FastQCFastQC Report
Thu 26 May 2016
SRR1545009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545009_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences287942
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT13530.46988629654583214TruSeq Adapter, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT9650.33513693729987293Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT9450.3281910940397719TruSeq Adapter, Index 23 (96% over 25bp)
TGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTTG6090.21150092727007525TruSeq Adapter, Index 23 (96% over 27bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG5680.19726194858686819No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.1701731598724743No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT3780.13127643761590874TruSeq Adapter, Index 23 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC3720.12919268463787847Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC3570.12398330219280272TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCG206.3035364E-446.030
CGGGTGG253.4107343E-546.05
CGGGTAA253.4107343E-546.05
GTCGAGG253.4107343E-546.01
GTCGAAT301.8565115E-646.029
TACCAGG405.5861165E-946.01
TTTACGG206.3035364E-446.01
CAACGCA750.046.016
CATGCGG501.6370905E-1146.01
TTAGCGG206.3035364E-446.01
GCGAAGA253.4107343E-546.035
ACGCAAG800.046.018
GCGCGCG405.5861165E-946.015
GGGTCAA206.3035364E-446.06
ATAATGG301.8565115E-646.01
AGCTTAT750.046.023
AAGACGG253.4107343E-546.01
CTCTTGA206.3035364E-446.020
ATTCGAA301.8565115E-646.041
TACGGGA206.3035364E-446.03
Produced by FastQC (version 0.11.5)