FastQCFastQC Report
Thu 26 May 2016
SRR1545002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545002_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences413566
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT23010.5563803600876281Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT18550.4485378391840722Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT16870.4079155443145713Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.26477031477442536No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC7450.18014053379629855Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT6980.16877596320780722Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC5570.13468225144233326Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC5530.1337150539454404Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG5410.13081346145476175No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC4880.11799809462093111Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA4820.1165472983755918No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT4610.11146951151690421Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT4350.10518272778710049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTG700.046.0000044
ACGATGG351.0174517E-746.0000041
ACTACGG351.0174517E-746.0000041
CGCAGTA351.0174517E-746.00000436
CTCACGA301.8584924E-646.023
ACCGGAG253.4133365E-546.041
TTAATCG301.8584924E-646.019
ATCTACG206.3067453E-446.011
CGAGTCT206.3067453E-446.016
CATGCGG800.046.01
CCCTACA750.046.026
GCGCGAC206.3067453E-446.08
GACGTCG206.3067453E-446.018
CGTTGAT301.8584924E-646.024
GATCCGG206.3067453E-446.01
TCGGGCA501.6370905E-1146.04
CGATTCG253.4133365E-546.09
TACGGGT253.4133365E-546.03
GTTACGG206.3067453E-446.01
CTAACGG405.5970304E-946.01
Produced by FastQC (version 0.11.5)