##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545010_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	559957
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91934380675659	34.0	33.0	34.0	31.0	34.0
2	33.21638625823054	34.0	33.0	34.0	31.0	34.0
3	33.265656112880094	34.0	34.0	34.0	31.0	34.0
4	36.55284066455103	37.0	37.0	37.0	35.0	37.0
5	36.54053436245997	37.0	37.0	37.0	35.0	37.0
6	36.65782015404754	37.0	37.0	37.0	35.0	37.0
7	36.470141457290474	37.0	37.0	37.0	35.0	37.0
8	36.523727714806675	37.0	37.0	37.0	35.0	37.0
9	38.320926428279314	39.0	39.0	39.0	37.0	39.0
10	38.10217213107435	39.0	39.0	39.0	35.0	39.0
11	37.22618879663974	39.0	35.0	39.0	35.0	39.0
12	37.10666354737953	39.0	35.0	39.0	35.0	39.0
13	37.09466619758303	39.0	35.0	39.0	35.0	39.0
14	38.3238677255575	40.0	35.0	41.0	35.0	41.0
15	38.404500702732534	40.0	35.0	41.0	35.0	41.0
16	38.418950026519894	40.0	35.0	41.0	35.0	41.0
17	38.523804506417456	40.0	36.0	41.0	35.0	41.0
18	38.513303700105546	40.0	36.0	41.0	35.0	41.0
19	38.39836630312685	40.0	35.0	41.0	35.0	41.0
20	38.30507699698369	40.0	35.0	41.0	35.0	41.0
21	38.255058870591846	40.0	35.0	41.0	35.0	41.0
22	38.23157492450313	40.0	35.0	41.0	35.0	41.0
23	38.21843105809911	40.0	35.0	41.0	35.0	41.0
24	38.16130345722975	40.0	35.0	41.0	35.0	41.0
25	38.12825806267267	40.0	35.0	41.0	35.0	41.0
26	38.09840041288885	40.0	35.0	41.0	35.0	41.0
27	38.12317910125242	40.0	35.0	41.0	35.0	41.0
28	38.1348978582284	40.0	35.0	41.0	35.0	41.0
29	38.161707059649224	40.0	35.0	41.0	35.0	41.0
30	38.05462026548467	40.0	35.0	41.0	35.0	41.0
31	37.998524886732376	40.0	35.0	41.0	35.0	41.0
32	37.91826693835419	40.0	35.0	41.0	35.0	41.0
33	37.863360936643346	40.0	35.0	41.0	35.0	41.0
34	37.786496105950995	40.0	35.0	41.0	35.0	41.0
35	37.726898672576645	40.0	35.0	41.0	35.0	41.0
36	37.68351141248346	40.0	35.0	41.0	35.0	41.0
37	37.64346726623651	40.0	35.0	41.0	35.0	41.0
38	37.591429341895896	40.0	35.0	41.0	35.0	41.0
39	37.49726496856009	40.0	35.0	41.0	35.0	41.0
40	37.427973933712764	39.0	35.0	41.0	34.0	41.0
41	37.41740526504714	39.0	35.0	41.0	34.0	41.0
42	37.322424757615316	39.0	35.0	41.0	35.0	41.0
43	37.235201988724135	39.0	35.0	41.0	34.0	41.0
44	37.167975398110926	39.0	35.0	41.0	34.0	41.0
45	37.13787665838627	39.0	35.0	41.0	34.0	41.0
46	37.16056768644735	39.0	35.0	41.0	34.0	41.0
47	37.128449148773925	38.0	35.0	41.0	35.0	41.0
48	37.167857531917626	38.0	35.0	41.0	35.0	41.0
49	37.08038831553137	38.0	35.0	41.0	34.0	41.0
50	36.99299410490448	38.0	35.0	41.0	34.0	41.0
51	36.92974996294358	38.0	35.0	41.0	34.0	41.0
52	36.73963357900696	37.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	6.0
14	8.0
15	6.0
16	4.0
17	25.0
18	59.0
19	81.0
20	159.0
21	239.0
22	409.0
23	679.0
24	1118.0
25	1915.0
26	2364.0
27	2860.0
28	2830.0
29	3095.0
30	3791.0
31	4596.0
32	6087.0
33	9115.0
34	27718.0
35	139392.0
36	17839.0
37	26581.0
38	48883.0
39	256523.0
40	3573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.43238855840716	22.74906823202496	24.043631921736846	19.774911287831028
2	26.277732040138797	23.80914963113239	27.00528790603564	22.90783042269317
3	25.167289631168106	25.504101207771313	26.534358888271782	22.794250272788805
4	26.10075416505196	26.909387685125825	23.255357107777918	23.7345010420443
5	22.943011695540907	36.69799645329909	21.03429370469518	19.324698146464815
6	87.16026409170703	2.8270027877140564	7.148048868038082	2.8646842525408203
7	86.60450713179763	2.760390529987124	5.265582892972139	5.369519445243117
8	76.399437813975	4.714469146738053	11.79251263936338	7.093580399923566
9	53.47910643138669	16.39768767958968	14.812030209462513	15.31117567956111
10	45.49045730297148	18.429986588255883	24.439198009847185	11.640358098925454
11	43.029375469902156	16.117844763080022	23.85129572449313	17.001484042524694
12	20.091899913743376	36.55084229681922	27.380138117748327	15.977119671689078
