##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545008_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1150432
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8948716656004	34.0	33.0	34.0	31.0	34.0
2	33.202585637395345	34.0	33.0	34.0	31.0	34.0
3	33.27822070317933	34.0	34.0	34.0	31.0	34.0
4	36.54084639509332	37.0	37.0	37.0	35.0	37.0
5	36.54110629746043	37.0	37.0	37.0	35.0	37.0
6	36.639431100664794	37.0	37.0	37.0	35.0	37.0
7	36.511804261355735	37.0	37.0	37.0	35.0	37.0
8	36.53853856638202	37.0	37.0	37.0	35.0	37.0
9	38.294568475146725	39.0	39.0	39.0	37.0	39.0
10	38.164163548997244	39.0	39.0	39.0	35.0	39.0
11	37.63381668799199	39.0	37.0	39.0	35.0	39.0
12	37.54926236405107	39.0	37.0	39.0	35.0	39.0
13	37.49194650357431	39.0	37.0	39.0	35.0	39.0
14	38.876144787349446	41.0	37.0	41.0	35.0	41.0
15	38.947245034908626	41.0	37.0	41.0	35.0	41.0
16	38.94866363244416	41.0	37.0	41.0	35.0	41.0
17	38.9994723721176	41.0	37.0	41.0	35.0	41.0
18	38.977944806820396	41.0	37.0	41.0	35.0	41.0
19	38.907274832410785	41.0	37.0	41.0	35.0	41.0
20	38.84147085616534	41.0	36.0	41.0	35.0	41.0
21	38.805367027342776	41.0	36.0	41.0	35.0	41.0
22	38.786868758866234	41.0	35.0	41.0	35.0	41.0
23	38.768683416316655	41.0	35.0	41.0	35.0	41.0
24	38.687869426441544	41.0	35.0	41.0	35.0	41.0
25	38.669884008789744	41.0	35.0	41.0	35.0	41.0
26	38.6053082668076	41.0	35.0	41.0	35.0	41.0
27	38.58128250952686	41.0	36.0	41.0	35.0	41.0
28	38.61205095129482	41.0	36.0	41.0	35.0	41.0
29	38.63848102278101	41.0	36.0	41.0	35.0	41.0
30	38.54173041083697	40.0	36.0	41.0	35.0	41.0
31	38.513116811771575	41.0	35.0	41.0	35.0	41.0
32	38.441455905259936	40.0	35.0	41.0	35.0	41.0
33	38.365932971266446	40.0	35.0	41.0	35.0	41.0
34	38.278648368612835	40.0	35.0	41.0	35.0	41.0
35	38.21397092570443	40.0	35.0	41.0	35.0	41.0
36	38.16374979138271	40.0	35.0	41.0	35.0	41.0
37	38.10719799171094	40.0	35.0	41.0	35.0	41.0
38	38.029481099274015	40.0	35.0	41.0	35.0	41.0
39	37.95403205056883	40.0	35.0	41.0	35.0	41.0
40	37.81461833467775	40.0	35.0	41.0	35.0	41.0
41	37.78706607604796	40.0	35.0	41.0	35.0	41.0
42	37.68841009290423	40.0	35.0	41.0	35.0	41.0
43	37.59769025896359	40.0	35.0	41.0	34.0	41.0
44	37.52891957108286	40.0	35.0	41.0	34.0	41.0
45	37.48371307474062	39.0	35.0	41.0	34.0	41.0
46	37.48464229089594	39.0	35.0	41.0	34.0	41.0
47	37.38579246752524	39.0	35.0	41.0	34.0	41.0
48	37.37343363188785	39.0	35.0	41.0	34.0	41.0
49	37.29686065756168	39.0	35.0	41.0	34.0	41.0
50	37.18274526438764	39.0	35.0	41.0	34.0	41.0
51	37.04328547884621	39.0	35.0	41.0	34.0	41.0
52	36.72605768963311	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	7.0
13	3.0
14	9.0
15	21.0
16	17.0
17	37.0
18	81.0
19	156.0
20	277.0
21	455.0
22	740.0
23	1292.0
24	2035.0
25	3572.0
26	5092.0
27	5900.0
28	6114.0
29	6575.0
30	7941.0
31	9484.0
32	12400.0
33	17536.0
34	52458.0
35	186045.0
36	40845.0
37	64561.0
38	122491.0
39	599389.0
40	4897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.38902255848238	22.039546883257767	23.959434369002253	20.6119961892576
2	27.243244276932494	24.641873661372422	26.365487051820537	21.74939500987455
3	28.3111909265389	24.27844496676031	25.130820422241385	22.2795436844594
