FastQCFastQC Report
Thu 26 May 2016
SRR1545010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545010_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences559957
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT22780.40681695201595836TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT17410.3109167311061385TruSeq Adapter, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT16100.2875220775881005Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.158762190668212No Hit
ACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC7020.12536676923406617Illumina Single End Adapter 1 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT6960.12429525838591178No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT6920.1235809178204755TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC6470.11554458645931741TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCA206.3086674E-446.00000429
ACCGGGT206.3086674E-446.0000043
CTCGTTA206.3086674E-446.00000444
TAGACTA206.3086674E-446.00000419
CGGCCTT206.3086674E-446.0000045
TCCGAGT206.3086674E-446.00000415
TCCGAAT206.3086674E-446.00000412
TTAGATC206.3086674E-446.00000412
TTGCTAG206.3086674E-446.00000435
TAGGTAT206.3086674E-446.00000416
CTATACG206.3086674E-446.00000413
ATTCGAA206.3086674E-446.00000441
CGACAAT405.6024874E-946.00000419
TGCGGAA206.3086674E-446.0000043
ATATCGG206.3086674E-446.00000439
TCGGAGA206.3086674E-446.00000429
AAGTTGG405.6024874E-946.0000041
TTGACAC206.3086674E-446.00000414
GATATCG206.3086674E-446.00000438
GTCCGAA206.3086674E-446.00000411
Produced by FastQC (version 0.11.5)