##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545000_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	683718
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94669585998906	34.0	33.0	34.0	31.0	34.0
2	33.22126519998011	34.0	33.0	34.0	31.0	34.0
3	33.261299249105626	34.0	34.0	34.0	31.0	34.0
4	36.553558630897534	37.0	37.0	37.0	35.0	37.0
5	36.54463536136243	37.0	37.0	37.0	35.0	37.0
6	36.667011838214	37.0	37.0	37.0	35.0	37.0
7	36.50516587247959	37.0	37.0	37.0	35.0	37.0
8	36.54376804472019	37.0	37.0	37.0	35.0	37.0
9	38.31553652236741	39.0	39.0	39.0	37.0	39.0
10	38.07599331888293	39.0	39.0	39.0	35.0	39.0
11	37.09558911715064	39.0	35.0	39.0	35.0	39.0
12	36.93837079029658	39.0	35.0	39.0	35.0	39.0
13	36.907625658531735	39.0	35.0	39.0	35.0	39.0
14	38.08375090315013	40.0	35.0	41.0	35.0	41.0
15	38.18101907511576	40.0	35.0	41.0	35.0	41.0
16	38.2250094337139	40.0	35.0	41.0	35.0	41.0
17	38.31437961264732	40.0	36.0	41.0	35.0	41.0
18	38.3161756747665	40.0	36.0	41.0	35.0	41.0
19	38.19627243980705	40.0	35.0	41.0	35.0	41.0
20	38.08928973641179	40.0	35.0	41.0	35.0	41.0
21	38.03529086553228	40.0	35.0	41.0	35.0	41.0
22	38.01754963303584	40.0	35.0	41.0	35.0	41.0
23	38.00943371389959	40.0	35.0	41.0	35.0	41.0
24	37.973913221532854	40.0	35.0	41.0	35.0	41.0
25	37.94513088729564	40.0	35.0	41.0	35.0	41.0
26	37.89315039241325	40.0	35.0	41.0	35.0	41.0
27	37.93270471159162	40.0	35.0	41.0	35.0	41.0
28	37.96969949599104	40.0	35.0	41.0	35.0	41.0
29	37.947617292509484	40.0	35.0	41.0	35.0	41.0
30	37.852959846018386	40.0	35.0	41.0	35.0	41.0
31	37.770155239440825	40.0	35.0	41.0	35.0	41.0
32	37.65664206588096	39.0	35.0	41.0	35.0	41.0
33	37.59952641293633	39.0	35.0	41.0	35.0	41.0
34	37.46331089718276	39.0	35.0	41.0	35.0	41.0
35	37.43370366145107	39.0	35.0	41.0	35.0	41.0
36	37.369083452534525	39.0	35.0	41.0	35.0	41.0
37	37.34974799551862	39.0	35.0	41.0	35.0	41.0
38	37.33199506229176	39.0	35.0	41.0	35.0	41.0
39	37.244429721025334	39.0	35.0	41.0	34.0	41.0
40	37.23098265659234	39.0	35.0	41.0	34.0	41.0
41	37.19554845711244	38.0	35.0	41.0	34.0	41.0
42	37.08362219511553	38.0	35.0	41.0	34.0	41.0
43	37.005853290391656	38.0	35.0	41.0	34.0	41.0
44	36.92052863900029	38.0	35.0	41.0	34.0	41.0
45	36.91283102097648	38.0	35.0	41.0	34.0	41.0
46	36.94596017656402	38.0	35.0	41.0	34.0	41.0
47	36.92526889741092	38.0	35.0	41.0	34.0	41.0
48	36.977888544692405	37.0	35.0	41.0	34.0	41.0
49	36.896221834147994	37.0	35.0	41.0	34.0	41.0
50	36.82543095252721	37.0	35.0	41.0	34.0	41.0
51	36.75879383020485	37.0	35.0	41.0	34.0	41.0
52	36.597382254087215	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	4.0
14	14.0
15	13.0
16	9.0
17	31.0
18	51.0
19	96.0
20	170.0
21	251.0
22	460.0
23	797.0
24	1354.0
25	2297.0
26	3201.0
27	3711.0
28	3641.0
29	3895.0
30	4644.0
31	5850.0
32	7923.0
33	11884.0
34	36606.0
35	190429.0
36	21635.0
37	31793.0
38	56430.0
39	294467.0
40	2059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.775553663937472	22.4877800496696	25.69407270248845	21.042593583904477
