##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545004_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1449825
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95981308088907	34.0	33.0	34.0	31.0	34.0
2	33.24317003776318	34.0	33.0	34.0	31.0	34.0
3	33.28630007069819	34.0	34.0	34.0	31.0	34.0
4	36.57914575897091	37.0	37.0	37.0	35.0	37.0
5	36.566374562447194	37.0	37.0	37.0	35.0	37.0
6	36.68485368923836	37.0	37.0	37.0	35.0	37.0
7	36.562838273584745	37.0	37.0	37.0	35.0	37.0
8	36.57566602865863	37.0	37.0	37.0	35.0	37.0
9	38.32013449899126	39.0	39.0	39.0	37.0	39.0
10	38.15477868018554	39.0	39.0	39.0	35.0	39.0
11	37.59196454744538	39.0	35.0	39.0	35.0	39.0
12	37.525812425637575	39.0	35.0	39.0	35.0	39.0
13	37.501304640215196	39.0	35.0	39.0	35.0	39.0
14	38.855477730070874	41.0	36.0	41.0	35.0	41.0
15	38.91968547928198	41.0	36.0	41.0	35.0	41.0
16	38.93440104840239	41.0	36.0	41.0	35.0	41.0
17	38.98650043970824	41.0	36.0	41.0	35.0	41.0
18	38.979274740054834	41.0	36.0	41.0	35.0	41.0
19	38.90702257168969	41.0	36.0	41.0	35.0	41.0
20	38.82454296208163	41.0	35.0	41.0	35.0	41.0
21	38.7860610763368	41.0	35.0	41.0	35.0	41.0
22	38.75741072198369	41.0	35.0	41.0	35.0	41.0
23	38.74762057489697	41.0	35.0	41.0	35.0	41.0
24	38.702382701360506	41.0	35.0	41.0	35.0	41.0
25	38.67984342937941	41.0	35.0	41.0	35.0	41.0
26	38.651788319279916	41.0	35.0	41.0	35.0	41.0
27	38.62144672632904	41.0	36.0	41.0	35.0	41.0
28	38.63870260203818	41.0	36.0	41.0	35.0	41.0
29	38.655606711154796	41.0	36.0	41.0	35.0	41.0
30	38.58837238977118	41.0	36.0	41.0	35.0	41.0
31	38.52430396772024	41.0	35.0	41.0	35.0	41.0
32	38.46447881640888	41.0	35.0	41.0	35.0	41.0
33	38.39214801786422	40.0	35.0	41.0	35.0	41.0
34	38.308756574069285	40.0	35.0	41.0	35.0	41.0
35	38.27062300622489	40.0	35.0	41.0	35.0	41.0
36	38.20904936802717	40.0	35.0	41.0	35.0	41.0
37	38.16231407238805	40.0	35.0	41.0	35.0	41.0
38	38.11622092321487	40.0	35.0	41.0	35.0	41.0
39	38.043668028900036	40.0	35.0	41.0	35.0	41.0
40	37.98088183056576	40.0	35.0	41.0	35.0	41.0
41	37.970703360750434	40.0	35.0	41.0	35.0	41.0
42	37.87295294259652	40.0	35.0	41.0	35.0	41.0
43	37.79773214008587	40.0	35.0	41.0	35.0	41.0
44	37.7519735140448	40.0	35.0	41.0	35.0	41.0
45	37.71145827944752	40.0	35.0	41.0	35.0	41.0
46	37.74149362854138	40.0	35.0	41.0	35.0	41.0
47	37.70108116496819	40.0	35.0	41.0	35.0	41.0
48	37.72619523045885	40.0	35.0	41.0	35.0	41.0
49	37.688096839273705	40.0	35.0	41.0	35.0	41.0
50	37.60490266066594	40.0	35.0	41.0	35.0	41.0
51	37.55402686531133	40.0	35.0	41.0	34.0	41.0
52	37.34929112134223	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	8.0
14	15.0
15	17.0
16	18.0
17	46.0
18	100.0
19	173.0
20	275.0
21	540.0
22	855.0
23	1529.0
24	2712.0
25	4554.0
26	6430.0
27	7258.0
28	7239.0
29	7917.0
30	8969.0
31	11178.0
32	14626.0
33	20725.0
34	57009.0
35	255666.0
36	42123.0
37	62398.0
38	121227.0
39	809100.0
40	7113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.351714862138536	23.0862345455486	25.24456399910334	20.317486593209523
2	24.99474074457262	24.268308244098424	27.98461883330747	22.752332178021486
