##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545001_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	270958
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90232434547052	34.0	33.0	34.0	31.0	34.0
2	33.2538326973184	34.0	33.0	34.0	31.0	34.0
3	33.374984314912275	34.0	34.0	34.0	31.0	34.0
4	36.619479771772745	37.0	37.0	37.0	35.0	37.0
5	36.62252083348711	37.0	37.0	37.0	35.0	37.0
6	36.401287284376174	37.0	37.0	37.0	35.0	37.0
7	36.2568516153795	37.0	37.0	37.0	35.0	37.0
8	36.3899017559917	37.0	37.0	37.0	35.0	37.0
9	38.267724149130125	39.0	39.0	39.0	37.0	39.0
10	38.18245632164395	39.0	39.0	39.0	35.0	39.0
11	37.70534547789694	39.0	37.0	39.0	35.0	39.0
12	37.72274669875036	39.0	37.0	39.0	35.0	39.0
13	37.72057662073089	39.0	37.0	39.0	35.0	39.0
14	39.04379645553924	41.0	38.0	41.0	35.0	41.0
15	39.07541021117664	41.0	38.0	41.0	35.0	41.0
16	39.10356586629662	41.0	38.0	41.0	35.0	41.0
17	39.168022350327355	41.0	38.0	41.0	35.0	41.0
18	39.17122579883229	41.0	38.0	41.0	35.0	41.0
19	39.10378361222035	41.0	37.0	41.0	35.0	41.0
20	39.03820149248223	41.0	37.0	41.0	35.0	41.0
21	39.00200400062002	41.0	37.0	41.0	35.0	41.0
22	38.98041024808273	41.0	37.0	41.0	35.0	41.0
23	38.946445574590896	41.0	37.0	41.0	35.0	41.0
24	38.9210578761284	41.0	36.0	41.0	35.0	41.0
25	38.89634555909034	41.0	36.0	41.0	35.0	41.0
26	38.85429476154976	41.0	36.0	41.0	35.0	41.0
27	38.92018689243351	41.0	36.0	41.0	35.0	41.0
28	38.9284095690107	41.0	36.0	41.0	35.0	41.0
29	38.93137312793865	41.0	36.0	41.0	35.0	41.0
30	38.86658817971789	41.0	36.0	41.0	35.0	41.0
31	38.80709556462625	41.0	36.0	41.0	35.0	41.0
32	38.725739782549326	41.0	36.0	41.0	35.0	41.0
33	38.65059160460293	41.0	36.0	41.0	35.0	41.0
34	38.53750396740454	41.0	36.0	41.0	35.0	41.0
35	38.480037496586185	41.0	36.0	41.0	35.0	41.0
36	38.435270410912395	41.0	36.0	41.0	35.0	41.0
37	38.38890898220389	41.0	35.0	41.0	35.0	41.0
38	38.34692461562309	40.0	36.0	41.0	35.0	41.0
39	38.30078831405605	40.0	36.0	41.0	35.0	41.0
40	38.255519305575035	40.0	35.0	41.0	35.0	41.0
41	38.230866038279	40.0	35.0	41.0	35.0	41.0
42	38.168321289646364	40.0	35.0	41.0	35.0	41.0
43	38.104898176101095	40.0	35.0	41.0	35.0	41.0
44	37.983270469962136	40.0	35.0	41.0	35.0	41.0
45	37.98110408255154	40.0	35.0	41.0	35.0	41.0
46	37.934440023915144	40.0	35.0	41.0	35.0	41.0
47	37.851585854634294	40.0	35.0	41.0	35.0	41.0
48	37.82095011034921	40.0	35.0	41.0	35.0	41.0
49	37.77510167627455	40.0	35.0	41.0	35.0	41.0
50	37.69562072350696	40.0	35.0	41.0	35.0	41.0
51	37.62797186279793	40.0	35.0	41.0	35.0	41.0
52	37.18602144981879	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	4.0
17	4.0
18	13.0
19	25.0
20	47.0
21	87.0
22	127.0
23	253.0
24	477.0
25	830.0
26	1201.0
27	1332.0
28	1198.0
29	1329.0
30	1330.0
31	1562.0
32	2170.0
33	3104.0
34	9803.0
35	42262.0
36	8282.0
37	13443.0
38	25292.0
39	154842.0
40	1938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.15553702049764	22.84708331180478	24.371673838749917	20.62570582894766
2	27.013780733545424	24.12145055691288	26.370138545457227	22.49463016408447
3	24.920836439595803	24.737044117538513	27.378043829671018	22.964075613194666
4	25.751961558617943	26.865049195816326	24.514131341388705	22.86885790417703
5	23.454188472014113	34.852633987555265	21.604455303035895	20.088722237394727
6	83.72109330597361	2.501494696595044	9.962429601635678	3.814982395795658
7	84.63230463761911	2.2401995881280494	7.179710508639715	5.947785265613121
8	72.02260128876063	4.987119775020483	14.610013360004134	8.380265576214764
9	45.20073221680113	18.71323230906635	17.94521660183497	18.14081887229755
10	35.60404195484171	20.78181858443006	29.584289816133865	14.029849644594366
11	32.99478147904841	17.857011049682978	28.886764738446548	20.26144273282206
12	20.41755548830446	28.039400940367145	31.893503790255316	19.64953978107308
13	14.760221141283889	30.91253995084109	32.8504786719713	21.47676023590372
14	13.016777507953261	24.231799762324787	40.54613630156703	22.205286428154917
