##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544998_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	951851
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.888500406050944	34.0	33.0	34.0	31.0	34.0
2	33.22117957537472	34.0	33.0	34.0	31.0	34.0
3	33.28953165989215	34.0	34.0	34.0	31.0	34.0
4	36.57133101714449	37.0	37.0	37.0	35.0	37.0
5	36.5692256456105	37.0	37.0	37.0	35.0	37.0
6	36.6564042061205	37.0	37.0	37.0	35.0	37.0
7	36.482402182694564	37.0	37.0	37.0	35.0	37.0
8	36.50050165414545	37.0	37.0	37.0	35.0	37.0
9	38.261815137033004	39.0	39.0	39.0	37.0	39.0
10	38.15331286094147	39.0	39.0	39.0	35.0	39.0
11	37.751248882440635	39.0	37.0	39.0	35.0	39.0
12	37.7068648349374	39.0	37.0	39.0	35.0	39.0
13	37.6924487130864	39.0	37.0	39.0	35.0	39.0
14	39.09174124941824	41.0	38.0	41.0	35.0	41.0
15	39.14299612019108	41.0	38.0	41.0	35.0	41.0
16	39.141409737448406	41.0	38.0	41.0	35.0	41.0
17	39.16407820131512	41.0	38.0	41.0	35.0	41.0
18	39.14429989567695	41.0	38.0	41.0	35.0	41.0
19	39.09010023627648	41.0	38.0	41.0	35.0	41.0
20	39.018892662822225	41.0	38.0	41.0	35.0	41.0
21	38.97034094621952	41.0	38.0	41.0	35.0	41.0
22	38.9305847238696	41.0	37.0	41.0	35.0	41.0
23	38.91494572154676	41.0	37.0	41.0	35.0	41.0
24	38.85564442333937	41.0	37.0	41.0	35.0	41.0
25	38.82335050338761	41.0	37.0	41.0	35.0	41.0
26	38.75873744945375	41.0	37.0	41.0	35.0	41.0
27	38.70724409597721	41.0	36.0	41.0	35.0	41.0
28	38.69714062390017	41.0	36.0	41.0	35.0	41.0
29	38.710842348224666	41.0	36.0	41.0	35.0	41.0
30	38.66341580772621	41.0	36.0	41.0	35.0	41.0
31	38.55903392442725	40.0	36.0	41.0	35.0	41.0
32	38.51253084779025	40.0	36.0	41.0	35.0	41.0
33	38.40984986095513	40.0	36.0	41.0	35.0	41.0
34	38.29683427343145	40.0	36.0	41.0	35.0	41.0
35	38.202146134216385	40.0	36.0	41.0	35.0	41.0
36	38.199039555560695	40.0	35.0	41.0	35.0	41.0
37	38.153415818232055	40.0	35.0	41.0	35.0	41.0
38	38.056098065768694	40.0	35.0	41.0	35.0	41.0
39	37.99294742559497	40.0	35.0	41.0	35.0	41.0
40	37.91727591818467	40.0	35.0	41.0	35.0	41.0
41	37.84577418104304	40.0	35.0	41.0	35.0	41.0
42	37.76399667595033	40.0	35.0	41.0	35.0	41.0
43	37.652956187470515	40.0	35.0	41.0	34.0	41.0
44	37.586008734560345	40.0	35.0	41.0	34.0	41.0
45	37.523643931665774	40.0	35.0	41.0	34.0	41.0
46	37.49185429232096	39.0	35.0	41.0	34.0	41.0
47	37.39180817165712	39.0	35.0	41.0	34.0	41.0
48	37.36913025252902	39.0	35.0	41.0	34.0	41.0
49	37.30033482131132	39.0	35.0	41.0	34.0	41.0
50	37.22697985293917	39.0	35.0	41.0	34.0	41.0
51	37.1285222161872	39.0	35.0	41.0	34.0	41.0
52	36.90509544035779	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	7.0
14	10.0
15	14.0
16	29.0
17	60.0
18	103.0
19	132.0
20	302.0
21	465.0
22	701.0
23	1059.0
24	1722.0
25	2829.0
26	3980.0
27	4809.0
28	5084.0
29	5514.0
30	6398.0
31	7985.0
32	10086.0
33	13935.0
34	40141.0
35	133931.0
36	36481.0
37	59746.0
38	106616.0
39	505787.0
40	3921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.753287016560364	22.693362721686483	23.32602476648131	20.227325495271845