3	25.3427827496422	25.214008587243285	26.58162191988688	22.86158674322763
4	25.498525684134293	27.982342696532342	23.1970065352715	23.32212508406187
5	23.44317417619368	35.284741261876434	20.975221147379855	20.29686341455003
6	87.8081837463142	2.0866656320590415	7.441173934785232	2.6639766868415156
7	88.89879813080888	1.6689600469022121	5.085717241736072	4.346524580552826
8	78.91711068577241	4.002621005983481	10.812891211008226	6.267377097235874
9	41.49666339040919	24.306105909333887	15.197765247529874	18.999465452727055
10	34.56486127636094	18.76309209732209	32.13574052040764	14.536306105909333
11	34.06445605504113	19.327298122187162	25.983618712603246	20.62462711016847
12	19.51428620695601	32.927284327418825	30.10190885106823	17.45652061455693
13	14.883658372562206	35.593813046402154	29.75952270101564	19.763005880020003
14	13.128618971255152	26.023485593088818	42.49450795785698	18.353387477799043
15	15.282672046626317	20.739123687341575	44.561516045039916	19.41668822099219
16	15.28805200627662	23.498353249530116	29.205455830876144	32.008138913317126
17	17.678340489369408	23.77645577914576	34.96387495042505	23.58132878105978
18	22.027624023589055	27.594433810977186	30.002172676012623	20.375769489421135
19	23.705550669908437	24.5238563274878	27.955235976755816	23.81535702584795
20	17.74552101115652	31.860190022933804	29.27456762023003	21.11972134567965
21	19.110513337816634	25.29491490352284	28.478816408876934	27.115755349783594
22	16.42287862328212	29.736830307106032	26.174262410980635	27.666028658631213
23	15.924611591054092	23.851637266566655	39.07140516958943	21.152345972789817
24	17.388995223561466	24.13125722069905	35.27467108099253	23.205076474746953
25	14.724190850619904	32.814856965495835	30.15743279361302	22.30351939027124
26	16.863552497715244	30.275171141344646	28.31672788095115	24.544548479988965
27	17.969892918110805	27.952477023088996	34.74391736933768	19.33371268946252
28	14.614522442363734	24.991016157122413	32.143810459883085	28.250650940630766
29	16.680151052713256	31.334333454037555	28.793337126894624	23.19217836635456
30	22.50085355129067	26.900832859138173	25.185522390633352	25.4127911989378
31	22.52699463728381	28.250375045264082	25.33664407773352	23.885986239718587
32	20.885555153208145	31.880537306226614	24.497646267652993	22.736261272912248
33	15.59491662786888	27.654992843963928	28.706637007914747	28.04345352025244
34	15.480075181487422	29.34409325263394	28.424465021640543	26.7513665442381
35	24.77023088993499	25.457072405290294	26.814201713999964	22.95849499077475
36	17.83008294104461	32.2957598330833	28.462814477609367	21.411342748262722
37	18.52202852068353	29.80649388719328	29.334643836325075	22.336833755798114
38	18.283585950028453	30.803579742382702	27.06623213146414	23.846602176124705
39	23.758453606469747	27.944958874346902	26.66625282361664	21.630334695566706
40	16.2323038987464	24.837273464038763	31.48648974876278	27.443932888452053
41	20.219198868828997	27.289638404635042	24.827755073888227	27.66340765264773
