##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544997_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	536910
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87678568102662	34.0	33.0	34.0	31.0	34.0
2	33.25401091430594	34.0	33.0	34.0	31.0	34.0
3	33.38737218528245	34.0	34.0	34.0	31.0	34.0
4	36.61265947737982	37.0	37.0	37.0	35.0	37.0
5	36.62112644577304	37.0	37.0	37.0	35.0	37.0
6	36.41918571082677	37.0	37.0	37.0	35.0	37.0
7	36.231962526307946	37.0	37.0	37.0	35.0	37.0
8	36.36124490138012	37.0	37.0	37.0	35.0	37.0
9	38.204607848615225	39.0	39.0	39.0	37.0	39.0
10	38.17084241306737	39.0	39.0	39.0	35.0	39.0
11	37.80330409193347	39.0	37.0	39.0	35.0	39.0
12	37.80830493006277	39.0	38.0	39.0	35.0	39.0
13	37.79246242386992	39.0	38.0	39.0	35.0	39.0
14	39.12438397496787	41.0	38.0	41.0	35.0	41.0
15	39.14985379300069	41.0	38.0	41.0	35.0	41.0
16	39.17920135590695	41.0	38.0	41.0	35.0	41.0
17	39.22871430966084	41.0	38.0	41.0	35.0	41.0
18	39.22555921849099	41.0	38.0	41.0	35.0	41.0
19	39.20375109422436	41.0	38.0	41.0	35.0	41.0
20	39.1388091113967	41.0	38.0	41.0	35.0	41.0
21	39.08844499078058	41.0	38.0	41.0	35.0	41.0
22	39.05602428712447	41.0	38.0	41.0	35.0	41.0
23	39.00930137266953	41.0	38.0	41.0	35.0	41.0
24	38.96992792088059	41.0	38.0	41.0	35.0	41.0
25	38.93009442923395	41.0	37.0	41.0	35.0	41.0
26	38.843199046395114	41.0	37.0	41.0	35.0	41.0
27	38.886535918496584	41.0	37.0	41.0	35.0	41.0
28	38.85708778007487	41.0	37.0	41.0	35.0	41.0
29	38.87940250693785	41.0	37.0	41.0	35.0	41.0
30	38.847603881469894	41.0	37.0	41.0	35.0	41.0
31	38.726622711441394	41.0	37.0	41.0	35.0	41.0
32	38.69930714644912	41.0	37.0	41.0	35.0	41.0
33	38.58026112383826	41.0	36.0	41.0	35.0	41.0
34	38.444478590452775	40.0	36.0	41.0	35.0	41.0
35	38.350462833622025	40.0	36.0	41.0	35.0	41.0
36	38.374271293140374	40.0	36.0	41.0	35.0	41.0
37	38.33997504237209	40.0	36.0	41.0	35.0	41.0
38	38.26727011975937	40.0	36.0	41.0	35.0	41.0
39	38.228930360768096	40.0	35.0	41.0	35.0	41.0
40	38.17560484997485	40.0	35.0	41.0	35.0	41.0
41	38.12826730736995	40.0	35.0	41.0	35.0	41.0
42	38.04395895028962	40.0	35.0	41.0	35.0	41.0
43	37.93988005438528	40.0	35.0	41.0	35.0	41.0
44	37.806636121510124	40.0	35.0	41.0	35.0	41.0
45	37.79641466912518	40.0	35.0	41.0	35.0	41.0
46	37.72097371999031	40.0	35.0	41.0	35.0	41.0
47	37.61237451341938	40.0	35.0	41.0	35.0	41.0
48	37.5369615019277	39.0	35.0	41.0	35.0	41.0
49	37.46485444487903	39.0	35.0	41.0	35.0	41.0
50	37.38979717271051	39.0	35.0	41.0	35.0	41.0
51	37.29504758711889	39.0	35.0	41.0	34.0	41.0
52	36.833486059116055	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	0.0
13	3.0
14	5.0
15	8.0
16	8.0
17	29.0
18	44.0
19	78.0
20	138.0
21	239.0
22	412.0
23	552.0
24	911.0
25	1395.0
26	2072.0
27	2446.0
28	2482.0
29	2514.0
30	2816.0
31	3418.0
32	4570.0
33	6595.0
34	21861.0
35	73013.0
36	19501.0
37	33603.0
38	63609.0
39	293329.0
40	1255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.25808794770073	22.197388761617404	22.668044923730235	21.87647836695163
2	27.547447430668083	24.245590508651357	25.938052932521277	22.26890912815928
