##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545006_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1397529
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86484716953995	34.0	33.0	34.0	31.0	34.0
2	33.18445627961924	34.0	33.0	34.0	31.0	34.0
3	33.244171677296144	34.0	33.0	34.0	31.0	34.0
4	36.549192181342924	37.0	37.0	37.0	35.0	37.0
5	36.49605124473267	37.0	37.0	37.0	35.0	37.0
6	36.59964551719499	37.0	37.0	37.0	35.0	37.0
7	36.45271618692707	37.0	37.0	37.0	35.0	37.0
8	36.48256100588968	37.0	37.0	37.0	35.0	37.0
9	38.29306082378255	39.0	39.0	39.0	37.0	39.0
10	38.109168396505545	39.0	39.0	39.0	35.0	39.0
11	37.51396715202332	39.0	35.0	39.0	35.0	39.0
12	37.41920346554526	39.0	35.0	39.0	35.0	39.0
13	37.342344237579326	39.0	35.0	39.0	35.0	39.0
14	38.70915880815353	41.0	36.0	41.0	35.0	41.0
15	38.76840981475161	41.0	36.0	41.0	35.0	41.0
16	38.77557818120411	41.0	36.0	41.0	35.0	41.0
17	38.81729609904338	41.0	36.0	41.0	35.0	41.0
18	38.80120841857307	41.0	36.0	41.0	35.0	41.0
19	38.729366617794696	41.0	36.0	41.0	35.0	41.0
20	38.66248714695724	41.0	35.0	41.0	35.0	41.0
21	38.61821185821547	41.0	35.0	41.0	35.0	41.0
22	38.55926925308884	40.0	35.0	41.0	35.0	41.0
23	38.54994279188482	40.0	35.0	41.0	35.0	41.0
24	38.49823009039526	40.0	35.0	41.0	35.0	41.0
25	38.42318620937383	40.0	35.0	41.0	35.0	41.0
26	38.36280892918859	40.0	35.0	41.0	35.0	41.0
27	38.307187900930856	40.0	35.0	41.0	35.0	41.0
28	38.34524793403214	40.0	35.0	41.0	35.0	41.0
29	38.34657169904882	40.0	35.0	41.0	35.0	41.0
30	38.271673074404895	40.0	35.0	41.0	35.0	41.0
31	38.238930283378735	40.0	35.0	41.0	35.0	41.0
32	38.15223870130781	40.0	35.0	41.0	35.0	41.0
33	38.007378737757854	40.0	35.0	41.0	35.0	41.0
34	37.94643903632769	40.0	35.0	41.0	35.0	41.0
35	37.86546683467749	40.0	35.0	41.0	35.0	41.0
36	37.77885181631294	40.0	35.0	41.0	34.0	41.0
37	37.743855047015124	40.0	35.0	41.0	35.0	41.0
38	37.64557300778731	40.0	35.0	41.0	35.0	41.0
39	37.54024853867075	40.0	35.0	41.0	34.0	41.0
40	37.44734599425128	40.0	35.0	41.0	34.0	41.0
41	37.41365438570506	39.0	35.0	41.0	34.0	41.0
42	37.34510768649523	39.0	35.0	41.0	34.0	41.0
43	37.26916650745709	39.0	35.0	41.0	34.0	41.0
44	37.2061853457066	39.0	35.0	41.0	34.0	41.0
45	37.13555711545163	39.0	35.0	41.0	34.0	41.0
46	37.138514478053764	39.0	35.0	41.0	34.0	41.0
47	37.066080202986846	39.0	35.0	41.0	34.0	41.0
48	37.04322557886098	39.0	35.0	41.0	34.0	41.0
49	37.0122358820461	39.0	35.0	41.0	34.0	41.0
50	36.941880275829696	38.0	35.0	41.0	34.0	41.0
51	36.88176703309914	38.0	35.0	41.0	34.0	41.0
52	36.666934281864634	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	7.0
14	17.0
15	35.0
16	46.0
17	80.0
18	146.0
19	265.0
20	435.0
21	736.0
22	1170.0
23	1892.0
24	3091.0
25	4904.0
26	6789.0
27	7856.0
28	8356.0
29	9157.0
30	10666.0
31	13137.0
32	16912.0
33	24441.0
34	82518.0
35	226965.0
36	58754.0
37	92701.0
38	157607.0
39	664343.0
40	4494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.06842577148667	21.4764774112022	22.563395822197606	18.89170099511352
2	30.959285996927434	23.099484876521345	23.99506557645673	21.94616355009449
3	29.7095802663129	23.891883460021223	24.15205695194876	22.246479321717118
