##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544999_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	379949
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95869972022561	34.0	33.0	34.0	31.0	34.0
2	33.2768239947993	34.0	33.0	34.0	31.0	34.0
3	33.38624394326607	34.0	34.0	34.0	31.0	34.0
4	36.61730653324525	37.0	37.0	37.0	35.0	37.0
5	36.61649063426933	37.0	37.0	37.0	35.0	37.0
6	36.43512681965211	37.0	37.0	37.0	35.0	37.0
7	36.28442501493622	37.0	37.0	37.0	35.0	37.0
8	36.420556443101574	37.0	37.0	37.0	35.0	37.0
9	38.278626868342855	39.0	39.0	39.0	37.0	39.0
10	38.10809082271568	39.0	39.0	39.0	35.0	39.0
11	37.20817530773867	39.0	35.0	39.0	35.0	39.0
12	37.1196607965806	39.0	35.0	39.0	35.0	39.0
13	37.09604973299048	39.0	35.0	39.0	35.0	39.0
14	38.231309991604135	40.0	35.0	41.0	35.0	41.0
15	38.2971661986214	40.0	35.0	41.0	35.0	41.0
16	38.35431597398598	40.0	35.0	41.0	35.0	41.0
17	38.4685023516314	40.0	36.0	41.0	35.0	41.0
18	38.47968806339798	40.0	36.0	41.0	35.0	41.0
19	38.38178018628816	40.0	35.0	41.0	35.0	41.0
20	38.28578309194128	40.0	35.0	41.0	35.0	41.0
21	38.234702552184636	40.0	35.0	41.0	35.0	41.0
22	38.21695280156021	40.0	35.0	41.0	35.0	41.0
23	38.198126590674015	40.0	35.0	41.0	35.0	41.0
24	38.1708808287428	40.0	35.0	41.0	35.0	41.0
25	38.14164795801542	40.0	35.0	41.0	35.0	41.0
26	38.07899481246167	40.0	35.0	41.0	35.0	41.0
27	38.19028869664086	40.0	35.0	41.0	35.0	41.0
28	38.23057568252581	40.0	35.0	41.0	35.0	41.0
29	38.220258508378755	40.0	35.0	41.0	35.0	41.0
30	38.12819878457372	40.0	35.0	41.0	35.0	41.0
31	38.0491960763155	40.0	35.0	41.0	35.0	41.0
32	37.962908179782026	40.0	35.0	41.0	35.0	41.0
33	37.91183290388973	40.0	35.0	41.0	35.0	41.0
34	37.754059097405175	40.0	35.0	41.0	35.0	41.0
35	37.71895438598338	40.0	35.0	41.0	35.0	41.0
36	37.6651155813017	40.0	35.0	41.0	35.0	41.0
37	37.65269022947817	40.0	35.0	41.0	35.0	41.0
38	37.64119658164648	40.0	35.0	41.0	35.0	41.0
39	37.608552726813336	40.0	35.0	41.0	35.0	41.0
40	37.61165314292181	40.0	35.0	41.0	35.0	41.0
41	37.571642509915804	40.0	35.0	41.0	35.0	41.0
42	37.481814664599725	39.0	35.0	41.0	35.0	41.0
43	37.41585318029525	39.0	35.0	41.0	35.0	41.0
44	37.21651853275045	39.0	35.0	41.0	35.0	41.0
45	37.268488665584066	39.0	35.0	41.0	35.0	41.0
46	37.23751871961763	39.0	35.0	41.0	35.0	41.0
47	37.20076115478656	38.0	35.0	41.0	35.0	41.0
48	37.2062934762297	38.0	35.0	41.0	35.0	41.0
49	37.13973717525247	38.0	35.0	41.0	35.0	41.0
50	37.07458632605955	38.0	35.0	41.0	35.0	41.0
51	37.00402422430379	37.0	35.0	41.0	34.0	41.0
52	36.614756190962474	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	4.0
16	11.0
17	10.0
18	27.0
19	33.0
20	67.0
21	130.0
22	211.0
23	343.0
24	640.0
25	1112.0
26	1670.0
27	1880.0
28	1760.0
29	1741.0
30	1832.0
31	2391.0
32	3288.0
33	5403.0
34	18341.0
35	103200.0
36	11018.0
37	17137.0
38	31353.0
39	174823.0
40	1522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.087195913135712	22.26114557480083	24.746479132725707	22.905179379337753
2	25.2712864094918	24.313263095836547	28.025866629468695	22.38958386520296
3	25.571326678054163	25.265232965476947	27.0604739057084	22.102966450760498
4	26.180618977810177	27.968227314718554	24.015591566236523	21.835562141234742
5	23.297337274213117	34.59990682960081	21.09704197142248	21.005713924763587
6	85.89284351320835	2.414534582272884	8.098192125785303	3.5944297787334616
7	85.72782136549905	1.8694614277179307	6.247943802984085	6.154773403798931
8	75.45723241803505	3.784455282156289	12.953580612134786	7.804731687673872
9	54.128054028303794	14.84330791764158	15.347849316618808	15.680788737435813
10	47.26213254936847	16.590121305754195	24.915185985487525	11.232560159389813
11	44.017486557406386	14.799617843447411	23.70002289780997	17.482872701336234
12	19.27390255007909	36.5214805144901	28.0974551847748	16.10716175065601
13	11.802373476440259	41.17763173478546	28.039815870024658	18.980178918749623
14	10.42087227496322	21.2820668037026	51.381632798086066	16.91542812324812
