##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545005_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	808962
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.852513467876115	34.0	33.0	34.0	31.0	34.0
2	33.236566118062406	34.0	33.0	34.0	31.0	34.0
3	33.36316662587365	34.0	34.0	34.0	31.0	34.0
4	36.58911172589071	37.0	37.0	37.0	35.0	37.0
5	36.55839458466529	37.0	37.0	37.0	35.0	37.0
6	36.35047258091233	37.0	37.0	37.0	35.0	37.0
7	36.186965518775914	37.0	37.0	37.0	35.0	37.0
8	36.340203866189015	37.0	37.0	37.0	35.0	37.0
9	38.24607336314932	39.0	39.0	39.0	37.0	39.0
10	38.140819964349376	39.0	39.0	39.0	35.0	39.0
11	37.59568187380866	39.0	37.0	39.0	35.0	39.0
12	37.55423616931327	39.0	37.0	39.0	35.0	39.0
13	37.48858660851808	39.0	37.0	39.0	35.0	39.0
14	38.771131400486055	41.0	37.0	41.0	35.0	41.0
15	38.79978787631558	41.0	37.0	41.0	35.0	41.0
16	38.82680645073563	41.0	37.0	41.0	35.0	41.0
17	38.90228836459562	41.0	37.0	41.0	35.0	41.0
18	38.89826098135636	41.0	37.0	41.0	35.0	41.0
19	38.85828629774946	41.0	37.0	41.0	35.0	41.0
20	38.812347180708116	41.0	35.0	41.0	35.0	41.0
21	38.75865491827799	41.0	35.0	41.0	35.0	41.0
22	38.70951911214618	41.0	35.0	41.0	35.0	41.0
23	38.680035650623886	41.0	35.0	41.0	35.0	41.0
24	38.64302155107409	41.0	35.0	41.0	35.0	41.0
25	38.56045895851721	40.0	35.0	41.0	35.0	41.0
26	38.48559759296481	40.0	35.0	41.0	35.0	41.0
27	38.55850831064994	40.0	36.0	41.0	35.0	41.0
28	38.559986748450484	40.0	36.0	41.0	35.0	41.0
29	38.575929400886565	40.0	36.0	41.0	35.0	41.0
30	38.519758653682125	40.0	35.0	41.0	35.0	41.0
31	38.46737053161953	40.0	35.0	41.0	35.0	41.0
32	38.38228248051206	40.0	35.0	41.0	35.0	41.0
33	38.254152852667985	40.0	35.0	41.0	35.0	41.0
34	38.16479018791983	40.0	35.0	41.0	35.0	41.0
35	38.098338364472	40.0	35.0	41.0	35.0	41.0
36	37.987521045487924	40.0	35.0	41.0	35.0	41.0
37	37.974171839962814	40.0	35.0	41.0	35.0	41.0
38	37.875859434682965	40.0	35.0	41.0	35.0	41.0
39	37.79379995599299	40.0	35.0	41.0	35.0	41.0
40	37.71683342357243	40.0	35.0	41.0	35.0	41.0
41	37.69272351482517	40.0	35.0	41.0	35.0	41.0
42	37.64437019291388	40.0	35.0	41.0	35.0	41.0
43	37.6015066220663	40.0	35.0	41.0	35.0	41.0
44	37.48137860616444	39.0	35.0	41.0	35.0	41.0
45	37.485811447262044	39.0	35.0	41.0	35.0	41.0
46	37.41897023593197	39.0	35.0	41.0	35.0	41.0
47	37.3219360118275	39.0	35.0	41.0	35.0	41.0
48	37.23936723851059	39.0	35.0	41.0	34.0	41.0
49	37.22090036367592	39.0	35.0	41.0	34.0	41.0
50	37.157742885327124	39.0	35.0	41.0	34.0	41.0
51	37.08669875717277	39.0	35.0	41.0	34.0	41.0
52	36.62457074621552	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	7.0
14	5.0
15	15.0
16	26.0
17	38.0
18	87.0
19	110.0
20	231.0
21	346.0
22	585.0
23	912.0
24	1537.0
25	2369.0
26	3535.0
27	3993.0
28	4001.0
29	4090.0
30	4737.0
31	6002.0
32	7751.0
33	11957.0
34	46685.0
35	128316.0
36	32490.0
37	53647.0
38	95220.0
39	398742.0
40	1526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.18241647938964	21.133996405269965	21.88767828402323	20.79590883131717
2	30.82382114363839	23.23149913098514	24.01707373152262	21.927605993853852
3	29.88310946620484	23.642272442957765	24.240693629614245	22.233924461223147
4	27.700065021595577	25.30452604695894	22.108949493301292	24.88645943814419