2	25.563024521805772	24.02730365443063	28.004966960062482	22.404704863701117
3	25.54050061575094	25.31233637259806	27.043751956215868	22.103411055435135
4	26.09921049321504	28.449594715950145	23.698659388812345	21.75253540202247
5	23.26207588508713	35.02613650657142	20.830225326816027	20.881562281525422
6	87.08912153841204	2.4169321269880273	7.479838178898318	3.01410815570162
7	86.98059726378419	1.992341871941356	5.492469117384653	5.5345917468898
8	77.15988170561548	3.767196417236346	11.983303057693377	7.089618819454804
9	55.05866453713373	15.242541515654114	14.638052530429214	15.060741416782944
10	48.619313810664636	17.026317867892903	23.40643364662039	10.947934674822076
11	45.11611512348658	14.816342410174954	23.276701798109748	16.790840668228714
12	19.85438440994679	37.81383552868288	27.103133163087705	15.228646898282625
13	11.796530148394513	42.7200980521209	27.446842119119285	18.036529680365295
14	10.41789158688231	21.52100719887439	52.17648211689615	15.884619097347151
15	11.96428937076397	15.70823058629435	52.744260060434264	19.58321998250741
16	12.62859834025139	19.12762279185278	28.503125557612936	39.740653310282894
17	16.441281346988085	21.99225411646322	37.727981419240095	23.8384831173086
18	24.679473116109275	26.281156851216437	28.442720536829512	20.59664949584478
19	27.455178889542182	21.71450802816366	25.426418494174495	25.403894588119663
20	17.22113502935421	33.07796489195838	27.418321588725174	22.282578489962233
21	18.80746155578762	23.874901640734922	24.66777238569118	32.649864417786276
22	15.626910509888582	32.910936965240055	21.426816319008715	30.03533620586265
23	15.888275575602806	21.964172363459788	39.97335158647278	22.17420047446462
24	16.373855887953805	21.255985654904507	37.993295481470426	24.37686297567126
25	13.556466262406431	31.949575702263218	30.776723736979285	23.717234298351077
26	16.620448781515186	32.00486165348872	26.90948607466821	24.465203490327884
27	17.257407293650306	27.61621019192123	38.73088027520118	16.395502239227284
28	15.653090894199071	24.709456237805643	34.13454084871248	25.502912019282803
29	18.009618000403677	31.45873006122407	28.226110764964503	22.30554117340775
30	27.935055095814356	24.309876294027653	23.84886751555466	23.90620109460333
31	32.15185207936606	25.65911677036439	22.12125466932273	20.067776480946822
32	24.445165989486895	27.02473826928646	26.931278685071913	21.598817056154733
33	21.943549826097893	25.618895509552186	28.62759207743543	23.809962586914487
34	18.44985798238455	21.766722537654413	34.10031621223955	25.683103267721485
35	33.37969747761504	18.047937892522942	30.468263231332216	18.104101398529803
36	23.01489795500484	24.580163166685683	31.407685624775127	20.99725325353435
37	21.862229749692126	26.327520995498144	28.468462143749324	23.34178711106041
38	18.05949236381081	25.324475880406833	31.0709971069944	25.54503464878795
39	24.89154885493727	20.07172547746293	32.2090393992845	22.8276862683153
40	15.081802731535515	18.218183520106244	34.43671221175982	32.26330153659842
41	21.448023892891513	23.208106266033656	26.59970923684911	28.744160604225723
