##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544996_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	729310
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9377246986878	34.0	33.0	34.0	31.0	34.0
2	33.24424455992651	34.0	33.0	34.0	31.0	34.0
3	33.293484252238414	34.0	34.0	34.0	31.0	34.0
4	36.58286599662695	37.0	37.0	37.0	35.0	37.0
5	36.57430859305371	37.0	37.0	37.0	35.0	37.0
6	36.68521204974565	37.0	37.0	37.0	35.0	37.0
7	36.57090674747364	37.0	37.0	37.0	35.0	37.0
8	36.582764530857936	37.0	37.0	37.0	35.0	37.0
9	38.3433053159836	39.0	39.0	39.0	37.0	39.0
10	38.18369829016468	39.0	39.0	39.0	35.0	39.0
11	37.70262988304013	39.0	37.0	39.0	35.0	39.0
12	37.65806172958001	39.0	37.0	39.0	35.0	39.0
13	37.64321207716883	39.0	37.0	39.0	35.0	39.0
14	39.02784961127641	41.0	38.0	41.0	35.0	41.0
15	39.071969395730214	41.0	38.0	41.0	35.0	41.0
16	39.07155530569991	41.0	38.0	41.0	35.0	41.0
17	39.10223224691832	41.0	38.0	41.0	35.0	41.0
18	39.091345244134864	41.0	38.0	41.0	35.0	41.0
19	39.02860237758978	41.0	37.0	41.0	35.0	41.0
20	38.958544377562355	41.0	37.0	41.0	35.0	41.0
21	38.91907282225665	41.0	37.0	41.0	35.0	41.0
22	38.88539715621615	41.0	36.0	41.0	35.0	41.0
23	38.88290164676201	41.0	36.0	41.0	35.0	41.0
24	38.83399925957412	41.0	36.0	41.0	35.0	41.0
25	38.79882765902017	41.0	36.0	41.0	35.0	41.0
26	38.74519477314173	41.0	36.0	41.0	35.0	41.0
27	38.685963444899976	41.0	36.0	41.0	35.0	41.0
28	38.70653905746528	41.0	36.0	41.0	35.0	41.0
29	38.716068612798395	41.0	36.0	41.0	35.0	41.0
30	38.61495660281636	40.0	36.0	41.0	35.0	41.0
31	38.583731197981656	41.0	36.0	41.0	35.0	41.0
32	38.47820679820652	40.0	36.0	41.0	35.0	41.0
33	38.37564135964131	40.0	36.0	41.0	35.0	41.0
34	38.2829989990539	40.0	36.0	41.0	35.0	41.0
35	38.23755878844387	40.0	36.0	41.0	35.0	41.0
36	38.19770330860676	40.0	35.0	41.0	35.0	41.0
37	38.15114560337854	40.0	35.0	41.0	35.0	41.0
38	38.118753342200165	40.0	35.0	41.0	35.0	41.0
39	38.05568688212146	40.0	35.0	41.0	35.0	41.0
40	37.97280580274506	40.0	35.0	41.0	35.0	41.0
41	37.94937269473886	40.0	35.0	41.0	35.0	41.0
42	37.8594479713702	40.0	35.0	41.0	35.0	41.0
43	37.78236826589516	40.0	35.0	41.0	35.0	41.0
44	37.697638864131854	40.0	35.0	41.0	34.0	41.0
45	37.635244271983105	40.0	35.0	41.0	34.0	41.0
46	37.639577134551836	40.0	35.0	41.0	35.0	41.0
47	37.56857440594535	40.0	35.0	41.0	34.0	41.0
48	37.57219700813097	40.0	35.0	41.0	34.0	41.0
49	37.51446024324361	40.0	35.0	41.0	34.0	41.0
50	37.4277550013026	39.0	35.0	41.0	34.0	41.0
51	37.345074111146154	39.0	35.0	41.0	34.0	41.0
52	37.11407906103029	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	5.0
15	4.0
16	13.0
17	23.0
18	56.0
19	88.0
20	167.0
21	272.0
22	427.0
23	774.0
24	1353.0
25	2405.0
26	3470.0
27	3876.0
28	4108.0
29	4274.0
30	4980.0
31	5888.0
32	7451.0
33	10225.0
34	27640.0
35	112930.0
36	23537.0
37	36259.0
38	70424.0
39	405736.0
40	2920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.15287052145178	23.32931126681384	26.165965090290822	19.351853121443558
