##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545002_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	413566
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.875187999013455	34.0	33.0	34.0	31.0	34.0
2	33.17747348669862	34.0	33.0	34.0	31.0	34.0
3	33.22183641788735	34.0	33.0	34.0	31.0	34.0
4	36.53814143328997	37.0	37.0	37.0	35.0	37.0
5	36.53438387101454	37.0	37.0	37.0	35.0	37.0
6	36.6455874032198	37.0	37.0	37.0	35.0	37.0
7	36.48658738871184	37.0	37.0	37.0	35.0	37.0
8	36.51224230231692	37.0	37.0	37.0	35.0	37.0
9	38.29103939879003	39.0	39.0	39.0	37.0	39.0
10	38.117287204460716	39.0	39.0	39.0	35.0	39.0
11	37.567082400390746	39.0	37.0	39.0	35.0	39.0
12	37.5198904165236	39.0	37.0	39.0	35.0	39.0
13	37.511869931280614	39.0	35.0	39.0	35.0	39.0
14	38.87040762538506	41.0	37.0	41.0	35.0	41.0
15	38.9306930453664	41.0	37.0	41.0	35.0	41.0
16	38.9291527833526	41.0	37.0	41.0	35.0	41.0
17	38.97334161899189	41.0	37.0	41.0	35.0	41.0
18	38.954846384857554	41.0	37.0	41.0	35.0	41.0
19	38.86192772133106	41.0	37.0	41.0	35.0	41.0
20	38.78291493981614	41.0	35.0	41.0	35.0	41.0
21	38.74235793077767	41.0	35.0	41.0	35.0	41.0
22	38.718613232228954	41.0	35.0	41.0	35.0	41.0
23	38.70545692827747	41.0	35.0	41.0	35.0	41.0
24	38.65943041739408	41.0	35.0	41.0	35.0	41.0
25	38.635963788125714	41.0	35.0	41.0	35.0	41.0
26	38.596606103983405	41.0	35.0	41.0	35.0	41.0
27	38.56925134077753	41.0	36.0	41.0	35.0	41.0
28	38.5932450926817	40.0	36.0	41.0	35.0	41.0
29	38.59899024581324	40.0	36.0	41.0	35.0	41.0
30	38.51270172112795	40.0	36.0	41.0	35.0	41.0
31	38.45451511971487	40.0	35.0	41.0	35.0	41.0
32	38.35183985143846	40.0	35.0	41.0	35.0	41.0
33	38.248811556075694	40.0	35.0	41.0	35.0	41.0
34	38.1487477210409	40.0	35.0	41.0	35.0	41.0
35	38.09969388199223	40.0	35.0	41.0	35.0	41.0
36	38.014691729977805	40.0	35.0	41.0	35.0	41.0
37	37.956814631763734	40.0	35.0	41.0	35.0	41.0
38	37.897007974543364	40.0	35.0	41.0	35.0	41.0
39	37.82065015015741	40.0	35.0	41.0	35.0	41.0
40	37.732149161197974	40.0	35.0	41.0	34.0	41.0
41	37.71913551887728	40.0	35.0	41.0	34.0	41.0
42	37.627416180246925	40.0	35.0	41.0	35.0	41.0
43	37.554750632305364	40.0	35.0	41.0	34.0	41.0
44	37.49170628146415	40.0	35.0	41.0	34.0	41.0
45	37.43854668904117	40.0	35.0	41.0	34.0	41.0
46	37.46825174216449	40.0	35.0	41.0	34.0	41.0
47	37.40217039118303	39.0	35.0	41.0	34.0	41.0
48	37.42608193129996	40.0	35.0	41.0	34.0	41.0
49	37.37073163654652	39.0	35.0	41.0	34.0	41.0
50	37.27146573944667	39.0	35.0	41.0	34.0	41.0
51	37.20993988867557	39.0	35.0	41.0	34.0	41.0
52	37.00236721587365	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	6.0
16	9.0
17	12.0
18	34.0
19	53.0
20	88.0
21	159.0
22	289.0
23	503.0
24	845.0
25	1590.0
26	2084.0
27	2501.0
28	2512.0
29	2610.0
30	2863.0
31	3636.0
32	4660.0
33	6228.0
34	17021.0
35	69823.0
36	14412.0
37	22044.0
38	39336.0
39	217979.0
40	2263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44609082951693	23.045414758466606	25.021399244618753	19.487095167397705
2	26.922425924761704	24.334930821199034	26.622594700724918	22.120048553314344
