FastQCFastQC Report
Thu 26 May 2016
SRR1544996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544996_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences729310
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT29810.40874251004374RNA PCR Primer, Index 34 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT23950.3283925902565439RNA PCR Primer, Index 34 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT23410.3209883314365633TruSeq Adapter, Index 18 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17250.2365249345271558No Hit
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC11590.15891733282143394TruSeq Adapter, Index 3 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT11160.1530213489462643TruSeq Adapter, Index 18 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT7720.10585347794490683TruSeq Adapter, Index 3 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC7650.10489366661639084RNA PCR Primer, Index 34 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC7380.10119153720640056TruSeq Adapter, Index 18 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG551.8189894E-1246.0000041
AACGTCC206.3099293E-446.021
ACGCCTC206.3099293E-446.022
CGACGGT206.3099293E-446.027
TAGGTCG206.3099293E-446.033
CGCATAT206.3099293E-446.028
TACGGGT405.6061253E-946.03
CGCCTAT206.3099293E-446.036
AATGCGG800.046.01
CCGTAGG206.3099293E-446.01
CTCGCTA206.3099293E-446.038
CGTCGGG453.092282E-1046.02
CCGAACA206.3099293E-446.029
CACGTTC206.3099293E-446.046
CCTATCG206.3099293E-446.038
TCGCGTG253.415919E-546.044
ATCGCCG253.415919E-546.021
TATCCGG650.046.01
CTAGCGG1800.046.01
CCCGAAC206.3099293E-446.028
Produced by FastQC (version 0.11.5)