15	15.482473298444777	19.165700957343944	43.579447737287694	21.77237800692358
16	15.921286693878756	23.78302172292385	29.365436709748376	30.93025487344902
17	18.6043593472051	23.010577285040487	33.697473409163045	24.687589958591367
18	22.001933879051368	27.562574273503643	29.665483211420216	20.770008636024773
19	23.087711010562522	23.031244694749738	27.75854560485389	26.12249868983385
20	18.627610183128013	30.401390621424724	28.890086286435533	22.08091290901173
21	18.665623454557533	25.152606677049576	27.866680444939806	28.31508942345308
22	16.8882262195617	29.129606802530283	24.918622074269813	29.0635449036382
23	16.504771957277512	23.22721602609999	37.14819270883311	23.1198193077894
24	19.314432495073035	22.802426944397286	33.50297832136346	24.380162239166218
25	15.438185991924946	29.238110703503867	30.5981000745503	24.72560323002089
26	17.254703681013297	26.304814768340478	29.382413510580975	27.058068040065248
27	21.009160091231852	24.74331815262882	34.126691221517724	20.120830534621604
28	17.146568840927376	23.38554314690838	30.218336421142762	29.24955159102149
29	18.42757918201345	25.24819344695488	30.063330848323353	26.260896522708315
30	26.530680031591615	23.85867920489522	23.004672310837844	26.60596845267532
31	23.2493596793599	24.279408616833607	24.95368285859801	27.517548845208484
32	23.276301124159463	24.53848935997461	25.010149174410795	27.175060341455133
33	19.05793517814569	23.841333343174956	26.207382693996855	30.893348784682495
34	19.088567231821905	25.352637678164143	27.42454550151684	28.134249588497113
35	26.724806058503532	20.88478657208866	24.269443972866643	28.12096339654116
36	19.381601576628114	29.180168144140424	24.22626384900981	27.211966430221658
37	20.82315340384857	26.912658050325145	26.661327585825113	25.602860960001184
38	19.934823847238317	28.73655695716679	23.25969338421453	28.068925811380364
39	25.596217864023206	24.793510433351294	26.186715284287605	23.4235564183379
40	17.66436126632172	23.06003144398763	30.70438961019789	28.57121767949276
41	21.76093711940596	25.60064659467519	24.544763395064916	28.09365289085393
42	21.37748285712177	21.27008613881118	28.605909402933293	28.746521601133757
43	22.861845747311392	23.871965396851174	26.91044368499915	26.355745170838286
44	17.255810863676288	26.537323127569586	27.76814118793319	28.438724820820937
45	21.730305065729745	24.21925169214417	26.566109876807474	27.48433336531861
46	23.664922238870968	22.559584880313555	27.24075317945955	26.53473970135593
47	20.54266712922298	20.005683537670045	32.371806700669474	27.0798426324375
48	19.792735405487196	19.39267340325807	27.588777596527876	33.22581359472686
49	20.731626303707586	21.522892846861875	31.26425497678607	26.48122587264447
50	20.22269133961721	22.289432310542594	29.48648868090257	28.00138766893762
51	19.27383579742986	20.464057160150283	30.153381704913677	30.108725337506183
52	20.31717092685951	19.266085518788888	35.68449722835273	24.732246325998865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	95.5
2	145.0
3	765.5
4	1386.0
5	926.5
6	467.0
7	493.5
8	520.0
9	536.0
10	552.0
11	532.5
12	513.0
13	521.5
14	501.0
15	472.0
16	445.0
17	418.0
18	395.5
19	373.0
20	371.5
21	370.0
22	531.5
23	693.0
24	638.5
25	584.0
26	735.0
27	886.0
28	1111.0
29	1336.0
30	1594.0
31	1852.0
32	2160.0
33	2468.0
34	3057.0
35	3646.0
36	3923.0
37	4200.0
38	4923.5
39	6379.5
40	7112.0
41	9379.0
42	11646.0
43	14330.5
44	17015.0
45	19280.0
46	21545.0
47	23820.5
48	26096.0
49	31130.5
50	36165.0
51	37105.0
52	38045.0
53	33535.5
54	29026.0
55	23948.0
56	18870.0
57	15172.5
58	11475.0
59	9585.0
60	7695.0
61	7060.5
62	6426.0
63	5601.0
64	3798.5
65	2821.0
66	2291.5
67	1762.0
68	1460.0
69	1158.0
70	966.5
71	775.0
72	674.5
73	574.0
74	468.0
75	362.0
76	320.0
77	278.0
78	209.0
79	140.0
80	97.5
81	55.0
82	42.0
83	29.0
84	17.5
85	6.0
86	3.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	270958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.307698013962174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38230987386177	26.87951186018942