3	24.99020712534396	24.832070334601973	27.315833506622884	22.861889033431183
4	25.52192394926082	27.23966670374257	24.454379712065304	22.784029634931304
5	23.328803625056217	35.65984631231775	21.26964015417128	19.74170990845476
6	85.07420822794911	2.695337624466228	9.17241746178361	3.058036685801057
7	86.02447976864636	2.3459375287136757	6.5148005396962025	5.114782162943762
8	74.3970732603744	5.016128018260689	13.177340497042794	7.409458224322115
9	46.517121813688746	19.36788807590566	17.3084828056465	16.806507304759094
10	37.07509804964625	21.328881000855972	27.845374136171735	13.750646813326048
11	34.538139015296224	18.19274311718081	28.32994008211507	18.93917778540789
12	20.85350343113312	29.907680998921577	30.63984950406948	18.59896606587582
13	14.819883646141124	32.72343471175097	31.75623721485809	20.700444427249824
14	13.019929104423477	24.606471518451713	41.531702315954405	20.841897061170407
15	15.820933055425252	19.333794364140186	43.91826213953758	20.92701044089698
16	16.165980762441787	23.366766126809264	28.49001126785084	31.977241842898113
17	19.034930337600287	23.09474183080814	33.56441293529932	24.30591489629225
18	22.71898560326526	27.12239400724431	29.108775866488056	21.049844523002374
19	23.805631991024406	23.315988258222387	27.381844735785826	25.49653501496738
20	19.13576067665137	29.749786007553812	29.301248168369742	21.813205147425077
21	19.36861347402833	25.226928712708492	27.597529777592932	27.806928035670243
22	17.213455651576773	28.989326975621786	24.9437816454931	28.853435727308334
23	17.063781838932602	23.513780146336984	37.27047194401861	22.151966070711808
24	19.172997780281744	22.67401091965974	34.039548705647945	24.113442594410568
25	15.505868470812398	29.888578848357938	30.216458799804624	24.389093881025037
26	17.40907134532336	27.773076123278994	28.991503169989798	25.826349361407853
27	20.09425339607221	25.864795461909345	34.472369585507515	19.56858155651093
28	16.715107141302717	23.98214553420736	30.964102464902822	28.338644859587102
29	18.220791844590707	25.977957569045813	30.450762393426928	25.350488192936556
30	26.21975694326903	24.546021674895904	23.71108843570313	25.523132946131938
31	23.54690666060556	24.89663076751957	25.388934293438048	26.167528278436812
32	23.551500848715804	25.538124507333777	24.898565162513357	26.01180948143706
33	19.02018057577267	24.779599870395536	26.20331458582185	29.99690496800994
34	18.692300624325984	26.067665136882628	27.723749050937457	27.516285187853935
35	27.20750738697088	21.459694462310733	24.404085442226876	26.92871270849151
36	19.527475662892986	29.080727139078167	25.425929597694203	25.96586760033465
37	20.634916796835327	27.30809592664774	26.718105453543085	25.338881822973843
38	19.795389369532312	28.995130160603143	24.2648090026743	26.944671467190247
39	25.1270655711543	25.010518272778707	26.53071093852009	23.331705217546897
40	17.396981376612196	23.518374334447223	30.891320853261632	28.193323435678945
41	21.43551452488841	25.696503097449984	25.115459201191587	27.752523176470017
42	21.561491998858706	22.198149751188446	28.307936339060753	27.932421910892096