3	27.981225903782757	24.29960328546684	24.938444059525803	22.7807267512246
4	26.33774748095584	27.038423571920806	22.4756476876944	24.148181259428956
5	24.476541692276175	35.86765007170662	20.11398558417612	19.54182265184109
6	86.38729023486246	3.062896947346855	7.344061388314616	3.205751429476076
7	85.68084036430686	2.7870592836788286	6.309809837775418	5.222290514238885
8	74.2968095211488	5.785885902665251	12.255871561341753	7.661433014844202
9	44.27315564992271	19.970199847274216	18.776331228697547	16.98031327410553
10	34.261794341696	22.522210426328435	26.40535657745246	16.810638654523107
11	33.68534763740664	18.008791045054107	26.510960868674456	21.7949004488648
12	22.37861094038107	26.52790970553724	29.986403680318862	21.10707567376283
13	17.138999087370323	29.47421355534447	29.758805013875694	23.62798234340951
14	14.87791249930156	25.36887001545883	38.25520105790542	21.49801642733419
15	17.474064554580842	19.950084744184313	40.855264383229965	21.72058631800488
16	18.807062636196008	22.97442774394219	29.025162503957837	29.19334711590397
17	19.42169078616528	23.35028217019612	31.490752640107278	25.73727440353132
18	22.734722765454173	27.714514536887002	27.805777504609715	21.744985193049114
19	25.70002421262409	22.712745152818908	26.546907302899925	25.040323331657078
20	22.092156972304483	28.808738894786835	26.785867277569796	22.313236855338882
21	21.00314764113166	23.659645005680655	25.98964444692779	29.347562906259895
22	19.981002402637312	28.204727049226126	25.20515542642156	26.609115121715
23	19.611666759792143	22.47378517814904	33.402804939375315	24.511743122683505
24	21.524464062878323	23.063828202119534	29.91320705518616	25.498500679815983
25	19.992549961818554	27.304017433089346	28.38948799612598	24.31394460896612
26	21.479950084744186	26.30105604291222	26.766683429252573	25.45231044309102
27	23.34115587342385	25.21986925182992	31.57568307537576	19.863291799370472
28	20.607550613696894	24.769328192806988	28.97990352200555	25.643217671490564
29	24.123596133430183	24.721834199400273	27.526028570896425	23.628541096273118
30	26.992047084241307	23.641764914045186	24.500940567320406	24.8652474343931
31	28.199512022499118	23.42478255201058	26.25523830809633	22.12046711739398
32	27.132666554916096	23.684602633588497	26.669646681939245	22.513084129556162
33	23.61252351418301	22.176901156618428	29.74036616937662	24.47020915982194
34	25.42213778845617	20.597865564061017	30.560056620290176	23.41994002719264
35	24.977743010932933	21.421281034065302	32.541021772736585	21.059954182265184
36	24.75126185021698	24.16922761729154	28.725670969063717	22.353839563427762
37	23.691307667951797	24.184872697472574	31.28811160157196	20.83570803300367
38	22.367063381199827	22.875155985174427	28.33268145499246	26.42509917863329
39	25.800227226164534	20.500828816747685	30.601776834106275	23.097167122981503
40	19.962749809092774	19.737199903149506	31.453688700154586	28.846361587603138
41	23.390326125421392	23.79169693244678	26.088916205695554	26.72906073643627