4	25.89409891240856	26.731523462490614	22.549789991933462	24.824587633167365
5	23.459100581346835	36.40241231120136	20.16998831743206	19.968498790019748
6	87.70661803565964	2.476461016383411	7.189560095685795	2.627360852271147
7	88.11281327362242	2.1950884537286863	5.3746766432088116	4.317421629440071
8	78.41454340630303	4.132186865455759	10.742051681455314	6.711218046785904
9	44.25876540290952	21.3731015827098	16.79647297710773	17.571660037272956
10	37.22749367194236	20.906928875413758	24.900124474979833	16.965452977664043
11	36.999926983950374	17.869026591749883	24.633702817724124	20.497343606575615
12	23.537332063085866	27.395274123112014	29.32159397513282	19.7457998386693
13	17.583220911796612	33.13224945064115	27.522530666740842	21.761998970821395
14	15.817970988289618	25.19001557675725	38.632531083975415	20.35948235097772
15	17.249433256376733	18.40917151122361	42.777930377458205	21.563464854941447
16	18.470365914717256	21.74365803454702	26.975605685516395	32.81037036521933
17	19.88670342966816	24.744269978581958	30.68577716892437	24.68324942282551
18	23.733345386776445	28.5780472031376	26.431896887430113	21.256710522655837
19	26.262743039136605	23.0974972879753	25.01938402269756	25.620375650190535
20	22.221826235709717	28.88280228644544	27.172401324024364	21.72297015382048
21	20.5632318989736	23.62060512920364	26.292297154460236	29.52386581736252
22	18.840661594948678	29.69241119857584	24.38623056382298	27.0806966426525
23	19.678955383716726	23.17529415037134	34.54102458902395	22.604725876887986
24	22.487465578148036	21.81580484548413	30.98149216989792	24.715237406469917
25	18.80832591582988	28.540496091902867	27.54791243637173	25.103265555895526
26	19.32326291340992	27.898911017774193	28.084145781758508	24.693680287057383
27	21.70193457761954	25.589517676837918	32.499878306583966	20.20866943895858
28	18.30173360963534	26.902763483630498	28.43853439403633	26.356968512697836
29	23.329149397791436	25.702518706016527	27.098429111846677	23.86990278434536
30	25.566569775527803	27.062529554115322	22.635410002503406	24.735490667853465
31	26.406428193930626	26.99064351478401	22.17923354009624	24.423694751189117
32	23.755250201663376	30.78208881533198	22.21974006286334	23.2429209201413
33	19.077268365274957	27.428479040916802	24.34650635587327	29.147746237934967
34	19.64540277043754	28.65593098940224	25.149335206252953	26.549331033907265
35	25.021296347806736	27.70063767350004	22.594468860393313	24.683597118299907
36	20.592090623348447	31.55197351951267	23.230403883063058	24.625531974075827
37	19.808124252454732	31.44723025785096	24.458811994103087	24.285833495591223
38	17.977681427498542	30.61354343411866	23.72960766042669	27.679167477956106
39	24.617448054296126	26.129575672443046	26.481095797057108	22.77188047620372
40	17.0821917331924	24.685770215014884	30.79721356846819	27.434824483324526
41	21.326075769797782	28.895927790603874	23.169035631832216	26.608960807766124
42	21.197254596534172	24.925071625267726	26.291427915774246	27.58624586242385
43	22.65314247169759	27.344075964507248	26.379916414007955	23.622865149787213
44	20.373650941559347	29.050391505104166	24.66490848655114	25.911049066785345