2	27.635417728194856	24.053974834296543	25.939669128886766	22.37093830862183
3	27.77283419358702	24.506986912867664	24.829306267472536	22.890872626072778
4	26.520012060711185	27.419207417967726	21.946292014191297	24.114488507129792
5	24.70145012192034	36.26313362070324	19.78345350270158	19.25196275467484
6	87.71719523328755	3.027364577018882	6.685290029636992	2.5701501600565635
7	87.03820240772978	2.8686212442913863	5.554335710105889	4.538840637872944
8	76.18408763556481	5.676938932669084	11.187360206586956	6.951613225179151
9	44.33981789166582	20.531049502495662	18.544604144976475	16.584528460862046
10	34.82887552778744	23.05549923254795	25.398828177939613	16.716797061724996
11	34.31188284721033	18.229533824096418	26.404132579573904	21.054450749119347
12	22.87353798020909	27.129666302814197	29.344088518055873	20.65270719892084
13	17.26436175409807	30.130556147968534	29.487703432575056	23.117378665358338
14	15.10183841798769	25.72913197548776	38.4613768331388	20.70765277338575
15	17.75477464435085	20.1950725481194	40.877301174238404	21.172851633291344
16	18.98322321455774	22.498689395714244	28.873531676701504	29.644555713026516
17	19.891453599355362	23.22884569118486	31.214234160598664	25.665466548861115
18	23.36605203965747	27.485604364548657	27.67502476753189	21.473318828261988
19	26.069836560554123	22.782557354039657	26.598280613247244	24.549325472158984
20	22.764592357417285	28.261986382322448	26.766164031975592	22.20725722828468
21	21.08659863781201	23.911935796674058	26.198848349163896	28.80261721635004
22	20.017313634171735	28.013838300322213	25.40702273780245	26.561825327703602
23	19.83860919408605	22.488183549736252	33.320025928427874	24.353181327749827
24	21.22485557088242	22.917137241017766	30.46579769312634	25.39220949497348
25	19.68459349204865	27.61325039318129	28.367885309780625	24.334270804989437
26	21.129672606321787	27.043203190415305	26.96325370252277	24.863870500740138
27	22.786654633971075	25.751929661260007	31.60347575408336	19.85793995068556
28	20.127099724641777	25.245547885120672	29.08343847934183	25.543913910895714
29	23.4926474836923	25.278116007652457	27.64634380801197	23.582892700643274
30	26.898537691298323	23.770106875971134	24.74641514270616	24.584940290024385
31	27.8164334543957	24.21261310856426	26.416424419368155	21.554529017671882
32	27.01641328317142	24.176367939940178	26.454665698728057	22.35255307816034
33	23.356071485978372	22.8043044552141	29.529831874946815	24.309792183860708
34	24.682434540700175	21.25962992106958	30.29171582527097	23.766219712959277
35	25.139123665363595	21.965097478491906	31.70391164163299	21.191867214511515
36	24.275648184432228	24.825208987541117	28.76479617082926	22.134346657197398
37	23.425620186352695	24.733808127532566	30.76973181726972	21.070839868845017
38	22.21986424345827	23.50704049268215	28.46054687130654	25.812548392553037
39	25.48812786875257	21.07020951808634	30.46149029627536	22.98017231688573
40	19.977076244076017	20.27344615911524	31.608098326313677	28.141379270495065