42	19.705343748383424	24.904867828875897	28.268101322573415	27.121687100167264
43	20.597865259600294	28.576069525632402	27.481454658320832	23.34461055644647
44	15.655613608538962	32.07807838877106	27.048160984946456	25.21814701774352
45	20.38977117928026	28.26492852585657	27.315986412153194	24.02931388270998
46	21.92457710413326	26.244960598692945	27.507871639680655	24.322590657493144
47	19.956684427430897	23.17369337678685	31.923783904954046	24.945838290828203
48	18.66197644543307	22.911592778438777	29.217526253168487	29.208904522959667
49	19.848947286741502	25.575707412963634	31.23163140379011	23.34371389650475
50	18.38194264825065	26.801372579449247	28.95266670115359	25.864018071146518
51	18.66839101270843	23.67671960409015	30.284413636128498	27.37047574707292
52	19.71858672598417	22.079285431000294	34.51340679047471	23.688721052540824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	187.0
1	366.5
2	546.0
3	2840.0
4	5134.0
5	3487.5
6	1841.0
7	2095.0
8	2349.0
9	2351.0
10	2353.0
11	2326.5
12	2300.0
13	2307.0
14	2265.0
15	2216.0
16	2104.5
17	1993.0
18	2053.5
19	2114.0
20	2110.5
21	2107.0
22	2889.5
23	3672.0
24	4108.5
25	4545.0
26	6445.0
27	8345.0
28	9828.5
29	11312.0
30	13901.5
31	16491.0
32	18091.5
33	19692.0
34	23617.5
35	27543.0
36	32386.5
37	37230.0
38	40844.0
39	52211.5
40	59965.0
41	79075.5
42	98186.0
43	132021.5
44	165857.0
45	190491.0
46	215125.0
47	211562.5
48	208000.0
49	178777.0
50	149554.0
51	127865.0
52	106176.0
53	90292.0
54	74408.0
55	62259.5
56	50111.0
57	44640.0
58	39169.0
59	33974.5
60	28780.0
61	25615.0
62	22450.0
63	18379.0
64	11054.0
65	7800.0
66	5819.5
67	3839.0
68	3126.5
69	2414.0
70	1872.5
71	1331.0
72	1306.5
73	1282.0
74	1199.5
75	1117.0
76	951.0
77	785.0
78	479.0
79	173.0
80	167.0
81	161.0
82	111.5
83	62.0
84	45.0
85	28.0
86	14.5
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1449825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.379816011126312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.28502515322947	15.497316923753846
2	13.7879345752736	6.447187470866876
3	5.8346659232546845	4.092402473162489
4	3.022646316474014	2.826756589834845
5	1.763375960084343	2.0613702752607574
6	1.1344741907769496	1.5914278709841894
7	0.7841213455994309	1.2832828953358038
8	0.5891655237137557	1.101966523562119
9	0.3873304355629884	0.8150142887074876
>10	4.570975823634772	26.991449691794934
>50	1.5889907452158303	24.486771949460984
>100	0.23918701036481393	8.986306152499445
>500	0.008563485552360132	1.4594816653511795
>1k	0.0026576334472841793	1.1576437353173405
>5k	8.858778157613931E-4	1.2016214941077237
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	7003	0.48302381321883675	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	5260	0.3628024071870743	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	5142	0.35466349386994983	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2891	0.19940337626954976	No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	2333	0.16091597261738486	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	2287	