42	21.149362748969605	19.81664955434843	31.478913821195288	27.55507387548668
43	24.000391974469007	25.875141505708495	29.863481727846864	20.260984791975638
44	15.854489716520554	31.62049265925425	28.13411377205222	24.390903852172972
45	23.414624157913057	27.875673888942515	27.073588818782014	21.63611313436241
46	23.30478355111318	21.455775626793503	29.07119601941151	26.168244802681805
47	22.93547924729201	17.332730745716802	34.44636531435475	25.28542469263644
48	21.548357656226692	15.333953472045492	32.359832562547716	30.7578563091801
49	20.895310639766688	23.6442509923682	31.785326699019183	23.67511166884593
50	19.70446880146493	25.878651724833922	27.952606191441497	26.464273282259644
51	18.656960910784857	19.545777645169498	33.98550279501198	27.811758649033663
52	22.494946747050683	17.769167990311797	36.02186866515142	23.714016597486097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	142.0
1	174.5
2	207.0
3	1135.0
4	2063.0
5	1444.5
6	826.0
7	988.5
8	1151.0
9	1219.5
10	1288.0
11	1221.0
12	1154.0
13	1121.0
14	992.5
15	897.0
16	847.5
17	798.0
18	740.0
19	682.0
20	715.5
21	749.0
22	843.0
23	937.0
24	1103.0
25	1269.0
26	1397.0
27	1525.0
28	2141.0
29	2757.0
30	2953.5
31	3150.0
32	3800.5
33	4451.0
34	5054.5
35	5658.0
36	7395.0
37	9132.0
38	10714.0
39	15823.0
40	19350.0
41	31867.0
42	44384.0
43	65264.0
44	86144.0
45	99622.5
46	113101.0
47	108084.0
48	103067.0
49	86970.0
50	70873.0
51	59563.0
52	48253.0
53	41806.5
54	35360.0
55	29446.0
56	23532.0
57	21583.5
58	19635.0
59	18832.5
60	18030.0
61	17275.5
62	16521.0
63	14277.5
64	10190.5
65	8347.0
66	6372.5
67	4398.0
68	3450.0
69	2502.0
70	2345.0
71	2188.0
72	1844.5
73	1501.0
74	1166.5
75	832.0
76	840.5
77	849.0
78	611.0
79	373.0
80	234.5
81	96.0
82	61.5
83	27.0
84	36.5
85	46.0
86	48.5
87	51.0
88	27.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	683718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.221134255044255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.5568902740477	15.011907286329077
2	13.172415083488012	5.854120080667142
3	5.370702265095683	3.5802928822968436
4	2.7319014539698054	2.428237959208546
5	1.6100815064311644	1.7888918657985406
6	1.0214036534632862	1.3618048627320227
7	0.6748009859068397	1.0496390312290478
8	0.5438224498876558	0.9667481335888541
9	0.36307142825450384	0.7261073056272598
>10	4.784200353812316	27.7001230576267
>50	1.8633416260837266	27.152083621021006
>100	0.30011343004180374	10.207754106762724
>500	0.003297949780679162	0.5220765328205732
>1k	0.003957539736814994	1.6502132742916436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	3057	0.4471141611015067	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	2533	0.37047437686297563	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2290	0.33493340821800804	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1226	0.17931369365732655	No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	1083	0.15839863803497933	TruSeq Adapter, Index 19 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1070	0.1564972693420387	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	795	0.11627600852983247	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	787	0.11510593548802284	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	734	0.107354201586034	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3764710012022501	0.0