15	11.911598661925678	15.637098663241645	52.15410489302512	20.297197781807558
16	12.46430441980371	19.563941476356035	29.16707242287781	38.804681680962446
17	16.29666086764276	22.176923745028937	37.80981131678199	23.71660407054631
18	24.051122650671537	26.865447731142865	28.376439995894188	20.706989622291413
19	26.839128409339146	21.912414560901595	25.56369407473108	25.684762955028173
20	16.824100076589225	33.51318203232539	27.097584149451638	22.565133741633744
21	18.50537835341059	23.963479309065164	24.6083026932562	32.922839644268045
22	15.544191457274529	33.216826468815555	21.54420724886761	29.69477482504231
23	15.872393400166866	22.180608450081458	39.23526578567124	22.711732364080444
24	16.518532750448088	21.93425959799868	36.91732311441799	24.629884537135247
25	13.851069485641492	31.572132049301356	30.924676732929946	23.652121732127206
26	17.01465196644813	30.72254434147741	27.045998278716354	25.216805413358106
27	17.826076657656685	26.809124382482914	38.95443862202559	16.410360337834813
28	16.606439285272497	24.242200926966515	34.06588779020342	25.08547199755757
29	18.33772427352092	30.71149022631985	28.0513963716183	22.899389128540935
30	27.898481111938704	23.4636753880126	23.96321611584713	24.674627384201564
31	32.17326535929822	25.417358645502425	21.79529357887506	20.614082416324297
32	23.737396334771248	26.453287151696674	27.66845023937423	22.140866274157847
33	21.80161021610795	25.208120037162885	29.163124524607248	23.827145222121917
34	19.095457548249897	20.951232928629896	34.824147451368475	25.129162071751736
35	33.06443759557204	17.877925721610005	30.855193723368135	18.202442959449822
36	23.142316468789232	23.859781181158525	31.532916259813813	21.464986090238426
37	22.070593684941926	25.72687386991412	29.12890940626242	23.073623038881532
38	17.925037307638654	24.778325512108204	31.244982879281167	26.051654300971972
39	24.824384325264706	19.7655474813725	32.844408065292974	22.56566012806982
40	15.30521201529679	17.580517385228017	34.746242258829476	32.368028340645715
41	21.574474468941883	22.99440188025235	26.47592176844787	28.955201882357894
42	21.114939110249008	19.661322967029786	31.349996973277992	27.873740949443217
43	24.480127596072105	25.467891743365556	29.84111025427097	20.21087040629137
44	15.96819573153239	30.692277121403134	28.784126290633743	24.55540085643073
45	23.049672456040152	27.433945082103122	27.421838194073416	22.09454426778331
46	23.467886479501196	20.508805129109433	29.565283761768026	26.458024629621345
47	22.79069032949159	16.548536777304324	34.692287649131856	25.96848524407223
48	21.113096757722747	14.923318655924875	32.03798404522713	31.925600541125256
49	20.56933956925798	22.63619591050378	32.2524865179274	24.541978002310834
50	19.913725263127418	25.10021081776765	28.549621133362635	26.436442785742297
51	18.701457300848272	18.89964179403025	34.06378224445913	28.33511866066235
52	22.446959986735063	17.281793082755843	36.04957507454948	24.221671855959613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	97.0
2	126.0
3	680.5
4	1235.0
5	894.5
6	554.0
7	602.0
8	650.0
9	704.0
10	758.0
11	738.5
12	719.0
13	710.0
14	625.5
15	550.0
16	499.5
17	449.0
18	433.5
19	418.0
20	407.5
21	397.0
22	454.0
23	511.0
24	605.5
25	700.0
26	754.0
27	808.0
28	1049.5
29	1291.0
30	1459.0
31	1627.0
32	1896.5
33	2166.0
34	2559.5
35	2953.0
36	3774.5
37	4596.0
38	5563.5
39	8134.5
40	9738.0
41	16333.5
42	22929.0
43	34448.5
44	45968.0
45	54659.0
46	63350.0
47	61028.5
48	58707.0
49	49607.5
50	40508.0
51	34105.5
52	27703.0
53	23884.5
54	20066.0
55	16611.5
56	13157.0
57	12106.0
58	11055.0
59	10653.0
60	10251.0
61	9707.0
62	9163.0
63	8169.0
64	5975.0
65	4775.0
66	3714.5
67	2654.0
68	2052.5
69	1451.0
70	1362.0
71	1273.0
72	1083.0
73	893.0
74	683.5
75	474.0
76	504.5
77	535.0
78	376.5
79	218.0
80	127.5
81	37.0
82	29.0
83	21.0
84	20.0
85	19.0
86	20.0
87	21.0
88	10.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	379949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.708066550678943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.29967786972706	18.508604084852834
