FastQCFastQC Report
Thu 26 May 2016
SRR1545004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545004_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1449825
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT70030.48302381321883675Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT52600.3628024071870743Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT51420.35466349386994983Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.19940337626954976No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC23330.16091597261738486Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT22870.1577431759005397Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC17370.11980756298173917Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC17050.11760040004828169Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC16210.1118065973479558Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT15720.10842687910609901Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG14620.1008397565223389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAA351.0198164E-746.0000049
AGCGTTG206.312003E-446.030
AGCGTAA206.312003E-446.014
AATCCGG850.046.01
ACACCGT206.312003E-446.013
CCGCTAA301.8617429E-646.015
CGCTAAA301.8617429E-646.016
AGTTCGG301.8617429E-646.01
TTAACGA253.417601E-546.025
GCGATAC750.046.08
ACCGTCC206.312003E-446.011
CGGTCGG206.312003E-446.01
GATTCCG253.417601E-546.08
CGATATC206.312003E-446.09
CGATACG206.312003E-446.09
AGTACGG1050.046.01
TAGTCGG301.8617429E-646.01
GCGTAAC253.417601E-546.015
TCGCGGA206.312003E-446.02
TTAGCGG2900.045.2068941
Produced by FastQC (version 0.11.5)