45	22.397760145753942	27.103036076882425	24.355894133681957	26.14330964368168
46	23.500215571194126	26.383219521014716	25.187407860699285	24.929157047091874
47	20.632075602903953	25.991888264582347	28.42723429111847	24.948801841395234
48	19.501630691774917	23.69970584962866	26.35462156824567	30.444041890350753
49	20.228661928736337	25.811695084976773	29.07377402575728	24.88586896052961
50	19.213564991238073	26.85234763984312	27.329820450057024	26.604266918861786
51	20.33123209368307	23.155301660593587	27.63501015270785	28.878456093015494
52	21.001675892186586	21.838318127451252	31.141779783594338	26.018226196767824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	289.5
2	468.0
3	2114.0
4	3760.0
5	2597.5
6	1435.0
7	1542.5
8	1650.0
9	1699.5
10	1749.0
11	1702.0
12	1655.0
13	1555.5
14	1419.5
15	1383.0
16	1366.5
17	1350.0
18	1198.5
19	1047.0
20	1131.5
21	1216.0
22	1407.5
23	1599.0
24	2035.5
25	2472.0
26	2813.5
27	3155.0
28	4683.0
29	6211.0
30	6856.5
31	7502.0
32	9199.0
33	10896.0
34	12223.5
35	13551.0
36	15755.5
37	17960.0
38	21248.0
39	29089.0
40	33642.0
41	44666.5
42	55691.0
43	76917.0
44	98143.0
45	116859.0
46	135575.0
47	138110.5
48	140646.0
49	132266.0
50	123886.0
51	110514.0
52	97142.0
53	83745.0
54	70348.0
55	63346.0
56	56344.0
57	52425.5
58	48507.0
59	47437.0
60	46367.0
61	43812.5
62	41258.0
63	39150.5
64	30242.5
65	23442.0
66	18998.5
67	14555.0
68	10954.0
69	7353.0
70	6454.0
71	5555.0
72	4676.0
73	3797.0
74	3331.0
75	2865.0
76	2288.0
77	1711.0
78	1240.5
79	770.0
80	543.5
81	317.0
82	271.0
83	225.0
84	127.5
85	30.0
86	41.5
87	53.0
88	26.5
89	1.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1150432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.29391546409028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.71092771125166	16.692574692763664
2	12.426012822934952	6.037530101521671
3	5.061921429627132	3.689216738916857
4	2.4640510478801283	2.394457914256116
5	1.4161830413798406	1.7202315544480058
6	0.942584021179431	1.3739433917001225
7	0.6574942418409605	1.1181176670587296
8	0.49250993174058444	0.9571995717544506
9	0.4095860213064213	0.8955403359202151
>10	6.374617036147303	41.02833237120237
>50	0.8861857676545339	13.455880734976406
>100	0.14536455785935393	5.976562435382704
>500	0.006101722181749323	1.0903099851477982
>1k	0.005742797347528774	2.653575933142818
>5k	7.178496684410967E-4	0.916526571808102
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5287	0.45956649328252347	No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	5224	0.4540902895607911	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3640	0.3164028817000918	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	3531	0.3069281800228088	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT	3401	0.29562807710494843	Illumina Single End Adapter 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2682	0.23312981558232035	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2529	0.21983046368668466	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1909	0.16593766515535033	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1631	0.14177282968484883	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	1526	0.13264582348196155	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1486	0.1291688687380045	No Hit