5	24.71092585313031	33.79528333840155	22.380037628467097	19.11375318000104
6	83.15384406189659	2.8614446661277047	10.332376551679808	3.652334720295886
7	84.59321945901044	3.003849377350234	7.287116082090382	5.1158150815489485
8	72.99428156081497	5.263658861602893	13.818077981413218	7.923981596168918
9	50.77061716125109	17.70763027187927	16.491133081652784	15.030619485216858
10	39.485785488069894	19.83084495934296	26.0731653649986	14.610204187588538
11	35.999836827935056	18.042874696215645	24.96062855857259	20.99665991727671
12	23.722869553823294	24.488789337447248	31.18317053211399	20.605170576615464
13	18.93030822214146	26.425345071832794	33.043455687659	21.600891018366745
14	17.70750665667856	22.011417099937944	39.03384831425951	21.247227929123987
15	20.464002017400077	19.24169491274003	38.663991633723214	21.63031143613668
16	22.079034614728503	21.695703877314383	28.267582408073554	27.957679099883553
17	21.734766280740008	22.712686133588473	30.647669482620937	24.904878103050578
18	23.60222111792643	26.473678615311968	28.6219871885206	21.302113078241007
19	25.128374385941495	23.52718669109303	25.67302296028738	25.6714159626781
20	22.94730778454365	29.105199008111633	26.87505717203033	21.07243603531439
21	22.070999626682095	22.538759546183876	28.041984666770503	27.348256160363526
22	20.731628926945888	25.73668478865509	26.866651338381782	26.66503494601724
23	21.78359428502204	22.178792081704703	32.60659956833572	23.431014064937536
24	24.29570239393198	21.240923553887576	28.766864203757407	25.696509848423045
25	19.081242382213258	25.544710381946246	29.492485431948595	25.881561803891902
26	19.701543459396113	24.989801745941094	27.9704114655571	27.338243329105694
27	22.85434915360672	23.83511215607161	30.477945812040613	22.83259287828106
28	20.39242881618667	24.422902435466685	27.65408016692997	27.53058858141668
29	22.7084832167642	23.096634947006166	26.70644603825643	27.488435797973203
30	26.080335046640013	22.570281422365944	25.191912599108484	26.157470931885552
31	28.312083880330597	22.523184030893912	22.260872574978798	26.90385951379669
32	25.36472664970666	22.167048637636874	24.12400088014023	28.344223832516235
33	19.452335214756687	23.853283590576567	28.91223567979707	27.782145514869672
34	23.178839055480974	20.823722251477818	27.63071689399502	28.366721799046186
35	27.749140256279038	22.30685742964441	23.847968136945862	26.096034177130694
36	23.541526054375854	24.65134332638616	25.83026149559559	25.9768691236424
37	24.75369671257735	24.970517774629712	26.332262825695153	23.943522687097786
38	24.220915197500005	22.75483891703195	25.898002625586862	27.126243259881182
39	28.01429486181057	20.60714841982689	26.954541746089433	24.424014972273113
40	20.81667618503712	19.416734036951055	31.703961372722077	28.06262840528974
41	22.047018277743575	22.761884983472648	25.888731485533313	29.30236525325046
42	21.646010566627357	19.38817892558612	29.557754257925588	29.40805624986093
43	23.148182485703902	23.66303979667772	27.446777475332585	25.742000242285794
44	20.761420190317963	23.868982721067244	26.823386018131878	28.54621107048291
45	22.625290186683674	23.205169093233057	26.25042956282248	27.919111157260783