13	13.272090535523262	39.48142446652154	28.420217981023544	18.82626701693166
14	11.714470932589467	21.45807624514025	49.57755684811512	17.24989597415516
15	13.166725302121412	16.374114440930285	49.91133247731522	20.547827779633078
16	12.99081893788273	19.53489285784444	29.27028325389271	38.20400495038012
17	17.811546243729428	22.192596931550103	36.717640818848594	23.27821600587188
18	25.3531967633229	26.552574572690403	27.01296706711408	21.081261596872615
19	27.135119303803684	22.69888580730306	25.671078314942044	24.494916573951215
20	18.25765192684438	32.443919800984716	27.08261527224412	22.21581299992678
21	19.568645449561306	24.774580905319514	24.354905823125705	31.301867821993472
22	16.496802433043968	32.69161739204975	22.11794834246201	28.69363183244428
23	16.98112533640976	22.39189794930682	38.94120441391035	21.685772300373067
24	16.656457549418974	22.65691829908368	36.69210314363424	23.994521007863103
25	14.184482022726744	31.104888411074423	30.96577058595571	23.744858980243126
26	17.236859258835945	30.721108942293785	27.31459737086955	24.727434428000723
27	17.978880521182877	26.874384997419444	38.194004182464006	16.95273029893367
28	15.756209851827908	23.57734611764832	33.32916634670162	27.337277683822155
29	17.42187346528394	28.11055134590692	30.94951933809203	23.518055850717108
30	26.24647964040096	24.260434283346758	25.017456697567848	24.475629378684435
31	28.05661863321648	21.902396076841615	25.923419119682407	24.1175661702595
32	23.425191577210395	24.22382432936815	30.078381018542494	22.272603074878962
33	15.746030498770441	22.671205110392407	31.020953394635658	30.561810996201494
34	16.023194638159715	20.792489423295002	36.25617681357676	26.928139124968524
35	28.75595804677859	18.843946945926206	27.442642917224003	24.957452090071204
36	18.6607185908918	24.532062283353902	32.11889484371122	24.688324282043087
37	19.90849297356761	26.407027682482763	28.925613931069705	24.758865412879917
38	19.4434572654686	25.52392415846217	27.69998410592242	27.332634470146814
39	26.550074380711376	19.549536839435884	27.153156403080953	26.74723237677179
40	14.802208026687763	18.528029830862014	31.815478688542154	34.85428345390807
41	20.760879853274446	22.097768221488437	26.819916529304926	30.321435395932188
42	21.016792360841993	20.295844145175433	29.795502154629734	28.891861339352843
43	21.157160281950222	25.26979750230821	28.995440721341105	24.577601494400465
44	14.91400232517854	30.445373484035382	29.135272887025256	25.505351303760825
45	21.178054743489234	27.27048684095386	28.10787256878653	23.443585846770375
46	22.21045544568601	20.601581907182158	30.432872524140247	26.755090122991586
47	22.56494695128376	17.8819087894249	32.930564311188185	26.62257994810316
48	19.915993549504694	16.48055118518029	30.794686020533717	32.808769244781296
49	20.198336657993384	23.957911053884494	31.098459345985496	24.74529294213663
50	18.7069721425038	25.480527969111915	27.756059840309167	28.05644004807512
51	18.874663590239965	20.290308005793303	32.95645915668525	27.878569247281487
52	23.112131824407946	17.638675826893852	34.884642927939105	24.364549420759094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	152.0
2	204.0
3	918.0
4	1632.0
5	1139.0
6	646.0
7	755.0
8	864.0
9	902.5
10	941.0
11	909.5
12	878.0
13	860.5
14	812.0
15	781.0
16	704.0
17	627.0
18	672.5
19	718.0
20	704.0
21	690.0
22	885.0
23	1080.0
24	1062.0
25	1044.0
26	1358.5
27	1673.0
28	2536.0
29	3399.0
30	3499.0
31	3599.0
32	4264.0
33	4929.0
34	5446.0
35	5963.0
36	7429.5
37	8896.0
38	10427.5
39	13794.5
40	15630.0
41	23232.5
42	30835.0
43	42443.5
44	54052.0
45	61606.0
46	69160.0
47	71693.0
48	74226.0
49	72178.5
50	70131.0
51	62428.0
52	54725.0
53	45889.5
54	37054.0
55	30608.0
56	24162.0
57	21132.5
58	18103.0
59	17213.0
60	16323.0
61	15388.5
62	14454.0
63	12654.0
64	8762.5
65	6671.0
66	5544.5
67	4418.0
68	3624.0
69	2830.0
70	2338.0
71	1846.0
72	1434.5
73	1023.0
74	913.0
