FastQCFastQC Report
Thu 26 May 2016
SRR1544998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544998_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences951851
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT34940.3670742584711263RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT28490.299311551913062TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT23750.24951384197736828RNA PCR Primer, Index 35 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.20927645188164953No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11800.12396898254033457No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11550.12134252104583594No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11200.1176654749535379No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT253.4167104E-546.029
ATTACGG650.046.01
ATATCGA253.4167104E-546.032
TCGAATC206.310905E-446.029
TACACGG600.046.01
TACGGGT1350.044.29633
CGTAGGG3500.042.0571442
CGGGTGC1650.041.8181845
AGTACGG554.7293724E-1141.818181
ACGGGTG2100.041.6190454
ATTGCGG2100.041.6190451
TAAACGG1000.041.3999981
CTACCGG1000.041.3999981
TATACGG508.712959E-1041.3999981
ATACCGG451.5879777E-840.888891
TTGCGGG7150.040.8531462
ACGGGTA1300.040.692314
CGTTTTT14900.040.5973171
TCGAAGG1700.040.5882341
TAGCGGG7350.040.3673482
Produced by FastQC (version 0.11.5)