4	27.761284381218566	25.784008775488736	21.53329197462092	24.921414868671775
5	24.815299002739835	34.51033932032895	22.06072289018689	18.613638786744318
6	84.34594201623008	2.8281345145610577	9.77739996808653	3.0485235011223377
7	85.74441031277348	3.0716357227649658	6.604299445664455	4.579654518797105
8	74.71451397430751	5.211126209187788	12.99078587993523	7.083573936569473
9	50.89590269683134	18.330925512100286	16.05247547635863	14.72069631470975
10	40.19158099760362	20.25381941984746	25.146311811776357	14.408287770772557
11	36.878733822339285	18.30115868794136	24.74431657589932	20.075790913820036
12	24.125366987017802	25.31024400924775	30.72916554862189	19.835223455112562
13	18.94229028521054	27.273566416153084	32.90550679091454	20.878636507721843
14	17.954618473033477	22.284975839499573	39.57957223070147	20.180833456765477
15	20.619965667975404	19.606605658988112	38.75189709837864	21.021531574657843
16	22.36833725811772	21.358340327821463	28.049507380526627	28.223815033534187
17	21.997683053446476	22.61699041665683	30.573533715579426	24.811792814317272
18	24.077496781819914	26.472223474432372	28.49171645096452	20.958563292783193
19	25.49227958775811	23.614035916249325	25.48948894799321	25.404195547999365
20	23.255188264429574	29.019004256798965	26.79751189420756	20.928295584563898
21	22.399463624726213	22.702283816650674	28.044426985057196	26.853825573565913
22	20.909333545135738	25.840036235384023	26.659053228949098	26.591576990531145
23	22.098360749580152	22.24791041903245	32.733202674148444	22.920526157238953
24	24.55355130376543	20.904181594800537	29.330053258286593	25.212213843147442
25	18.971198450980268	25.814419593439563	29.42665232707157	25.787729628508604
26	19.65562074203827	25.668733886738664	28.060669939586226	26.61497543163684
27	22.434597063817638	24.524213808801104	30.71957719660916	22.321611930772097
28	20.076005578417337	24.72227767724319	27.887006280370567	27.314710463968904
29	22.308660500068335	23.70169062681347	27.13646729334418	26.853181579774017
30	26.253551804649494	22.628582304911024	25.513531382890804	25.604334507548682
31	28.543164399450742	23.025640255050163	22.634163584440824	25.79703176105827
32	25.78458121441487	22.505364826060855	23.918573424952182	27.791480534572095
33	19.662060679957268	24.039501148097823	28.982368165526438	27.31607000641847
34	22.69512833007401	21.460019792075872	27.67212701847332	28.172724859376803
35	28.0362697303598	22.71008329701924	23.518223950987778	25.73542302163318
36	23.219267721814717	25.118834743321962	26.216486384182367	25.445411150680954
37	24.23069574942631	25.365055036425005	26.3571632502796	24.047085963869087
38	23.918072540891817	23.234294243625715	26.240886593408796	26.606746622073672
39	27.52150402603452	21.12671722733482	27.028133226573477	24.323645520057184
40	20.717065620820748	20.109779475059195	31.338741450087976	27.83441345403208
41	21.849636036175276	22.95673291931688	25.835385169109195	29.358245875398648
42	21.678906126456052	19.906062772221542	29.508868867837446	28.906162233484956
43	22.7376319203394	23.909700621597118	27.661393788608322	25.69127366945516
44	20.398789577890692	24.790970348379172	26.234375100624028	28.575864973106103