2	25.45543047538084	23.88133989661461	27.488036637369568	23.175192990634983
3	24.785482168076676	25.33134058219413	26.90296307468703	22.980214175042164
4	25.666314735846214	27.343927822188096	23.8396566617762	23.150100780189494
5	22.937571128875238	35.20917031166445	21.497031440676803	20.356227118783508
6	88.41713400337305	1.9578779942685551	7.183227982613703	2.44176001974469
7	89.5383307509838	1.8007431682000796	4.959756482154365	3.7011695986617488
8	79.90360751943618	3.7586211624686348	10.160699839574393	6.177071478520793
9	43.039311129697936	21.489490065952747	14.144876664244284	21.32632214010503
10	32.77001549409716	19.27301147660117	31.402147235057793	16.554825794243875
11	31.846402764256627	19.692586143066734	27.682055641633873	20.778955451042766
12	19.95831676516159	29.595508082982548	31.607135511647993	18.839039640207865
13	16.358338703706245	32.61671991334275	31.65855397567564	19.366387407275372
14	14.743113353717899	25.70607834802759	40.48758415488612	19.06322414336839
15	17.677942164511663	21.641003139954204	41.280251196336266	19.40080349919787
16	17.728400817210787	23.256914069462916	28.588254651657046	30.426430461669252
17	18.986713468895257	24.464219604831964	33.106223690885905	23.442843235386874
18	22.342488105195322	27.8097105483265	29.476765710054707	20.371035636423468
19	24.049992458625276	25.34875430201149	26.85346423331642	23.747789006046812
20	19.763063717760623	29.7652575722258	29.255049293167513	21.21662941684606
21	20.35403326431833	24.21809655701965	29.663380455499034	25.764489723162992
22	17.816154995817964	28.039791035362192	27.246986878007977	26.897067090811866
23	18.591819665162962	23.161207168419466	36.443213448327874	21.8037597180897
24	19.743181911669936	23.20714099628416	32.989949404231396	24.059727687814508
25	17.867710575749683	30.221716416887194	29.427403984588175	22.483169022774952
26	19.305370829962566	27.5457624329846	28.654481633324647	24.49438510372818
27	19.675583770961595	26.900357872509634	34.30036609946387	19.1236922570649
28	18.785290205810973	24.18518874004196	32.20427527388902	24.82524578025805
29	22.838984793846237	25.689213091826517	29.257654495344916	22.214147618982324
30	24.979501172340978	24.99307564684428	27.139350893310116	22.888072287504627
31	28.11163976909682	26.13004072342351	24.933155996764064	20.82516351071561
32	26.276891856686458	26.38960112983505	26.3043150375012	21.029191975977295
33	19.906761185229875	24.741467962869013	28.8675597482552	26.48421110364591
34	21.14697453757661	26.364234687581412	29.317299913616978	23.171490861224996
35	26.671785660418752	25.379605380428078	25.871440128340485	22.077168830812685
36	22.15559912794285	27.099175933416518	26.308565630527486	24.43665930811315
37	20.36637369568496	29.69203767945044	27.04021609466482	22.90137253019978
38	19.615389889073235	28.666273601074987	25.780532283939618	25.937804225912164
39	24.067817526154858	25.215614759155912	26.110707380949115	24.60586033374011
40	17.730594671675966	23.66606792721888	30.603721325636563	27.99961607546859
41	21.506629553961965	26.6266745279785	25.1984752711467	26.66822064691284
