37.345537608358406	39.0	35.0	41.0	35.0	41.0
48	37.30090147680957	39.0	35.0	41.0	35.0	41.0
49	37.23867962556546	39.0	35.0	41.0	35.0	41.0
50	37.18311298590781	38.0	35.0	41.0	35.0	41.0
51	37.12393473861651	38.0	35.0	41.0	35.0	41.0
52	36.682819822717704	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	4.0
16	12.0
17	9.0
18	39.0
19	54.0
20	106.0
21	170.0
22	280.0
23	403.0
24	763.0
25	1329.0
26	1915.0
27	2147.0
28	2162.0
29	2235.0
30	2531.0
31	3077.0
32	4111.0
33	6269.0
34	23147.0
35	110271.0
36	16252.0
37	27005.0
38	47164.0
39	237694.0
40	2039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.190755587405384	21.26675000101793	23.270642556708754	22.271851854867933
2	26.445355602877886	24.39321327214909	26.7089988884229	22.45243223655012
3	27.029442542050596	24.208968350590602	26.03696299221896	22.72462611513984
4	25.910536366486564	26.81506695928696	23.094744642646287	24.17965203158019
5	23.914583647194387	34.7025818719284	21.222979108050993	20.159855372826215
6	85.49452965630687	2.490869188141549	8.519037284657385	3.495563870894188
7	84.97314706612866	2.463181553520605	6.470559493804892	6.093111886545845
8	74.0198373758637	4.455469732936478	13.454968912486716	8.069723978713094
9	52.237201594482016	15.92405444691914	15.709475278606824	16.12926867999202
10	42.44534745945594	17.9704963822848	25.238296884733934	14.345859273525328
11	39.253736812746084	15.676494419720111	25.415416312088503	19.654352455445302
12	19.57006803829037	31.559017414707835	30.137379528251568	18.73353501875023
13	14.478393465718229	34.57269429186838	31.428519078001766	19.52039316441162
14	13.260341127945374	20.598378644690285	47.31267075737896	18.828609469985384
15	15.028888789358177	16.65798034992284	47.594025985659435	20.71910487505955
16	15.580198455192857	19.510824643623497	29.88920874440649	35.01976815677716
17	18.390289783669996	21.80930548825922	35.37197115599946	24.42843357207132
18	23.44328310199229	26.64670171052578	27.86678990378547	22.04322528369646
19	25.53044214709463	22.62955166390469	25.442900361160763	26.397105827839916
20	19.791161944160557	31.340773706519215	26.77068530967398	22.09737903964625
21	19.797065925072374	23.84332870515519	25.712244042068917	30.647361327703514
22	18.005513096658348	30.322235206456106	23.508226892022297	28.164024804863253
23	18.436300117672445	21.855519407810355	36.96848902877478	22.739691445742416
24	18.7830063070803	21.167603838809107	34.88621603684084	25.163173817269758
25	15.175470384410234	28.330557783686285	31.165282963554112	25.32868886834937
26	18.32432806589657	27.049597511370255	27.897734907185352	26.728339515547827
27	20.38033852205035	25.124696148568592	35.62991404618134	18.865051283199712
28	18.397211692325232	23.133425896896135	31.618871566020758	26.850490844757875
29	19.880536000032574	25.5320708314841	29.733465799663676	24.85392736881965
30	28.689886277112503	24.095164028876574	21.47746104390526	25.73748865010566
31	28.52070668615659	23.265349332442987	26.585422460372072	21.62852152102835
32	25.89709972027346	24.92905043628383	24.0310345810413	25.142815262401413
33	20.251876040831117	25.21610605992744	30.414866631107056	24.117151268134382
34	20.48762810620651	23.941864110717965	30.67016290915606	24.90034487391947
35	26.860059365545997	25.292247055135036	26.121654580471258	21.726038998847706
36	23.749475767212143	29.82711515205805	24.123258834594886	22.30015024613493
