FastQCFastQC Report
Thu 26 May 2016
SRR1545001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545001_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences270958
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT18190.6713217546630843Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT15190.5606034883635101Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT13720.5063515378767189Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTTG9050.33400010333704855Illumina Single End Adapter 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.28233157906391393No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT5520.20372160999121638Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC5150.1900663571476022Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC4440.16386303412336967Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC4240.15648181637006472Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC4080.1505768421674208Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA3710.13692158932380663No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT3500.12917131068283647Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGG206.3028745E-446.0000041
CCGGGTC206.3028745E-446.0000044
CACGGGA206.3028745E-446.0000043
CACGGAA206.3028745E-446.0000043
ATTCGAA405.5842975E-946.00000441
TCTCGAG206.3028745E-446.00000413
GCGGGCC206.3028745E-446.0000044
TAGGGCG405.5842975E-946.0000044
ACATCGG206.3028745E-446.0000041
TGAATGG206.3028745E-446.0000041
TCGAATT405.5842975E-946.00000430
TGCGTCT206.3028745E-446.00000417
ATTTAGG301.8561022E-646.01
GGCACCG1000.046.07
TTATGAC600.046.026
GACCGAT301.8561022E-646.08
ACCGATC253.4101977E-546.010
TTCACGG253.4101977E-546.01
CACGCGG453.074092E-1046.01
AAGGTGA501.6370905E-1146.015
Produced by FastQC (version 0.11.5)