0.1577431759005397	Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1737	0.11980756298173917	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1705	0.11760040004828169	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1621	0.1118065973479558	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	1572	0.10842687910609901	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG	1462	0.1008397565223389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36231959029538047	0.0
2	0.0	0.0	0.0	1.4993533702343387	0.0
3	0.0	0.0	0.0	2.4250512992947426	0.0
4	0.0	0.0	0.0	2.9525632403910818	0.0
5	0.0	0.0	0.0	6.041487765764834	0.0
6	0.0	0.0	0.0	6.543134516234718	0.0
7	0.0	0.0	0.0	8.896866863242115	0.0
8	0.0	0.0	0.0	11.355439449588744	0.0
9	0.0	0.0	0.0	13.34064456055041	0.0
10	0.0	0.0	0.0	14.563826668735881	0.0
11	0.0	0.0	0.0	15.694238959874468	0.0
12	0.0	0.0	0.0	16.643181073577846	0.0
13	0.0	0.0	0.0	16.983842877588675	0.0
14	0.0	0.0	0.0	17.26091079957926	0.0
15	6.897384167054644E-5	0.0	0.0	18.286758746745296	0.0
16	6.897384167054644E-5	0.0	0.0	19.512148017864224	0.0
17	6.897384167054644E-5	0.0	0.0	21.0690255720518	0.0
18	6.897384167054644E-5	0.0	0.0	21.756418878140465	0.0
19	6.897384167054644E-5	0.0	0.0	22.56127463659407	0.0
20	6.897384167054644E-5	0.0	0.0	23.62009208007863	0.0
21	6.897384167054644E-5	0.0	0.0	24.57820771472419	0.0
22	1.3794768334109288E-4	0.0	0.0	25.67371924197748	0.0
23	1.3794768334109288E-4	0.0	0.0	26.427465383753212	0.0
24	1.3794768334109288E-4	0.0	0.0	27.099270601624333	0.0
25	1.3794768334109288E-4	0.0	0.0	27.75169416998603	0.0
26	1.3794768334109288E-4	0.0	0.0	28.325073715793284	0.0
27	1.3794768334109288E-4	0.0	0.0	28.929560464193955	0.0
28	1.3794768334109288E-4	0.0	0.0	29.521769868777266	0.0
29	1.3794768334109288E-4	0.0	0.0	30.31738313244702	0.0
30	1.3794768334109288E-4	0.0	0.0	31.0607142241305	0.0
31	2.0692152501163933E-4	0.0	0.0	31.640577311054784	0.0
32	2.0692152501163933E-4	0.0	0.0	32.202438225303055	0.0
33	2.0692152501163933E-4	0.0	0.0	32.76629938095977	0.0
34	2.0692152501163933E-4	0.0	0.0	33.54115151828669	0.0
35	2.0692152501163933E-4	0.0	0.0	34.12929146621144	0.0
36	2.0692152501163933E-4	0.0	0.0	34.70329177659373	0.0
37	2.0692152501163933E-4	0.0	0.0	35.26680806304209	0.0
38	2.0692152501163933E-4	0.0	0.0	35.8068042694808	0.0
39	2.0692152501163933E-4	0.0	0.0	36.39970341248082	0.0
40	2.0692152501163933E-4	0.0	0.0	36.946803924611594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCAA	35	1.0198164E-7	46.000004	9
AGCGTTG	20	6.312003E-4	46.0	30
AGCGTAA	20	6.312003E-4	46.0	14
AATCCGG	85	0.0	46.0	1
ACACCGT	20	6.312003E-4	46.0	13
CCGCTAA	30	1.8617429E-6	46.0	15
CGCTAAA	30	1.8617429E-6	46.0	16
AGTTCGG	30	1.8617429E-6	46.0	1
TTAACGA	25	3.417601E-5	46.0	25
GCGATAC	75	0.0	46.0	8
ACCGTCC	20	6.312003E-4	46.0	11
CGGTCGG	20	6.312003E-4	46.0	1
GATTCCG	25	3.417601E-5	46.0	8
CGATATC	20	6.312003E-4	46.0	9
CGATACG	20	6.312003E-4	46.0	9
AGTACGG	105	0.0	46.0	1
TAGTCGG	30	1.8617429E-6	46.0	1
GCGTAAC	25	3.417601E-5	46.0	15
TCGCGGA	20	6.312003E-4	46.0	2
TTAGCGG	290	0.0	45.206894	1
>>END_MODULE