46	24.958279869759025	20.037653190137487	28.263008645647137	26.741058294456355
47	22.18151161612041	19.831586650547244	31.0931786659942	26.893723067338144
48	21.79657388109701	19.45480751877097	27.84543649763524	30.90318210249678
49	22.60402837216087	21.020146805412367	30.13862208608068	26.23720273634608
50	21.701513791747946	22.039601365700737	28.962917912089814	27.295966930461507
51	21.754421097653537	19.132913536111708	28.751906764471013	30.36075860176374
52	22.444441148039093	18.146340619213262	32.89289237318935	26.5163258595583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	356.0
2	597.0
3	2470.0
4	4343.0
5	2854.5
6	1366.0
7	1362.5
8	1359.0
9	1385.0
10	1411.0
11	1349.0
12	1287.0
13	1210.0
14	1082.5
15	1032.0
16	1046.5
17	1061.0
18	1052.5
19	1044.0
20	1124.0
21	1204.0
22	1376.5
23	1549.0
24	1579.5
25	1610.0
26	2109.5
27	2609.0
28	3184.5
29	3760.0
30	4375.5
31	4991.0
32	5645.0
33	6299.0
34	7863.0
35	9427.0
36	10860.5
37	12294.0
38	13365.0
39	15609.5
40	16783.0
41	21503.5
42	26224.0
43	32782.0
44	39340.0
45	43710.0
46	48080.0
47	52201.5
48	56323.0
49	70158.5
50	83994.0
51	88859.0
52	93724.0
53	87903.0
54	82082.0
55	71080.0
56	60078.0
57	53724.5
58	47371.0
59	44632.5
60	41894.0
61	38605.5
62	35317.0
63	34242.5
64	27692.0
65	22216.0
66	18920.0
67	15624.0
68	13105.0
69	10586.0
70	9212.5
71	7839.0
72	6764.0
73	5689.0
74	4868.0
75	4047.0
76	3496.0
77	2945.0
78	2226.5
79	1508.0
80	1013.5
81	519.0
82	493.5
83	468.0
84	300.5
85	133.0
86	93.0
87	53.0
88	30.0
89	12.5
90	18.0
91	11.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	808962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.115515327289327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7830001378992	22.335650410296143
2	10.367432646900482	6.4517601885854345
3	3.627105083996561	3.3857773150435215
4	1.9079098592782198	2.374623938778315
5	1.2444144215915756	1.936029800426628
6	0.9916389021580369	1.8513213275540854
7	0.8401428602441015	1.8299034631526625
8	0.7277143025449002	1.8114564427778805
9	0.6624588524234234	1.8551473718651574
>10	7.576328629599619	42.805769388164016
>50	0.18854411445671687	3.9080800994495313
>100	0.07135178858551916	4.285505158709928
>500	0.007573653537010409	1.627653513479694
>1k	0.004384746784584974	3.5413215817170016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	4075	0.5037319429095557	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	3818	0.47196283632605734	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3105	0.38382519821697436	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	3076	0.3802403573962683	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	3057	0.37789166858270223	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3038	0.37554297976913625	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2388	0.29519309930503534	No Hit
TGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTG	1937	0.23944264378302071	Illumina Single End Adapter 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1596	0.19728986033954624	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1412	0.17454466340817	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1050	0.12979596074970148	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	968	0.11965951429115335	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	956	0.11817613188258534	No Hit