2	12.060273687685548	9.481231921625549
3	4.651262530394085	5.484912687851647
4	2.697528154114387	4.241344882643565
5	2.0926700408359524	4.112902100402275
6	1.8304392195993842	4.31702112461551
7	1.568479228877815	4.315731550893098
8	1.4200893439784508	4.465635446876048
9	1.134277970068388	4.0127270320204405
>10	4.006320865140062	23.53772637566155
>50	0.09759369664669278	2.5334469360703964
>100	0.05178441046559208	3.7027000260027627
>500	0.003983416189660929	1.0713912645279164
>1k	0.002987562142245697	1.8437167906198342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1819	0.6713217546630843	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	1519	0.5606034883635101	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1372	0.5063515378767189	Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTTG	905	0.33400010333704855	Illumina Single End Adapter 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.28233157906391393	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	552	0.20372160999121638	Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	515	0.1900663571476022	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	444	0.16386303412336967	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	424	0.15648181637006472	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	408	0.1505768421674208	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	371	0.13692158932380663	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	350	0.12917131068283647	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5676156452291499	0.0
2	0.0	0.0	0.0	2.0995873899275903	0.0
3	0.0	0.0	0.0	3.3012496401656346	0.0
4	0.0	0.0	0.0	4.009477483595243	0.0
5	0.0	0.0	0.0	8.091659962060541	0.0
6	0.0	0.0	0.0	8.721277836417451	0.0
7	0.0	0.0	0.0	11.567844462979503	0.0
8	0.0	0.0	0.0	14.733648757371991	0.0
9	0.0	0.0	0.0	17.5126772414913	0.0
10	0.0	0.0	0.0	19.20260704611047	0.0
11	0.0	0.0	0.0	20.734947851696575	0.0
12	0.0	0.0	0.0	21.974992434251803	0.0
13	0.0	0.0	0.0	22.518619121782713	0.0
14	0.0	0.0	0.0	22.929014828866467	0.0
15	0.0	0.0	0.0	24.313731279386474	0.0
16	0.0	0.0	0.0	25.77779582075451	0.0
17	0.0	0.0	0.0	27.531942219827428	0.0
18	0.0	0.0	0.0	28.405878401818732	0.0
19	0.0	0.0	0.0	29.492024594217554	0.0
20	0.0	0.0	0.0	30.750522221156046	0.0
21	0.0	0.0	0.0	31.8809557200747	0.0
22	0.0	0.0	0.0	33.06601023036781	0.0
23	0.0	0.0	0.0	33.910052480458226	0.0
24	0.0	0.0	0.0	34.69024719698256	0.0
25	0.0	0.0	0.0	35.45900102598927	0.0
26	0.0	0.0	0.0	36.18974158356646	0.0
27	0.0	0.0	0.0	36.910517497176684	0.0
28	0.0	0.0	0.0	37.57224366876047	0.0
29	0.0	0.0	0.0	38.363141151027094	0.0
30	0.0	0.0	0.0	39.13226404092147	0.0
31	0.0	0.0	0.0	39.81761010931584	0.0
32	0.0	0.0	0.0	40.49262247285557	0.0
33	0.0	0.0	0.0	41.12408565165081	0.0
34	0.0	0.0	0.0	41.92457871699673	0.0
35	3.69060887665247E-4	0.0	0.0	42.56010156555629	0.0
36	3.69060887665247E-4	0.0	0.0	43.18455258748588	0.0
37	3.69060887665247E-4	0.0	0.0	43.79461023479654	0.0
38	3.69060887665247E-4	0.0	0.0	44.35447560138471	0.0
39	3.69060887665247E-4	0.0	0.0	44.9471873869751	0.0
40	3.69060887665247E-4	0.0	0.0	45.53399419836285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGG	20	6.3028745E-4	46.000004	1
CCGGGTC	20	6.3028745E-4	46.000004	4
CACGGGA	20	6.3028745E-4	46.000004	3
CACGGAA	20	6.3028745E-4	46.000004	3
ATTCGAA	40	5.5842975E-9	46.000004	41
TCTCGAG	20	6.3028745E-4	46.000004	13
GCGGGCC	20	6.3028745E-4	46.000004	4
TAGGGCG	40	5.5842975E-9	46.000004	4
ACATCGG	20	6.3028745E-4	46.000004	1
TGAATGG	20	6.3028745E-4	46.000004	1
TCGAATT	40	5.5842975E-9	46.000004	30
TGCGTCT	20	6.3028745E-4	46.000004	17
ATTTAGG	30	1.8561022E-6	46.0	1
GGCACCG	100	0.0	46.0	7
TTATGAC	60	0.0	46.0	26
GACCGAT	30	1.8561022E-6	46.0	8
ACCGATC	25	3.4101977E-5	46.0	10
TTCACGG	25	3.4101977E-5	46.0	1
CACGCGG	45	3.074092E-10	46.0	1
AAGGTGA	50	1.6370905E-11	46.0	15
>>END_MODULE