43	22.057180716016305	24.88720059192487	26.984568363937072	26.071050328121753
44	17.1689645667197	28.07024755419933	27.01285889072119	27.747928988359778
45	21.983431906878227	25.21169535213243	26.192917212730254	26.61195552825909
46	23.30873427699569	23.780726655479416	27.06146056494006	25.849078502584838
47	20.829081694336576	20.985525889458998	31.76687638732391	26.418516028880518
48	20.0483115149698	20.36240890208576	27.85238631802421	31.73689326492023
49	20.740583123370875	23.014706237940256	30.79508470232079	25.449625936368076
50	19.861642398069474	24.015755647224385	28.623726321796276	27.498875632909865
51	19.393035210824873	21.78032043253072	29.64750487225739	29.17913948438701
52	20.519820294705077	20.296397672922822	34.681042445462154	24.50273958690995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	145.5
2	214.0
3	1111.0
4	2008.0
5	1359.5
6	711.0
7	775.0
8	839.0
9	838.0
10	837.0
11	865.5
12	894.0
13	839.0
14	804.5
15	825.0
16	737.5
17	650.0
18	609.5
19	569.0
20	621.5
21	674.0
22	858.0
23	1042.0
24	1027.0
25	1012.0
26	1291.5
27	1571.0
28	1971.5
29	2372.0
30	2867.5
31	3363.0
32	3995.5
33	4628.0
34	5641.5
35	6655.0
36	6913.5
37	7172.0
38	8422.5
39	11092.0
40	12511.0
41	16013.5
42	19516.0
43	24131.5
44	28747.0
45	32332.0
46	35917.0
47	38275.0
48	40633.0
49	46185.0
50	51737.0
51	52336.5
52	52936.0
53	46655.5
54	40375.0
55	33466.5
56	26558.0
57	21755.5
58	16953.0
59	14448.0
60	11943.0
61	10995.5
62	10048.0
63	8592.0
64	5740.0
65	4344.0
66	3509.5
67	2675.0
68	2163.0
69	1651.0
70	1404.5
71	1158.0
72	1021.0
73	884.0
74	694.0
75	504.0
76	465.5
77	427.0
78	326.5
79	226.0
80	148.0
81	70.0
82	51.5
83	33.0
84	22.0
85	11.0
86	6.0
87	1.0
88	1.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	413566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.606481593182906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75348087096218	26.266424767496783
2	11.153602649656586	8.165883001846597
3	3.902965471468968	4.286215010704716
4	2.0261425186452477	2.9667979525581005
5	1.3378260804413313	2.4486552894277813
6	1.006959170451474	2.2116739402931165
7	0.8783746359491926	2.2507943459955
8	0.8140001580931592	2.383814544326816
9	0.7261006906025346	2.3921992408805166
>10	6.216153727815609	37.55735842381098
>50	0.13086027645487908	3.2857338457720258
>100	0.04758555507450149	3.357346924216066
>500	0.00330455243572927	0.748550218600887
>1k	0.002643641948583416	1.678552494070121
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	2301	0.5563803600876281	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	1855	0.4485378391840722	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1687	0.4079155443145713	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1095	0.26477031477442536	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	745	0.18014053379629855	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	698	0.16877596320780722	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	557	0.13468225144233326	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	553	0.1337150539454404	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	541	0.13081346145476175	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	488	0.11799809462093111	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	482	0.1165472983755918	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	461	0.11146951151690421	Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	435	0.10518272778710049	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4548246229138759	0.0