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	863	0.10667991821618321	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	856	0.10581461181118519	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCG	810	0.10012831257834114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.236152007140014E-4	0.0	0.0	0.3860502718298264	0.0
2	1.236152007140014E-4	0.0	0.0	1.4533439147945144	0.0
3	1.236152007140014E-4	0.0	0.0	2.2420088953498434	0.0
4	1.236152007140014E-4	0.0	0.0	2.7888825433085858	0.0
5	1.236152007140014E-4	0.0	0.0	5.717573878624707	0.0
6	1.236152007140014E-4	0.0	0.0	6.2923845619448135	0.0
7	1.236152007140014E-4	0.0	0.0	8.40509195734781	0.0
8	1.236152007140014E-4	0.0	0.0	10.761197682956677	0.0
9	1.236152007140014E-4	0.0	0.0	12.773158689777764	0.0
10	1.236152007140014E-4	0.0	0.0	14.04577718112841	0.0
11	1.236152007140014E-4	0.0	0.0	15.214806134280721	0.0
12	1.236152007140014E-4	0.0	0.0	16.205458352802726	0.0
13	1.236152007140014E-4	0.0	0.0	16.699548310056592	0.0
14	1.236152007140014E-4	0.0	0.0	16.991651029343775	0.0
15	1.236152007140014E-4	0.0	0.0	17.957332977321556	0.0
16	1.236152007140014E-4	0.0	0.0	18.90187672597724	0.0
17	2.472304014280028E-4	0.0	0.0	20.062747075882427	0.0
18	2.472304014280028E-4	0.0	0.0	20.77464701679436	0.0
19	3.708456021420042E-4	0.0	0.0	21.490255413727716	0.0
20	3.708456021420042E-4	0.0	0.0	22.387207310108508	0.0
21	3.708456021420042E-4	0.0	0.0	23.183907278710247	0.0
22	3.708456021420042E-4	0.0	0.0	24.03660493323543	0.0
23	3.708456021420042E-4	0.0	0.0	24.668278608883977	0.0
24	7.416912042840084E-4	0.0	0.0	25.236414071365527	0.0
25	7.416912042840084E-4	0.0	0.0	25.795896469797096	0.0
26	7.416912042840084E-4	0.0	0.0	26.33597128171657	0.0
27	7.416912042840084E-4	0.0	0.0	26.824869400540447	0.0
28	8.653064049980098E-4	0.0	0.0	27.37619319572489	0.0
29	8.653064049980098E-4	0.0	0.0	27.96274732311283	0.0
30	8.653064049980098E-4	0.0	0.0	28.88578202684428	0.0
31	8.653064049980098E-4	0.0	0.0	29.419552463527335	0.0
32	8.653064049980098E-4	0.0	0.0	29.965684420281793	0.0
33	8.653064049980098E-4	0.0	0.0	30.590435644690356	0.0
34	8.653064049980098E-4	0.0	0.0	31.304931504817283	0.0
35	8.653064049980098E-4	0.0	0.0	31.852052383177455	0.0
36	8.653064049980098E-4	0.0	0.0	32.40560125197475	0.0
37	8.653064049980098E-4	0.0	0.0	32.98263700890771	0.0
38	8.653064049980098E-4	0.0	0.0	33.72099060277244	0.0
39	8.653064049980098E-4	0.0	0.0	34.28010215560187	0.0
40	8.653064049980098E-4	0.0	0.0	34.82166034992991	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	20	6.310339E-4	46.000004	31
CGTACCC	20	6.310339E-4	46.000004	35
TCGCCTA	25	3.4162527E-5	46.0	24
ATTACGG	45	3.092282E-10	46.0	1
CGGTCTA	60	0.0	46.0	30
ATATTGG	55	1.8189894E-12	46.0	1
CGAATAT	35	1.0190706E-7	45.999996	14
CGTTTTT	1590	0.0	44.119495	1
AATGCGG	80	0.0	43.125004	1
TACGGGA	150	0.0	42.93333	3
CTCACGA	70	0.0	42.714283	23
ATAGCGG	125	0.0	42.32	1
GGCGATA	125	0.0	42.32	7
ACGGGAT	180	0.0	42.166668	4
GGGCGAT	665	0.0	41.84962	6
GCGATAG	55	4.7293724E-11	41.81818	8
ACACGAC	200	0.0	41.399998	25
CTAGCGG	135	0.0	40.88889	1
TAGTAGG	130	0.0	40.69231	1
CGACGGT	85	0.0	40.588234	27
>>END_MODULE