75	803.0
76	676.5
77	550.0
78	457.5
79	365.0
80	269.5
81	174.0
82	124.0
83	74.0
84	51.0
85	28.0
86	14.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	559957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.565979870956404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.63327761639553	17.106096962730916
2	12.156477886047206	5.972715821007247
3	4.679491670008564	3.4486889451521554
4	2.290733158531922	2.2509641864891035
5	1.2800450231787373	1.5722780136663388
6	0.8387017228056138	1.23621177841075
7	0.6007856500791187	1.0331221730622158
8	0.44761689184455056	0.8796918043962638
9	0.39149694512460775	0.8655755466124836
>10	6.562312740465368	43.070373537379844
>50	0.9706287176996675	15.374126311005758
>100	0.14042829274065655	5.250782850208171
>500	0.005820861875260375	0.9332222651462712
>1k	0.002182823203222641	1.0061498047324957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	2278	0.40681695201595836	TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	1741	0.3109167311061385	TruSeq Adapter, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT	1610	0.2875220775881005	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	889	0.158762190668212	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	702	0.12536676923406617	Illumina Single End Adapter 1 (95% over 21bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	696	0.12429525838591178	No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	692	0.1235809178204755	TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	647	0.11554458645931741	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29127236555664093	0.0
2	0.0	0.0	0.0	1.2367021039115504	0.0
3	0.0	0.0	0.0	2.0071184037345726	0.0
4	0.0	0.0	0.0	2.469118164430483	0.0
5	0.0	0.0	0.0	5.1953989324180245	0.0
6	0.0	0.0	0.0	5.715796034338351	0.0
7	0.0	0.0	0.0	8.64780688517154	0.0
8	0.0	0.0	0.0	11.689290427657838	0.0
9	0.0	0.0	0.0	14.878285296906727	0.0
10	0.0	0.0	0.0	16.025873415280103	0.0
11	0.0	0.0	0.0	17.13595865396807	0.0
12	0.0	0.0	0.0	18.076923763789004	0.0
13	0.0	0.0	0.0	18.414449680957645	0.0
14	0.0	0.0	0.0	18.674648231917807	0.0
15	0.0	0.0	0.0	19.64400837921483	0.0
16	0.0	0.0	0.0	20.679087858531993	0.0
17	0.0	0.0	0.0	22.34635873826026	0.0
18	0.0	0.0	0.0	22.92943922479762	0.0
19	0.0	0.0	0.0	23.721285741583728	0.0
20	0.0	0.0	0.0	24.554206840882426	0.0
21	0.0	0.0	0.0	25.253724839585896	0.0
22	0.0	0.0	0.0	26.11021917754399	0.0
23	0.0	0.0	0.0	26.666869063160206	0.0
24	0.0	0.0	0.0	27.13029750498699	0.0
25	0.0	0.0	0.0	27.605155395860752	0.0
26	0.0	0.0	0.0	28.05268976010658	0.0
27	0.0	0.0	0.0	28.514332350519773	0.0
28	0.0	0.0	0.0	28.941329423509305	0.0
29	0.0	0.0	0.0	29.5408397430517	0.0
30	0.0	0.0	0.0	30.099454065222865	0.0
31	0.0	0.0	0.0	30.561989581342853	0.0
32	0.0	0.0	0.0	31.0050593170547	0.0
33	0.0	0.0	0.0	31.41026900279843	0.0
34	0.0	0.0	0.0	32.053711267115155	0.0
35	0.0	0.0	0.0	32.4928521297171	0.0
36	0.0	0.0	0.0	32.953066038999424	0.0
37	0.0	0.0	0.0	33.390063879905064	0.0
38	0.0	0.0	0.0	33.76419975105231	0.0
39	0.0	0.0	0.0	34.245129536732286	0.0
40	0.0	0.0	0.0	34.70284325403558	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCA	20	6.3086674E-4	46.000004	29
ACCGGGT	20	6.3086674E-4	46.000004	3
CTCGTTA	20	6.3086674E-4	46.000004	44
TAGACTA	20	6.3086674E-4	46.000004	19
CGGCCTT	20	6.3086674E-4	46.000004	5
TCCGAGT	20	6.3086674E-4	46.000004	15
TCCGAAT	20	6.3086674E-4	46.000004	12
TTAGATC	20	6.3086674E-4	46.000004	12
TTGCTAG	20	6.3086674E-4	46.000004	35
TAGGTAT	20	6.3086674E-4	46.000004	16
CTATACG	20	6.3086674E-4	46.000004	13
ATTCGAA	20	6.3086674E-4	46.000004	41
CGACAAT	40	5.6024874E-9	46.000004	19
TGCGGAA	20	6.3086674E-4	46.000004	3
ATATCGG	20	6.3086674E-4	46.000004	39
TCGGAGA	20	6.3086674E-4	46.000004	29
AAGTTGG	40	5.6024874E-9	46.000004	1
TTGACAC	20	6.3086674E-4	46.000004	14
GATATCG	20	6.3086674E-4	46.000004	38
GTCCGAA	20	6.3086674E-4	46.000004	11
>>END_MODULE