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1365	0.11865108063753443	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	1276	0.11091485633222997	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1183	0.10283093655252983	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3018865956440711	0.0
2	0.0	0.0	0.0	1.275260076214848	0.0
3	8.692386859892631E-5	0.0	0.0	1.9941204695279686	0.0
4	8.692386859892631E-5	0.0	0.0	2.445689966899391	0.0
5	8.692386859892631E-5	0.0	0.0	5.0796570331840565	0.0
6	8.692386859892631E-5	0.0	0.0	5.54078815610136	0.0
7	8.692386859892631E-5	0.0	0.0	7.641564212400211	0.0
8	8.692386859892631E-5	0.0	0.0	9.843258880142416	0.0
9	8.692386859892631E-5	0.0	0.0	11.74141539873717	0.0
10	8.692386859892631E-5	0.0	0.0	12.822400628633417	0.0
11	8.692386859892631E-5	0.0	0.0	13.866530138243721	0.0
12	2.607716057967789E-4	0.0	0.0	14.76280214736725	0.0
13	3.4769547439570525E-4	0.0	0.0	15.111540708186142	0.0
14	3.4769547439570525E-4	0.0	0.0	15.373007704931712	0.0
15	3.4769547439570525E-4	0.0	0.0	16.318304775945037	0.0
16	3.4769547439570525E-4	0.0	0.0	17.320710828627856	0.0
17	3.4769547439570525E-4	0.0	0.0	18.797199660649216	0.0
18	3.4769547439570525E-4	0.0	0.0	19.38854273872771	0.0
19	3.4769547439570525E-4	0.0	0.0	20.09723303941476	0.0
20	3.4769547439570525E-4	0.0	0.0	20.99880740452282	0.0
21	3.4769547439570525E-4	0.0	0.0	21.81658716030152	0.0
22	3.4769547439570525E-4	0.0	0.0	22.748758727156407	0.0
23	3.4769547439570525E-4	0.0	0.0	23.400600817779758	0.0
24	3.4769547439570525E-4	0.0	0.0	23.989249255931686	0.0
25	3.4769547439570525E-4	0.0	0.0	24.546952796862396	0.0
26	3.4769547439570525E-4	0.0	0.0	25.089183889182497	0.0
27	3.4769547439570525E-4	0.0	0.0	25.649668993908374	0.0
28	4.346193429946316E-4	0.0	0.0	26.177644571778252	0.0
29	5.215432115935579E-4	0.0	0.0	26.806538761091485	0.0
30	5.215432115935579E-4	0.0	0.0	27.44986231259214	0.0
31	5.215432115935579E-4	0.0	0.0	27.985226419292925	0.0
32	5.215432115935579E-4	0.0	0.0	28.523545937526077	0.0
33	5.215432115935579E-4	0.0	0.0	29.04865302773219	0.0
34	5.215432115935579E-4	0.0	0.0	29.814191538482934	0.0
35	5.215432115935579E-4	0.0	0.0	30.347817167811744	0.0
36	5.215432115935579E-4	0.0	0.0	30.902565297210092	0.0
37	5.215432115935579E-4	0.0	0.0	31.43688631748769	0.0
38	5.215432115935579E-4	0.0	0.0	32.05500194709466	0.0
39	6.084670801924842E-4	0.0	0.0	32.60253539539929	0.0
40	6.084670801924842E-4	0.0	0.0	33.14554880253679	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	35	1.01957994E-7	46.000004	39
CGACGGT	150	0.0	46.0	27
GCGAACG	20	6.311458E-4	46.0	24
TAGGTCG	20	6.311458E-4	46.0	41
ATTCGGC	30	1.8614046E-6	46.0	16
TACGGGT	120	0.0	46.0	3
TTATCGG	25	3.417158E-5	46.0	1
TACTCGC	25	3.417158E-5	46.0	44
CCGAATA	20	6.311458E-4	46.0	10
GCGTATA	20	6.311458E-4	46.0	30
ATAGCGG	185	0.0	44.756756	1
CACGACG	175	0.0	44.685715	25
CGGGATA	165	0.0	44.606064	5
CGGTCTA	155	0.0	44.51613	30
ACGGGAT	205	0.0	43.756096	4
CGGGTAT	285	0.0	43.57895	5
TACGCGG	185	0.0	43.513515	1
CTAGGGT	150	0.0	42.93333	3
CGTTTTT	1715	0.0	42.915455	1
CATGCGG	215	0.0	42.790695	1
>>END_MODULE