FastQCFastQC Report
Thu 26 May 2016
SRR1545003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545003_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences897576
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT55700.6205602645347024Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT39660.44185673413727644Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT38120.42469941264026667Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTTG23410.2608135689902582Illumina Single End Adapter 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18990.21156982807026925No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC16110.17948340864729004Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT15690.17480413914810555Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC12510.139375384368566Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC11920.13280212483399734Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT11030.12288652994286835Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC10590.11798443808657985Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGG351.0192343E-746.0000041
CGTTCCG206.310713E-446.019
CCGTAGG750.046.01
TAACCGG253.416553E-546.01
ACTACGG800.046.01
GCGATAG453.092282E-1046.08
ATAACGG750.046.01
ACGTAGG1000.046.01
TCGCGGA206.310713E-446.02
CGTAGGG4350.044.9425282
CACGAGG1700.044.647061
TTTGCGG2550.044.1960751
CTCGCGG1050.043.8095251
CGTTGAT1000.043.724
ACGGGTA1000.043.74
TCGAAGG1000.043.71
ACAGCGG1950.043.6410261
ATAGCGG950.043.5789451
CGTTTTT15650.043.3546331
GCGATGA800.043.1258
Produced by FastQC (version 0.11.5)