42	22.37210514047524	22.787566329818596	29.21898781039613	25.62134071931003
43	24.027368334453115	24.947553166691804	27.426197364632323	23.598881134222758
44	19.829976278948592	28.49789527087247	26.441156709766766	25.230971740412173
45	21.902483169022773	26.645459406836597	27.117549464562394	24.33450795957823
46	23.882162592039048	24.275548120826535	27.347218603885864	24.49507068324855
47	21.610426293345768	21.987495029548477	31.540360066364098	24.861718610741658
48	21.481263111708326	20.84408550547778	28.56837284556636	29.106278537247537
49	21.186601033853915	24.90148222292304	30.119565068352276	23.79235167487077
50	19.782397060235017	25.52152034114437	28.84493562408304	25.851146974537574
51	20.476066418943933	22.730251881915784	29.354595439525035	27.43908625961525
52	21.290946236854012	21.770029205687567	33.266923530460296	23.67210102699812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	207.5
2	338.0
3	1862.5
4	3387.0
5	2272.5
6	1158.0
7	1199.5
8	1241.0
9	1298.0
10	1355.0
11	1289.0
12	1223.0
13	1167.0
14	1061.0
15	1011.0
16	1005.0
17	999.0
18	961.0
19	923.0
20	1125.0
21	1327.0
22	1741.5
23	2156.0
24	2411.0
25	2666.0
26	3505.0
27	4344.0
28	5171.5
29	5999.0
30	7324.5
31	8650.0
32	9837.0
33	11024.0
34	12944.5
35	14865.0
36	16682.5
37	18500.0
38	20274.0
39	23924.5
40	25801.0
41	31626.5
42	37452.0
43	45205.0
44	52958.0
45	57599.0
46	62240.0
47	64523.0
48	66806.0
49	74288.5
50	81771.0
51	82125.0
52	82479.0
53	72888.5
54	63298.0
55	53473.5
56	43649.0
57	37133.0
58	30617.0
59	27492.0
60	24367.0
61	21534.5
62	18702.0
63	16266.5
64	11098.5
65	8366.0
66	6850.5
67	5335.0
68	4076.5
69	2818.0
70	2393.0
71	1968.0
72	1545.0
73	1122.0
74	910.0
75	698.0
76	546.5
77	395.0
78	253.0
79	111.0
80	83.0
81	55.0
82	53.0
83	51.0
84	33.5
85	16.0
86	8.5
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	729310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.326381015761854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.52904821566646	20.976881216674357
2	11.69794352500618	6.8611669783039115
3	4.114207816725583	3.6196447803396046
4	1.9178609373102353	2.249756823312245
5	1.097158736887165	1.6087847576362506
6	0.7657552277874696	1.3474097744944105
7	0.6105162828887386	1.2532963189825261
8	0.4975913333823833	1.1674042426330207
9	0.4235096250657388	1.1178004165668274
>10	6.953192715401622	48.06793230413094
>50	0.3238521674167532	6.136757609470525
>100	0.06327068393465089	3.3448483320429343
>500	0.0032807021299448612	0.637879729988957
>1k	0.0028120303970955956	1.6104367154235313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	2981	0.40874251004374	RNA PCR Primer, Index 34 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT	2395	0.3283925902565439	RNA PCR Primer, Index 34 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	2341	0.3209883314365633	TruSeq Adapter, Index 18 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1725	0.2365249345271558	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1159	0.15891733282143394	