FastQCFastQC Report
Thu 26 May 2016
SRR1545006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545006_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1397529
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC77390.553763106168101No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG52880.3783821301740429No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT51620.36936621708744505Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT50350.36027874913508057Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT42400.30339263085059415Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.2641805644104702No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27930.19985274008625223No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT19520.13967509797649996No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16840.12049839395103787No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC15860.1134860171059062No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT15110.1081194021734075Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC14500.10375455536164187No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC14140.1011785801940425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT206.3119235E-446.00000421
AATACGA206.3119235E-446.00000417
AATGCGG1650.044.606061
CGTTTTT24350.043.166321
ATAGCGG1500.042.9333341
TAGTAGG2800.041.892861
CGCACTT10000.041.8634
GCATAGG2750.041.818181
CATGCGG2100.041.6190451
CACTTAC10050.041.42288636
ACGTAGG1000.041.41
ATACCGG508.731149E-1041.41
ATCAACG11450.041.37991714
TTATGAC10900.041.35779626
CAACGCA11250.041.29777516
AACGCAA11300.041.11504417
TATGACC10800.041.10185227
ACGCAAG11250.041.0933318
GCTTATG11000.040.98181524
TACGCGG1800.040.888891
Produced by FastQC (version 0.11.5)