FastQCFastQC Report
Thu 26 May 2016
SRR1545005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545005_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences808962
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC40750.5037319429095557No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT38180.47196283632605734Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG31050.38382519821697436No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT30760.3802403573962683Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT30570.37789166858270223Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA30380.37554297976913625No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.29519309930503534No Hit
TGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTG19370.23944264378302071Illumina Single End Adapter 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15960.19728986033954624No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT14120.17454466340817No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC10500.12979596074970148No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9680.11965951429115335No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC9560.11817613188258534No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT8630.10667991821618321Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC8560.10581461181118519No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCG8100.10012831257834114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG206.310339E-446.00000431
CGTACCC206.310339E-446.00000435
TCGCCTA253.4162527E-546.024
ATTACGG453.092282E-1046.01
CGGTCTA600.046.030
ATATTGG551.8189894E-1246.01
CGAATAT351.0190706E-745.99999614
CGTTTTT15900.044.1194951
AATGCGG800.043.1250041
TACGGGA1500.042.933333
CTCACGA700.042.71428323
ATAGCGG1250.042.321
GGCGATA1250.042.327
ACGGGAT1800.042.1666684
GGGCGAT6650.041.849626
GCGATAG554.7293724E-1141.818188
ACACGAC2000.041.39999825
CTAGCGG1350.040.888891
TAGTAGG1300.040.692311
CGACGGT850.040.58823427
Produced by FastQC (version 0.11.5)