FastQCFastQC Report
Thu 26 May 2016
SRR1545007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545007_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences713377
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA71621.0039572343935956No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT39070.5476767543669055No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC32180.45109388163621755No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT26020.36474402735159667Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT25150.35254851221724276No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21390.2998414583032534No Hit
TGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTTG16440.23045318253882588Illumina Single End Adapter 2 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15860.2223228391159233No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12800.17942826864336808No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT10470.14676671661688No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10150.14228100990079579No Hit
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC9730.13639351983593528No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8520.11943194131574188No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA8420.11803015796696557No Hit
GCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC8080.1132640945811261No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC7580.10625517783724454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGG750.046.0000041
AATCCGG206.309837E-446.01
ATCTCGG301.8604042E-646.01
CCGATTC206.309837E-446.010
CCAATCG206.309837E-446.023
AACGACC206.309837E-446.023
GCGATAC253.415843E-546.08
CGTTTTT12000.044.0833361
CGAGGGT1100.043.9090923
CGGTCTA950.043.57894530
AGGGCGA3600.043.4444475
CACGACG850.043.29411725
ATAGCGG1600.043.1251
TACGGGT700.042.7142873
AGTACGG700.042.7142871
TACGCGG601.8189894E-1242.1666681
TCGAAGG601.8189894E-1242.1666681
GGGCGAT7100.042.1126756
CGGGATA1100.041.8181845
CGGGTAT1550.041.5483865
Produced by FastQC (version 0.11.5)