2	12.301867964751736	6.5711821660050935
3	4.451928170073023	3.5670718153545793
4	2.1620213045057244	2.3097363553889836
5	1.278404806659974	1.7071860327491215
6	0.8366098385548683	1.340653874704571
7	0.6157128266870998	1.1511149405885999
8	0.4737790243529317	1.0122977370187072
9	0.4078097930417804	0.980262998332063
>10	7.592458793896989	48.07293034085531
>50	0.48677296374772233	8.56475429605858
>100	0.08496037354939712	3.8353590677765053
>500	0.003998135225853982	0.6540425778556718
>1k	0.003998135225853982	1.724803712459386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2229	0.5866576830048242	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	1747	0.45979855191091434	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	1460	0.3842620983342501	TruSeq Adapter, Index 19 (95% over 22bp)
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTTG	1025	0.2697730484880866	TruSeq Adapter, Index 19 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.1831824797538617	No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	605	0.15923189691248035	TruSeq Adapter, Index 19 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	579	0.15238887324351427	No Hit
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	570	0.15002013428117986	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	483	0.12712232431194714	TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	466	0.12264803960531545	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	465	0.12238484638727828	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	439	0.1155418227183122	TruSeq Adapter, Index 13 (95% over 22bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	421	0.11080434479364336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.469273507760252	0.0
2	0.0	0.0	0.0	1.7362856593911289	0.0
3	0.0	0.0	0.0	2.721944260940284	0.0
4	0.0	0.0	0.0	3.3272886624257465	0.0
5	0.0	0.0	0.0	6.5050835770063875	0.0
6	0.0	0.0	0.0	7.216494845360825	0.0
7	0.0	0.0	0.0	10.858299403340975	0.0
8	0.0	0.0	0.0	14.54405722873333	0.0
9	0.0	0.0	0.0	18.57933564767903	0.0
10	0.0	0.0	0.0	19.908724591984715	0.0
11	0.0	0.0	0.0	21.22495387538854	0.0
12	0.0	0.0	0.0	22.40353310575893	0.0
13	0.0	0.0	0.0	22.796743773506446	0.0
14	0.0	0.0	0.0	23.101258326775437	0.0
15	0.0	0.0	0.0	24.142713890548468	0.0
16	0.0	0.0	0.0	25.193907603388876	0.0
17	0.0	0.0	0.0	26.924666205201223	0.0
18	0.0	0.0	0.0	27.527115481288277	0.0
19	0.0	0.0	0.0	28.35301579948888	0.0
20	0.0	0.0	0.0	29.257084503446514	0.0
21	0.0	0.0	0.0	29.97349644294366	0.0
22	0.0	0.0	0.0	30.86782699783392	0.0
23	0.0	0.0	0.0	31.469223501048823	0.0
24	0.0	0.0	0.0	31.924021381817035	0.0
25	0.0	0.0	0.0	32.424614882523706	0.0
26	0.0	0.0	0.0	32.86362117020968	0.0
27	0.0	0.0	0.0	33.30210107145959	0.0
28	0.0	0.0	0.0	33.69504854598907	0.0
29	0.0	0.0	0.0	34.32723865571432	0.0
30	0.0	0.0	0.0	34.930477511455486	0.0
31	0.0	0.0	0.0	35.38027472108099	0.0
32	0.0	0.0	0.0	35.826650418872006	0.0
33	0.0	0.0	0.0	36.27934275389592	0.0
34	0.0	0.0	0.0	36.901005134899684	0.0
35	0.0	0.0	0.0	37.37054183587797	0.0
36	0.0	0.0	0.0	37.84929029948756	0.0
37	0.0	0.0	0.0	38.24013222827274	0.0
38	0.0	0.0	0.0	38.62465751982503	0.0
39	0.0	0.0	0.0	39.092878254713135	0.0
40	0.0	0.0	0.0	39.52819983734659	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	35	1.0171607E-7	46.000004	4
CGGGTCA	35	1.0171607E-7	46.000004	5
CATGAGG	35	1.0171607E-7	46.000004	1
AGGGTAC	35	1.0171607E-7	46.000004	5
TACGGGT	35	1.0171607E-7	46.000004	3
CCATAGG	35	1.0171607E-7	46.000004	1
ACTACGG	70	0.0	46.000004	1
CGGGAGT	35	1.0171607E-7	46.000004	5
GCAATGG	35	1.0171607E-7	46.000004	1
TACAGGT	35	1.0171607E-7	46.000004	27
CTATGTG	20	6.3060934E-4	46.0	9
CGGGTAT	45	3.092282E-10	46.0	5
CTGTCGG	20	6.3060934E-4	46.0	1
ATCTCGG	65	0.0	46.0	1
CTCACGG	20	6.3060934E-4	46.0	1
GGTACGG	25	3.4128076E-5	46.0	1
AGATCGT	20	6.3060934E-4	46.0	36
GATAACT	25	3.4128076E-5	46.0	8
CTATCGG	20	6.3060934E-4	46.0	1
CCGGCAT	20	6.3060934E-4	46.0	42
>>END_MODULE