45	22.62156992806589	23.885801296431058	26.073877536709432	27.418751238793615
46	24.822168269853435	20.910049093793404	28.114550753508517	26.15323188284465
47	22.352881407112125	20.30483803913908	30.84079113921786	26.501489414530933
48	21.953676810999987	20.017831472549048	27.957416268284952	30.071075448166013
49	22.53477387589095	21.94079693516199	29.890327857239456	25.63410133170761
50	21.606206382837136	22.610478923872062	28.775717713192357	27.007596980098448
51	21.593040287536073	19.91915731265684	28.53114318200195	29.956659217805143
52	22.456564407608	18.249496074857838	32.683972926500985	26.609966591033174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	238.0
1	543.5
2	849.0
3	3827.0
4	6805.0
5	4403.5
6	2002.0
7	2182.0
8	2362.0
9	2265.5
10	2169.0
11	2150.5
12	2132.0
13	1983.5
14	1770.0
15	1705.0
16	1749.5
17	1794.0
18	1736.0
19	1678.0
20	1918.5
21	2159.0
22	2374.5
23	2590.0
24	2808.0
25	3026.0
26	3965.0
27	4904.0
28	6345.5
29	7787.0
30	8864.0
31	9941.0
32	11157.5
33	12374.0
34	15025.0
35	17676.0
36	20591.0
37	23506.0
38	25173.0
39	29410.5
40	31981.0
41	40837.5
42	49694.0
43	62146.0
44	74598.0
45	81517.0
46	88436.0
47	94392.5
48	100349.0
49	119879.5
50	139410.0
51	147330.5
52	155251.0
53	145285.5
54	135320.0
55	117934.5
56	100549.0
57	90341.5
58	80134.0
59	76318.5
60	72503.0
61	66655.0
62	60807.0
63	56713.5
64	45045.5
65	37471.0
66	32202.5
67	26934.0
68	22395.5
69	17857.0
70	15704.0
71	13551.0
72	11551.5
73	9552.0
74	8012.5
75	6473.0
76	5725.0
77	4977.0
78	3788.5
79	2600.0
80	1756.5
81	913.0
82	850.0
83	787.0
84	490.0
85	193.0
86	153.5
87	114.0
88	70.5
89	38.0
90	49.0
91	27.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1397529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.668952603463346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.31925644359714	18.050216607614793
2	11.844705249814751	6.0808235531897665
3	4.314259043585186	3.322275327265537
4	2.0962801237759776	2.152372605631514
5	1.231422462583682	1.5804662413450328
6	0.8070573878318734	1.2429790701918775
7	0.6270933742598819	1.1267781071275966
8	0.5141623122446635	1.055840641879631
9	0.44513092537336985	1.028344016317033
>10	7.1713605904834585	45.16470018548945
>50	0.4921123146121443	8.200267900265837
>100	0.12092453355780557	5.419364757162945
>500	0.01035696235113412	1.782360549132627
>1k	0.004758604323494055	2.1245197418670285
>5k	0.0011196716055280132	1.6686906955194765
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	7739	0.553763106168101	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5288	0.3783821301740429	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	5162	0.36936621708744505	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	5035	0.36027874913508057	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	4240	0.30339263085059415	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3692	0.2641805644104702	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2793	0.19985274008625223	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1952	0.13967509797649996	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1684	0.12049839395103787	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1586	0.1134860171059062	No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	1511	0.1081194021734075	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1450	0.10375455536164187	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1414	0.1011785801940425	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.155486576664956E-5	0.0	0.0	0.3104765625614925	0.0