2	0.0	0.0	0.0	1.4424075422908276	0.0
3	0.0	0.0	0.0	2.325666429726876	0.0
4	0.0	0.0	0.0	2.8789647193726067	0.0
5	0.0	0.0	0.0	5.8813721446561305	0.0
6	0.0	0.0	0.0	6.529446350688442	0.0
7	0.0	0.0	0.0	9.889457349375034	0.0
8	0.0	0.0	0.0	13.297002565385144	0.0
9	0.0	0.0	0.0	16.92481403151592	0.0
10	0.0	0.0	0.0	18.146077768904725	0.0
11	1.4625913022620437E-4	0.0	0.0	19.418385942742475	0.0
12	1.4625913022620437E-4	0.0	0.0	20.481251042096304	0.0
13	1.4625913022620437E-4	0.0	0.0	20.836221951155302	0.0
14	1.4625913022620437E-4	0.0	0.0	21.124937474221827	0.0
15	1.4625913022620437E-4	0.0	0.0	22.114526749332327	0.0
16	1.4625913022620437E-4	0.0	0.0	23.180609549551132	0.0
17	1.4625913022620437E-4	0.0	0.0	25.00797112259733	0.0
18	1.4625913022620437E-4	0.0	0.0	25.625330911282138	0.0
19	1.4625913022620437E-4	0.0	0.0	26.484310783100636	0.0
20	1.4625913022620437E-4	0.0	0.0	27.36113426880673	0.0
21	1.4625913022620437E-4	0.0	0.0	28.064786944325	0.0
22	1.4625913022620437E-4	0.0	0.0	28.947022017849463	0.0
23	1.4625913022620437E-4	0.0	0.0	29.533082352665865	0.0
24	1.4625913022620437E-4	0.0	0.0	29.98107406854873	0.0
25	1.4625913022620437E-4	0.0	0.0	30.49590620694497	0.0
26	1.4625913022620437E-4	0.0	0.0	30.900605220280877	0.0
27	1.4625913022620437E-4	0.0	0.0	31.32285532924393	0.0
28	2.9251826045240874E-4	0.0	0.0	31.71819375824536	0.0
29	2.9251826045240874E-4	0.0	0.0	32.34930190517143	0.0
30	2.9251826045240874E-4	0.0	0.0	32.94267519649914	0.0
31	2.9251826045240874E-4	0.0	0.0	33.3894968393402	0.0
32	2.9251826045240874E-4	0.0	0.0	33.839389923916	0.0
33	2.9251826045240874E-4	0.0	0.0	34.285187752845474	0.0
34	2.9251826045240874E-4	0.0	0.0	34.95754097449533	0.0
35	2.9251826045240874E-4	0.0	0.0	35.442536250325425	0.0
36	2.9251826045240874E-4	0.0	0.0	35.91363690878403	0.0
37	4.387773906786131E-4	0.0	0.0	36.31906721777107	0.0
38	4.387773906786131E-4	0.0	0.0	36.71411312851205	0.0
39	4.387773906786131E-4	0.0	0.0	37.21285676258341	0.0
40	4.387773906786131E-4	0.0	0.0	37.67167165410301	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	55	1.8189894E-12	46.000004	3
TGATTCA	55	1.8189894E-12	46.000004	44
TCGCCGA	20	6.309652E-4	46.0	20
AACGTCA	20	6.309652E-4	46.0	44
CGGGTAT	95	0.0	46.0	5
AGACTCG	20	6.309652E-4	46.0	32
TAATAGG	60	0.0	46.0	1
CGGAAGT	20	6.309652E-4	46.0	20
AATCCGG	25	3.4156925E-5	46.0	1
CTGTCGG	50	1.6370905E-11	46.0	1
CAAACGG	45	3.092282E-10	46.0	1
CGAGTAG	20	6.309652E-4	46.0	20
CATGCGC	20	6.309652E-4	46.0	5
GCGAATA	20	6.309652E-4	46.0	30
TTAGCCG	30	1.8602896E-6	46.0	16
GTGGGCA	50	1.6370905E-11	46.0	4
TAGCGCT	20	6.309652E-4	46.0	2
TAGCGAA	20	6.309652E-4	46.0	28
ATTACCG	20	6.309652E-4	46.0	25
TATATCG	20	6.309652E-4	46.0	37
>>END_MODULE