FastQCFastQC Report
Thu 26 May 2016
SRR1545008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545008_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1150432
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC52870.45956649328252347No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT52240.4540902895607911No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC36400.3164028817000918No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT35310.3069281800228088No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT34010.29562807710494843Illumina Single End Adapter 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26820.23312981558232035No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA25290.21983046368668466No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19090.16593766515535033No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16310.14177282968484883No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA15260.13264582348196155No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14860.1291688687380045No Hit
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC13650.11865108063753443No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT12760.11091485633222997No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC11830.10283093655252983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG351.01957994E-746.00000439
CGACGGT1500.046.027
GCGAACG206.311458E-446.024
TAGGTCG206.311458E-446.041
ATTCGGC301.8614046E-646.016
TACGGGT1200.046.03
TTATCGG253.417158E-546.01
TACTCGC253.417158E-546.044
CCGAATA206.311458E-446.010
GCGTATA206.311458E-446.030
ATAGCGG1850.044.7567561
CACGACG1750.044.68571525
CGGGATA1650.044.6060645
CGGTCTA1550.044.5161330
ACGGGAT2050.043.7560964
CGGGTAT2850.043.578955
TACGCGG1850.043.5135151
CTAGGGT1500.042.933333
CGTTTTT17150.042.9154551
CATGCGG2150.042.7906951
Produced by FastQC (version 0.11.5)