41	23.13198179126775	24.02245729636256	26.044307354827595	26.801253557542093
42	23.352289381426296	21.176213504004306	29.298072912672257	26.173424201897145
43	23.406814722052086	24.984057378728394	29.44725592555978	22.161871973659743
44	21.331174732179722	25.175158717068115	29.30689782329377	24.186768727458393
45	23.071783293813844	24.605531748141253	27.90720396364557	24.41548099439933
46	24.82237240912706	21.65528008060085	29.472995248205862	24.049352262066225
47	22.746837477714475	19.90983882981685	32.60804474649919	24.735278945969487
48	21.496221572494015	20.195387723498744	29.506613955335446	28.8017767486718
49	21.285054068336326	22.68422263568563	31.912032450457055	24.11869084552099
50	21.14711231064526	23.185876781134862	29.708641373492277	25.9583695347276
51	21.35113583953791	20.89738835174833	31.01399273625809	26.73748307245567
52	22.6550163838668	19.469223649499764	32.95137579306005	24.924384173573387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	348.0
2	525.0
3	2157.0
4	3789.0
5	2576.0
6	1363.0
7	1389.0
8	1415.0
9	1394.5
10	1374.0
11	1319.5
12	1265.0
13	1272.0
14	1241.5
15	1204.0
16	1182.5
17	1161.0
18	1150.0
19	1139.0
20	1265.5
21	1392.0
22	1741.0
23	2090.0
24	2395.0
25	2700.0
26	3567.0
27	4434.0
28	6282.0
29	8130.0
30	8541.0
31	8952.0
32	10663.0
33	12374.0
34	13383.0
35	14392.0
36	16682.5
37	18973.0
38	21362.5
39	26890.0
40	30028.0
41	36829.5
42	43631.0
43	53805.5
44	63980.0
45	73361.5
46	82743.0
47	91253.5
48	99764.0
49	103111.5
50	106459.0
51	95815.5
52	85172.0
53	75339.5
54	65507.0
55	56195.5
56	46884.0
57	43784.5
58	40685.0
59	39877.0
60	39069.0
61	38840.0
62	38611.0
63	36537.5
64	27987.0
65	21510.0
66	17769.0
67	14028.0
68	11444.0
69	8860.0
70	7551.5
71	6243.0
72	5546.5
73	4850.0
74	4097.0
75	3344.0
76	2907.0
77	2470.0
78	1697.5
79	925.0
80	645.5
81	366.0
82	314.0
83	262.0
84	171.0
85	80.0
86	56.0
87	32.0
88	18.5
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	951851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.926295077286802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92463631824558	17.678884131178414
2	11.489449739568117	5.727788289682618
3	3.982444651425679	2.978027715311972
4	1.8754775431758728	1.869948266081068
5	1.1132784064882704	1.3874953031649135
6	0.7293854045046374	1.0908525490649272
7	0.5245667466220735	0.9152853859823901
8	0.46246199676642913	0.9221971354745016
9	0.37193953403689817	0.8343967118681054
>10	7.8014461966209465	49.93317396672344
>50	0.6126088728990269	9.56657753262581
>100	0.10217184471594326	4.3263442875905485
>500	0.007177360992442294	1.2783289725459108
>1k	0.002955383938064474	1.4906997527054273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3494	0.3670742584711263	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT	2849	0.299311551913062	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	2375	0.24951384197736828	RNA PCR Primer, Index 35 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1992	0.20927645188164953	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1180	0.12396898254033457	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1155	0.12134252104583594	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1120	0.1176654749535379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3081364625345774	0.0