2	7.155486576664956E-5	0.0	0.0	1.2527825898425007	0.0
3	7.155486576664956E-5	0.0	0.0	1.9836439887830593	0.0
4	7.155486576664956E-5	0.0	0.0	2.4584820780105456	0.0
5	7.155486576664956E-5	0.0	0.0	5.205902703986823	0.0
6	7.155486576664956E-5	0.0	0.0	5.740560660995228	0.0
7	7.155486576664956E-5	0.0	0.0	7.632256647268142	0.0
8	7.155486576664956E-5	0.0	0.0	9.786129661710062	0.0
9	7.155486576664956E-5	0.0	0.0	11.583301670305232	0.0
10	7.155486576664956E-5	0.0	0.0	12.698555808144231	0.0
11	7.155486576664956E-5	0.0	0.0	13.791914157058637	0.0
12	7.155486576664956E-5	0.0	0.0	14.706456896422186	0.0
13	7.155486576664956E-5	0.0	0.0	15.16125962323501	0.0
14	7.155486576664956E-5	0.0	0.0	15.423794425732847	0.0
15	7.155486576664956E-5	0.0	0.0	16.31114631610507	0.0
16	1.4310973153329912E-4	0.0	0.0	17.214454941543252	0.0
17	1.4310973153329912E-4	0.0	0.0	18.376291296996342	0.0
18	1.4310973153329912E-4	0.0	0.0	19.057493619094846	0.0
19	1.4310973153329912E-4	0.0	0.0	19.752720694883614	0.0
20	2.146645972999487E-4	0.0	0.0	20.61409816898254	0.0
21	2.146645972999487E-4	0.0	0.0	21.39404620583902	0.0
22	2.146645972999487E-4	0.0	0.0	22.20769658447159	0.0
23	2.146645972999487E-4	0.0	0.0	22.835805196171243	0.0
24	4.293291945998974E-4	0.0	0.0	23.37997995032661	0.0
25	4.293291945998974E-4	0.0	0.0	23.93953900062181	0.0
26	4.293291945998974E-4	0.0	0.0	24.474697841690585	0.0
27	4.293291945998974E-4	0.0	0.0	24.964777117326367	0.0
28	5.00884060366547E-4	0.0	0.0	25.500508397321273	0.0
29	5.724389261331965E-4	0.0	0.0	26.11137228637116	0.0
30	5.724389261331965E-4	0.0	0.0	27.020119081607607	0.0
31	5.724389261331965E-4	0.0	0.0	27.56014365354851	0.0
32	5.724389261331965E-4	0.0	0.0	28.11483697297158	0.0
33	5.724389261331965E-4	0.0	0.0	28.745163785509998	0.0
34	5.724389261331965E-4	0.0	0.0	29.480890915322686	0.0
35	5.724389261331965E-4	0.0	0.0	30.043669934577387	0.0
36	6.439937918998461E-4	0.0	0.0	30.601153893765353	0.0
37	7.155486576664957E-4	0.0	0.0	31.189692664696047	0.0
38	7.155486576664957E-4	0.0	0.0	31.94795957722523	0.0
39	7.155486576664957E-4	0.0	0.0	32.50852039564116	0.0
40	7.155486576664957E-4	0.0	0.0	33.04511033402527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	20	6.3119235E-4	46.000004	21
AATACGA	20	6.3119235E-4	46.000004	17
AATGCGG	165	0.0	44.60606	1
CGTTTTT	2435	0.0	43.16632	1
ATAGCGG	150	0.0	42.933334	1
TAGTAGG	280	0.0	41.89286	1
CGCACTT	1000	0.0	41.86	34
GCATAGG	275	0.0	41.81818	1
CATGCGG	210	0.0	41.619045	1
CACTTAC	1005	0.0	41.422886	36
ACGTAGG	100	0.0	41.4	1
ATACCGG	50	8.731149E-10	41.4	1
ATCAACG	1145	0.0	41.379917	14
TTATGAC	1090	0.0	41.357796	26
CAACGCA	1125	0.0	41.297775	16
AACGCAA	1130	0.0	41.115044	17
TATGACC	1080	0.0	41.101852	27
ACGCAAG	1125	0.0	41.09333	18
GCTTATG	1100	0.0	40.981815	24
TACGCGG	180	0.0	40.88889	1
>>END_MODULE