37	19.968688542612494	34.382545389398075	24.519843483430172	21.128922584559255
38	21.157424561374935	31.10115351571884	22.892787778352343	24.848634144553884
39	26.945158124895663	24.67477208597825	24.485233940153993	23.894835848972097
40	19.046038835979267	21.419846333627852	29.00422236427969	30.529892466113186
41	22.743763156716085	25.03634002043999	24.524729536598574	27.695167286245354
42	22.940223211195576	20.640113682170387	28.476121450994924	27.943541655639116
43	24.325012113340147	24.724039788759637	28.65792334596921	22.29302475193101
44	17.745941522086998	28.580153666372148	27.63592389157848	26.03798091996238
45	21.960569550930998	27.824851280756686	26.565878247698464	23.64870092061385
46	23.735631949901666	21.77164216175279	28.61679906513516	25.87592682321038
47	22.59901383160218	19.764899408380394	31.83467224762517	25.801414512392252
48	22.045261139183296	17.96337088808088	29.1870421869974	30.804325785738424
49	20.2999222303204	24.076026987300335	30.554729903052564	25.0693208793267
50	20.301958085807236	25.170910068119724	27.75033896993856	26.77679287613448
51	19.731714963945	20.791174159293476	30.95029662414443	28.526814252617093
52	22.151329210047354	18.97885560491374	34.2608012312854	24.609013953753507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	184.0
2	273.0
3	1109.5
4	1946.0
5	1319.0
6	692.0
7	744.5
8	797.0
9	866.5
10	936.0
11	883.5
12	831.0
13	796.5
14	692.0
15	622.0
16	604.0
17	586.0
18	640.5
19	695.0
20	701.5
21	708.0
22	704.0
23	700.0
24	815.5
25	931.0
26	1110.5
27	1290.0
28	1652.5
29	2015.0
30	2298.0
31	2581.0
32	2771.0
33	2961.0
34	3783.5
35	4606.0
36	5196.5
37	5787.0
38	6843.0
39	10212.0
40	12525.0
41	19179.0
42	25833.0
43	37107.0
44	48381.0
45	59107.0
46	69833.0
47	68623.5
48	67414.0
49	58734.5
50	50055.0
51	43404.0
52	36753.0
53	32428.0
54	28103.0
55	24942.0
56	21781.0
57	20283.0
58	18785.0
59	18360.5
60	17936.0
61	16927.5
62	15919.0
63	14542.5
64	11022.0
65	8878.0
66	7547.0
67	6216.0
68	4997.0
69	3778.0
70	3378.5
71	2979.0
72	2444.0
73	1909.0
74	1717.5
75	1526.0
76	1259.0
77	992.0
78	696.5
79	401.0
80	299.5
81	198.0
82	123.0
83	48.0
84	58.0
85	68.0
86	35.0
87	2.0
88	1.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	491194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.84270244261449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.63224220296294	20.372247447147373
2	11.397226254762272	6.574536110745235
3	4.073620916359038	3.524827078636629
4	1.9783409732416128	2.2824280008496083
5	1.1709045986654314	1.6886026463997987
6	0.8501334194706963	1.47120871525894
7	0.6513748281668558	1.3151187243198033
8	0.5276354554794468	1.2174745952453638
9	0.49632436137133534	1.2883802283048682
>10	7.816575833284971	47.245160004643324
>50	0.31855064625820156	6.127078574252488
>100	0.07999160672705949	4.2172284298438845
>500	0.0035394516250911285	0.753200468540019
>1k	0.0035394516250911285	1.9225089758126372
