FastQCFastQC Report
Thu 26 May 2016
SRR1544997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544997_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences536910
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT24120.4492373023411745RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT20160.3754819243448623TruSeq Adapter, Index 10 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA16480.306941573075562No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT16200.30172654634855RNA PCR Primer, Index 35 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.2238736473524427No Hit
TGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTTG11470.21362984485295486RNA PCR Primer, Index 35 (96% over 27bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6460.12031811663034772No Hit
ACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC6050.11268182749436591RNA PCR Primer, Index 35 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC5600.1043005345402395RNA PCR Primer, Index 35 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC5500.1024380249948781No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT5490.10225177404034194RNA PCR Primer, Index 35 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC5460.10169302117673353TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTC206.3084334E-446.00000411
CAATTGG206.3084334E-446.0000041
TACACGG405.6024874E-946.0000041
TGCGTGA206.3084334E-446.00000414
TATCCGG405.6024874E-946.0000041
GTACCGA301.8595365E-646.07
GTCGAGG501.6370905E-1146.01
CATGCGG1150.046.01
TCCGAAT253.4147066E-546.039
ACGGGTA650.046.04
CCGTAGG253.4147066E-546.01
CACTAGG501.6370905E-1146.01
GCGATGA600.046.08
ATTACGG453.092282E-1045.9999961
CGGTATT453.092282E-1045.99999643
ATTGCGG1450.044.4137951
ACGGGAT1250.044.164
TTGCGGG4800.043.6041682
CGTAGGG2200.042.8636362
TACGGGT700.042.7142873
Produced by FastQC (version 0.11.5)