TruSeq Adapter, Index 3 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	1116	0.1530213489462643	TruSeq Adapter, Index 18 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	772	0.10585347794490683	TruSeq Adapter, Index 3 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	765	0.10489366661639084	RNA PCR Primer, Index 34 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	738	0.10119153720640056	TruSeq Adapter, Index 18 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33538550136430323	0.0
2	0.0	0.0	0.0	1.3449699030590558	0.0
3	0.0	0.0	0.0	2.181514033812782	0.0
4	0.0	0.0	0.0	2.650861773457103	0.0
5	0.0	0.0	0.0	5.36164319699442	0.0
6	0.0	0.0	0.0	5.766820693532243	0.0
7	0.0	0.0	0.0	7.506958632131741	0.0
8	0.0	0.0	0.0	9.48416996887469	0.0
9	0.0	0.0	0.0	10.928274670579041	0.0
10	0.0	0.0	0.0	12.045357941067584	0.0
11	0.0	0.0	0.0	13.020800482647982	0.0
12	0.0	0.0	0.0	13.793448602103357	0.0
13	0.0	0.0	0.0	14.102644965789581	0.0
14	0.0	0.0	0.0	14.32929755522343	0.0
15	0.0	0.0	0.0	15.241255433217699	0.0
16	0.0	0.0	0.0	16.318575091524867	0.0
17	0.0	0.0	0.0	17.55494919855754	0.0
18	1.371159040737135E-4	0.0	0.0	18.23435850324279	0.0
19	1.371159040737135E-4	0.0	0.0	18.944756002248702	0.0
20	1.371159040737135E-4	0.0	0.0	19.901550780875073	0.0
21	1.371159040737135E-4	0.0	0.0	20.756057095062456	0.0
22	1.371159040737135E-4	0.0	0.0	21.71888497346807	0.0
23	1.371159040737135E-4	0.0	0.0	22.40446449383664	0.0
24	1.371159040737135E-4	0.0	0.0	23.018606628182802	0.0
25	1.371159040737135E-4	0.0	0.0	23.625755851421207	0.0
26	1.371159040737135E-4	0.0	0.0	24.133770276014314	0.0
27	1.371159040737135E-4	0.0	0.0	24.682096776405096	0.0
28	1.371159040737135E-4	0.0	0.0	25.24303793997066	0.0
29	1.371159040737135E-4	0.0	0.0	25.954121018496934	0.0
30	1.371159040737135E-4	0.0	0.0	26.599936926684126	0.0
31	2.74231808147427E-4	0.0	0.0	27.143464370432326	0.0
32	2.74231808147427E-4	0.0	0.0	27.688637205029412	0.0
33	2.74231808147427E-4	0.0	0.0	28.22654289671059	0.0
34	2.74231808147427E-4	0.0	0.0	28.88607039530515	0.0
35	2.74231808147427E-4	0.0	0.0	29.43425977979186	0.0
36	2.74231808147427E-4	0.0	0.0	29.981763584758195	0.0
37	2.74231808147427E-4	0.0	0.0	30.540785125666726	0.0
38	2.74231808147427E-4	0.0	0.0	31.072794833472734	0.0
39	2.74231808147427E-4	0.0	0.0	31.644156805747897	0.0
40	2.74231808147427E-4	0.0	0.0	32.19152349481016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	55	1.8189894E-12	46.000004	1
AACGTCC	20	6.3099293E-4	46.0	21
ACGCCTC	20	6.3099293E-4	46.0	22
CGACGGT	20	6.3099293E-4	46.0	27
TAGGTCG	20	6.3099293E-4	46.0	33
CGCATAT	20	6.3099293E-4	46.0	28
TACGGGT	40	5.6061253E-9	46.0	3
CGCCTAT	20	6.3099293E-4	46.0	36
AATGCGG	80	0.0	46.0	1
CCGTAGG	20	6.3099293E-4	46.0	1
CTCGCTA	20	6.3099293E-4	46.0	38
CGTCGGG	45	3.092282E-10	46.0	2
CCGAACA	20	6.3099293E-4	46.0	29
CACGTTC	20	6.3099293E-4	46.0	46
CCTATCG	20	6.3099293E-4	46.0	38
TCGCGTG	25	3.415919E-5	46.0	44
ATCGCCG	25	3.415919E-5	46.0	21
TATCCGG	65	0.0	46.0	1
CTAGCGG	180	0.0	46.0	1
CCCGAAC	20	6.3099293E-4	46.0	28
>>END_MODULE