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	2855	0.581236741491142	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	2212	0.4503312336877079	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1920	0.39088425347215155	No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTTG	1360	0.2768763462094407	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1069	0.21763295154256768	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	993	0.20216044984262835	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	710	0.1445457395652227	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	710	0.1445457395652227	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	675	0.13742024536130326	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	601	0.12235491475873075	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	496	0.10097843214697248	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	494	0.10057126104960565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4584746556350444	0.0
2	0.0	0.0	0.0	1.6950532783380905	0.0
3	0.0	0.0	0.0	2.6050806809529434	0.0
4	0.0	0.0	0.0	3.2052508784716425	0.0
5	0.0	0.0	0.0	6.379353167994723	0.0
6	2.0358554868341226E-4	0.0	0.0	7.001714190319914	0.0
7	2.0358554868341226E-4	0.0	0.0	10.189456711604784	0.0
8	2.0358554868341226E-4	0.0	0.0	13.405904795254013	0.0
9	2.0358554868341226E-4	0.0	0.0	16.643118604868953	0.0
10	2.0358554868341226E-4	0.0	0.0	17.967035427957182	0.0
11	2.0358554868341226E-4	0.0	0.0	19.254103266733715	0.0
12	2.0358554868341226E-4	0.0	0.0	20.384206647475335	0.0
13	2.0358554868341226E-4	0.0	0.0	20.79972475233818	0.0
14	2.0358554868341226E-4	0.0	0.0	21.087798303725208	0.0
15	2.0358554868341226E-4	0.0	0.0	22.15886187534864	0.0
16	2.0358554868341226E-4	0.0	0.0	23.20345932564323	0.0
17	2.0358554868341226E-4	0.0	0.0	24.724446959857	0.0
18	2.0358554868341226E-4	0.0	0.0	25.368388050342634	0.0
19	2.0358554868341226E-4	0.0	0.0	26.320761247083638	0.0
20	2.0358554868341226E-4	0.0	0.0	27.261122896452317	0.0
21	2.0358554868341226E-4	0.0	0.0	28.01296432774016	0.0
22	4.071710973668245E-4	0.0	0.0	28.86008379581184	0.0
23	4.071710973668245E-4	0.0	0.0	29.454146426870036	0.0
24	4.071710973668245E-4	0.0	0.0	29.93949437493129	0.0
25	4.071710973668245E-4	0.0	0.0	30.454565813100324	0.0
26	4.071710973668245E-4	0.0	0.0	30.92098030513402	0.0
27	4.071710973668245E-4	0.0	0.0	31.411621477461043	0.0
28	4.071710973668245E-4	0.0	0.0	31.857473829077716	0.0
29	4.071710973668245E-4	0.0	0.0	32.45744044104773	0.0
30	4.071710973668245E-4	0.0	0.0	33.05964649405327	0.0
31	4.071710973668245E-4	0.0	0.0	33.537054605715866	0.0
32	4.071710973668245E-4	0.0	0.0	34.03787505547706	0.0
33	4.071710973668245E-4	0.0	0.0	34.488409874713454	0.0
34	4.071710973668245E-4	0.0	0.0	35.12441112880043	0.0
35	4.071710973668245E-4	0.0	0.0	35.59163996302886	0.0
36	4.071710973668245E-4	0.0	0.0	36.06945524578883	0.0
37	4.071710973668245E-4	0.0	0.0	36.54523467306197	0.0
38	4.071710973668245E-4	0.0	0.0	37.007984625219365	0.0
39	4.071710973668245E-4	0.0	0.0	37.50167958077664	0.0
40	4.071710973668245E-4	0.0	0.0	38.04301355472583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACGG	35	1.0179792E-7	46.000004	1
GTGCACG	20	6.3079066E-4	46.0	20
GGTACAC	20	6.3079066E-4	46.0	7
AGATCGT	20	6.3079066E-4	46.0	10
GTACAGG	120	0.0	46.0	1
AAATACG	20	6.3079066E-4	46.0	27
CACGGGT	25	3.4142773E-5	46.0	3
TACGGGA	65	0.0	46.0	3
ACGGGTA	45	3.092282E-10	46.0	4
ACGGGCC	20	6.3079066E-4	46.0	4
AATGCGG	65	0.0	46.0	1
ACCTAGG	45	3.092282E-10	46.0	1
CTAAACG	20	6.3079066E-4	46.0	45
GGTATGT	20	6.3079066E-4	46.0	7
ACGCTAA	20	6.3079066E-4	46.0	34
ATGCGCT	20	6.3079066E-4	46.0	2
TTCGGAC	25	3.4142773E-5	46.0	13
GGCATAG	40	5.6006684E-9	46.0	7
ATAACGG	20	6.3079066E-4	46.0	1
ACGTAGG	55	1.8189894E-12	46.0	1
>>END_MODULE