42	23.169246242386993	20.767353932688906	29.436404611573636	26.62699521335047
43	23.83025088003576	24.666331414948502	29.20377716935799	22.299640535657744
44	21.632489616509286	24.522359427092063	29.580562850384606	24.264588106014042
45	23.026019258348697	24.077219645750684	28.068018848596598	24.828742247304017
46	24.97439049375128	20.89456333463709	29.61036300311039	24.52068316850124
47	22.624648451323313	19.506248719524688	32.84181706431246	25.027285764839547
48	21.496898921606974	19.57367156506677	29.247359892719448	29.682069620606804
49	21.1229070048984	21.937568680039483	32.17578365089121	24.763740664170903
50	21.328341807751762	22.535247993145965	29.958279786183905	26.178130412918364
51	21.439347376655306	20.343074258255573	31.131288297852528	27.086290067236597
52	22.521279171555754	19.32037026689762	33.281183066063214	24.877167495483416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107.0
1	213.0
2	319.0
3	1338.0
4	2357.0
5	1609.0
6	861.0
7	870.5
8	880.0
9	842.5
10	805.0
11	801.5
12	798.0
13	782.0
14	764.5
15	763.0
16	703.5
17	644.0
18	660.0
19	676.0
20	754.0
21	832.0
22	979.5
23	1127.0
24	1291.5
25	1456.0
26	1875.0
27	2294.0
28	3110.5
29	3927.0
30	4240.0
31	4553.0
32	5393.0
33	6233.0
34	6895.5
35	7558.0
36	8582.5
37	9607.0
38	11101.0
39	14145.0
40	15695.0
41	19323.5
42	22952.0
43	28613.5
44	34275.0
45	40093.5
46	45912.0
47	52221.5
48	58531.0
49	60646.0
50	62761.0
51	56267.0
52	49773.0
53	43606.5
54	37440.0
55	32343.0
56	27246.0
57	25023.0
58	22800.0
59	22371.5
60	21943.0
61	21872.5
62	21802.0
63	21115.5
64	16431.5
65	12434.0
66	10225.5
67	8017.0
68	6610.0
69	5203.0
70	4377.5
71	3552.0
72	3183.5
73	2815.0
74	2386.0
75	1957.0
76	1652.0
77	1347.0
78	924.0
79	501.0
80	346.0
81	191.0
82	158.5
83	126.0
84	80.5
85	35.0
86	24.0
87	13.0
88	7.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	536910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.21945538863509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99907441343761	21.757728172598963
2	9.660424877496771	5.838655572415487
3	3.2239072733898277	2.9227416607590024
4	1.6313539530992036	1.9719451203501956
5	1.1592483866236263	1.7515927451959943
6	0.9497153377795381	1.7219928169158756
7	0.8395666204134866	1.7759872221960744
8	0.7348597531387795	1.7765649221504594
9	0.680323821039142	1.8503113823746304
>10	8.898214157169763	49.65007257817403
>50	0.1602151087555962	3.182753227191052
>100	0.055673203814685934	3.275846796270175
>500	0.003711546920979062	0.6460469372237917
>1k	0.003711546920979062	1.8777608461843134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	2412	0.4492373023411745	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT	2016	0.3754819243448623	TruSeq Adapter, Index 10 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1648	0.306941573075562	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	1620	0.30172654634855	RNA PCR Primer, Index 35 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1202	0.2238736473524427	No Hit
TGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTTG	1147	0.21362984485295486	RNA PCR Primer, Index 35 (96% over 27bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	646	0.12031811663034772	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	605	0.11268182749436591	RNA PCR Primer, Index 35 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	560	0.1043005345402395	RNA PCR Primer, Index 35 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	550	0.1024380249948781	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	549	0.10225177404034194	RNA PCR Primer, Index 35 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	546	0.10169302117673353	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38311821348084407	0.0
2	0.0	0.0	0.0	1.3385856102512526	0.0
3	0.0	0.0	0.0	2.0694343558510737	0.0
4	0.0	0.0	0.0	2.5307779702370974	0.0
5	0.0	0.0	0.0	4.984261794341696	0.0
6	0.0	0.0	0.0	5.454545454545454	0.0
7	0.0	0.0	0.0	7.761822279339182	0.0
8	0.0	0.0	0.0	10.072079119405487	0.0
9	0.0	0.0	0.0	12.435417481514593	0.0
10	0.0	0.0	0.0	13.500214188597717	0.0
11	0.0	0.0	0.0	14.496656795366077	0.0
12	0.0	0.0	0.0	15.326777299733662	0.0
13	0.0	0.0	0.0	15.638933899536235	0.0
14	0.0	0.0	0.0	15.896705220614257	0.0
15	0.0	0.0	0.0	16.768173436888866	0.0
16	0.0	0.0	0.0	17.63591663407275	0.0
17	0.0	0.0	0.0	18.809111396695908	0.0
18	0.0	0.0	0.0	19.319066510215865	0.0
19	0.0	0.0	0.0	19.98007114786463	0.0
20	0.0	0.0	0.0	20.735505019463226	0.0
21	0.0	0.0	0.0	21.41736976402004	0.0
22	0.0	0.0	0.0	22.1705686241642	0.0
23	0.0	0.0	0.0	22.70697137322829	0.0
24	0.0	0.0	0.0	23.178745041068336	0.0
25	0.0	0.0	0.0	23.63431487586374	0.0
26	0.0	0.0	0.0	24.056732040751708	0.0
27	1.86250954536142E-4	0.0	0.0	24.48920675718463	0.0
28	1.86250954536142E-4	0.0	0.0	24.92186772457209	0.0
29	1.86250954536142E-4	0.0	0.0	25.429401575683077	0.0
30	1.86250954536142E-4	0.0	0.0	25.916634072749623	0.0
31	1.86250954536142E-4	0.0	0.0	26.386917732953382	0.0
32	1.86250954536142E-4	0.0	0.0	26.831871263340226	0.0
33	1.86250954536142E-4	0.0	0.0	27.251867165819224	0.0
34	1.86250954536142E-4	0.0	0.0	27.75902851502114	0.0
35	1.86250954536142E-4	0.0	0.0	28.175299398409418	0.0
36	1.86250954536142E-4	0.0	0.0	28.619135423069043	0.0
37	1.86250954536142E-4	0.0	0.0	29.026093758730514	0.0
38	1.86250954536142E-4	0.0	0.0	29.44627591216405	0.0
39	1.86250954536142E-4	0.0	0.0	29.90538451509564	0.0
40	1.86250954536142E-4	0.0	0.0	30.361326851800115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTC	20	6.3084334E-4	46.000004	11
CAATTGG	20	6.3084334E-4	46.000004	1
TACACGG	40	5.6024874E-9	46.000004	1
TGCGTGA	20	6.3084334E-4	46.000004	14
TATCCGG	40	5.6024874E-9	46.000004	1
GTACCGA	30	1.8595365E-6	46.0	7
GTCGAGG	50	1.6370905E-11	46.0	1
CATGCGG	115	0.0	46.0	1
TCCGAAT	25	3.4147066E-5	46.0	39
ACGGGTA	65	0.0	46.0	4
CCGTAGG	25	3.4147066E-5	46.0	1
CACTAGG	50	1.6370905E-11	46.0	1
GCGATGA	60	0.0	46.0	8
ATTACGG	45	3.092282E-10	45.999996	1
CGGTATT	45	3.092282E-10	45.999996	43
ATTGCGG	145	0.0	44.413795	1
ACGGGAT	125	0.0	44.16	4
TTGCGGG	480	0.0	43.604168	2
CGTAGGG	220	0.0	42.863636	2
TACGGGT	70	0.0	42.714287	3
>>END_MODULE