FastQCFastQC Report
Thu 26 May 2016
SRR1545000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545000_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences683718
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT30570.4471141611015067TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT25330.37047437686297563TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT22900.33493340821800804TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.17931369365732655No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT10830.15839863803497933TruSeq Adapter, Index 19 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC10700.1564972693420387TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC7950.11627600852983247TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC7870.11510593548802284TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT7340.107354201586034TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT551.8189894E-1246.0000043
TGATTCA551.8189894E-1246.00000444
TCGCCGA206.309652E-446.020
AACGTCA206.309652E-446.044
CGGGTAT950.046.05
AGACTCG206.309652E-446.032
TAATAGG600.046.01
CGGAAGT206.309652E-446.020
AATCCGG253.4156925E-546.01
CTGTCGG501.6370905E-1146.01
CAAACGG453.092282E-1046.01
CGAGTAG206.309652E-446.020
CATGCGC206.309652E-446.05
GCGAATA206.309652E-446.030
TTAGCCG301.8602896E-646.016
GTGGGCA501.6370905E-1146.04
TAGCGCT206.309652E-446.02
TAGCGAA206.309652E-446.028
ATTACCG206.309652E-446.025
TATATCG206.309652E-446.037
Produced by FastQC (version 0.11.5)