2	1.0505845977994455E-4	0.0	0.0	1.1105729783337939	0.0
3	1.0505845977994455E-4	0.0	0.0	1.750694173772996	0.0
4	1.0505845977994455E-4	0.0	0.0	2.148445502499866	0.0
5	1.0505845977994455E-4	0.0	0.0	4.3338715828422725	0.0
6	1.0505845977994455E-4	0.0	0.0	4.748327206674154	0.0
7	1.0505845977994455E-4	0.0	0.0	6.749375690102758	0.0
8	1.0505845977994455E-4	0.0	0.0	8.84959935956363	0.0
9	1.0505845977994455E-4	0.0	0.0	10.925239349435994	0.0
10	1.0505845977994455E-4	0.0	0.0	11.866037856765397	0.0
11	1.0505845977994455E-4	0.0	0.0	12.7691203770338	0.0
12	1.0505845977994455E-4	0.0	0.0	13.543611342531552	0.0
13	1.0505845977994455E-4	0.0	0.0	13.845549355939113	0.0
14	1.0505845977994455E-4	0.0	0.0	14.084137118099367	0.0
15	1.0505845977994455E-4	0.0	0.0	14.889410212312642	0.0
16	1.0505845977994455E-4	0.0	0.0	15.749103588691927	0.0
17	1.0505845977994455E-4	0.0	0.0	16.92103070753721	0.0
18	1.0505845977994455E-4	0.0	0.0	17.443486428022872	0.0
19	1.0505845977994455E-4	0.0	0.0	18.102623204682246	0.0
20	1.0505845977994455E-4	0.0	0.0	18.842339819992834	0.0
21	1.0505845977994455E-4	0.0	0.0	19.510091390354162	0.0
22	1.0505845977994455E-4	0.0	0.0	20.227535612191403	0.0
23	1.0505845977994455E-4	0.0	0.0	20.76427928320714	0.0
24	1.0505845977994455E-4	0.0	0.0	21.235886709159313	0.0
25	1.0505845977994455E-4	0.0	0.0	21.67660694793618	0.0
26	1.0505845977994455E-4	0.0	0.0	22.094424442481017	0.0
27	1.0505845977994455E-4	0.0	0.0	22.548592164109717	0.0
28	1.0505845977994455E-4	0.0	0.0	22.98489994757583	0.0
29	1.0505845977994455E-4	0.0	0.0	23.520487975533985	0.0
30	1.0505845977994455E-4	0.0	0.0	24.037060422271974	0.0
31	1.0505845977994455E-4	0.0	0.0	24.500683405280867	0.0
32	1.0505845977994455E-4	0.0	0.0	24.95422077615089	0.0
33	1.0505845977994455E-4	0.0	0.0	25.367100523086073	0.0
34	1.0505845977994455E-4	0.0	0.0	25.8818869760078	0.0
35	1.0505845977994455E-4	0.0	0.0	26.315253122600073	0.0
36	1.0505845977994455E-4	0.0	0.0	26.781397508643686	0.0
37	1.0505845977994455E-4	0.0	0.0	27.212662486040358	0.0
38	1.0505845977994455E-4	0.0	0.0	27.61881849154962	0.0
39	1.0505845977994455E-4	0.0	0.0	28.075822791592383	0.0
40	1.0505845977994455E-4	0.0	0.0	28.538815423842596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	25	3.4167104E-5	46.0	29
ATTACGG	65	0.0	46.0	1
ATATCGA	25	3.4167104E-5	46.0	32
TCGAATC	20	6.310905E-4	46.0	29
TACACGG	60	0.0	46.0	1
TACGGGT	135	0.0	44.2963	3
CGTAGGG	350	0.0	42.057144	2
CGGGTGC	165	0.0	41.818184	5
AGTACGG	55	4.7293724E-11	41.81818	1
ACGGGTG	210	0.0	41.619045	4
ATTGCGG	210	0.0	41.619045	1
TAAACGG	100	0.0	41.399998	1
CTACCGG	100	0.0	41.399998	1
TATACGG	50	8.712959E-10	41.399998	1
ATACCGG	45	1.5879777E-8	40.88889	1
TTGCGGG	715	0.0	40.853146	2
ACGGGTA	130	0.0	40.69231	4
CGTTTTT	1490	0.0	40.597317	1
TCGAAGG	170	0.0	40.588234	1
TAGCGGG	735	0.0	40.367348	2
>>END_MODULE