2	0.0	0.0	0.0	1.72064434697243	0.0
3	0.0	0.0	0.0	2.741763104317086	0.0
4	0.0	0.0	0.0	3.3938960165971093	0.0
5	0.0	0.0	0.0	6.938433043335284	0.0
6	0.0	0.0	0.0	7.488768419067331	0.0
7	0.0	0.0	0.0	9.92804050623117	0.0
8	0.0	0.0	0.0	12.600165390771968	0.0
9	0.0	0.0	0.0	14.859296944139508	0.0
10	0.0	0.0	0.0	16.290265640792523	0.0
11	2.417993742232195E-4	0.0	0.0	17.576154712911602	0.0
12	2.417993742232195E-4	0.0	0.0	18.64998573383692	0.0
13	2.417993742232195E-4	0.0	0.0	19.089576996174735	0.0
14	2.417993742232195E-4	0.0	0.0	19.440427888172625	0.0
15	2.417993742232195E-4	0.0	0.0	20.65885493488343	0.0
16	2.417993742232195E-4	0.0	0.0	21.96578055255993	0.0
17	2.417993742232195E-4	0.0	0.0	23.586078159229725	0.0
18	2.417993742232195E-4	0.0	0.0	24.361286952989367	0.0
19	2.417993742232195E-4	0.0	0.0	25.392319484677174	0.0
20	2.417993742232195E-4	0.0	0.0	26.5599686628011	0.0
21	2.417993742232195E-4	0.0	0.0	27.576009633287068	0.0
22	2.417993742232195E-4	0.0	0.0	28.689253952210773	0.0
23	2.417993742232195E-4	0.0	0.0	29.481630501540263	0.0
24	2.417993742232195E-4	0.0	0.0	30.199049244860554	0.0
25	2.417993742232195E-4	0.0	0.0	30.919611380045747	0.0
26	2.417993742232195E-4	0.0	0.0	31.608255997833478	0.0
27	2.417993742232195E-4	0.0	0.0	32.30004400748611	0.0
28	2.417993742232195E-4	0.0	0.0	32.926062587350025	0.0
29	2.417993742232195E-4	0.0	0.0	33.723275124163976	0.0
30	2.417993742232195E-4	0.0	0.0	34.470435190513726	0.0
31	2.417993742232195E-4	0.0	0.0	35.11313792719904	0.0
32	2.417993742232195E-4	0.0	0.0	35.77929520318401	0.0
33	2.417993742232195E-4	0.0	0.0	36.4299773192187	0.0
34	2.417993742232195E-4	0.0	0.0	37.22259566792241	0.0
35	2.417993742232195E-4	0.0	0.0	37.85490103151613	0.0
36	2.417993742232195E-4	0.0	0.0	38.48237040762538	0.0
37	2.417993742232195E-4	0.0	0.0	39.07985666133096	0.0
38	2.417993742232195E-4	0.0	0.0	39.63672062016703	0.0
39	2.417993742232195E-4	0.0	0.0	40.287886334950166	0.0
40	2.417993742232195E-4	0.0	0.0	40.88875777989487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTG	70	0.0	46.000004	4
ACGATGG	35	1.0174517E-7	46.000004	1
ACTACGG	35	1.0174517E-7	46.000004	1
CGCAGTA	35	1.0174517E-7	46.000004	36
CTCACGA	30	1.8584924E-6	46.0	23
ACCGGAG	25	3.4133365E-5	46.0	41
TTAATCG	30	1.8584924E-6	46.0	19
ATCTACG	20	6.3067453E-4	46.0	11
CGAGTCT	20	6.3067453E-4	46.0	16
CATGCGG	80	0.0	46.0	1
CCCTACA	75	0.0	46.0	26
GCGCGAC	20	6.3067453E-4	46.0	8
GACGTCG	20	6.3067453E-4	46.0	18
CGTTGAT	30	1.8584924E-6	46.0	24
GATCCGG	20	6.3067453E-4	46.0	1
TCGGGCA	50	1.6370905E-11	46.0	4
CGATTCG	25	3.4133365E-5	46.0	9
TACGGGT	25	3.4133365E-5	46.0	3
GTTACGG	20	6.3067453E-4	46.0	1
CTAACGG	40	5.5970304E-9	46.0	1
>>END_MODULE