FastQCFastQC Report
Thu 26 May 2016
SRR1544999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544999_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences379949
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT22290.5866576830048242TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT17470.45979855191091434TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT14600.3842620983342501TruSeq Adapter, Index 19 (95% over 22bp)
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTTG10250.2697730484880866TruSeq Adapter, Index 19 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.1831824797538617No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT6050.15923189691248035TruSeq Adapter, Index 19 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA5790.15238887324351427No Hit
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC5700.15002013428117986TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC4830.12712232431194714TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC4660.12264803960531545TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC4650.12238484638727828TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT4390.1155418227183122TruSeq Adapter, Index 13 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4210.11080434479364336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG351.0171607E-746.0000044
CGGGTCA351.0171607E-746.0000045
CATGAGG351.0171607E-746.0000041
AGGGTAC351.0171607E-746.0000045
TACGGGT351.0171607E-746.0000043
CCATAGG351.0171607E-746.0000041
ACTACGG700.046.0000041
CGGGAGT351.0171607E-746.0000045
GCAATGG351.0171607E-746.0000041
TACAGGT351.0171607E-746.00000427
CTATGTG206.3060934E-446.09
CGGGTAT453.092282E-1046.05
CTGTCGG206.3060934E-446.01
ATCTCGG650.046.01
CTCACGG206.3060934E-446.01
GGTACGG253.4128076E-546.01
AGATCGT206.3060934E-446.036
GATAACT253.4128076E-546.08
CTATCGG206.3060934E-446.01
CCGGCAT206.3060934E-446.042
Produced by FastQC (version 0.11.5)