FastQCFastQC Report
Thu 26 May 2016
SRR1544995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544995_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences434388
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT23070.5310920191165502RNA PCR Primer, Index 34 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT18800.4327928027477739RNA PCR Primer, Index 34 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT17450.4017145961674816TruSeq Adapter, Index 18 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.2622079799626141No Hit
TGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTTG11130.2562225475841874TruSeq Adapter, Index 18 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC7310.16828273340884187TruSeq Adapter, Index 3 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT7150.16459939040673316TruSeq Adapter, Index 18 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6000.1381253625790768No Hit
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC5900.13582327320275883TruSeq Adapter, Index 18 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC5140.11832739394274244RNA PCR Primer, Index 34 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC5100.11740655819221525RNA PCR Primer, Index 34 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT4970.11441384200300193TruSeq Adapter, Index 3 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGG206.3070963E-446.0000041
TCGGGTA206.3070963E-446.0000044
CAACCGG206.3070963E-446.0000041
TCGATCA206.3070963E-446.00000416
CTAACGG206.3070963E-446.0000041
ACTACGG206.3070963E-446.0000041
CATACGA206.3070963E-446.00000417
ACCACGA206.3070963E-446.00000410
ATACCGG206.3070963E-446.0000041
AAAGCGC206.3070963E-446.00000441
ATCGAGG405.5970304E-946.0000041
CAAACGG301.8587107E-646.01
ATTACGG253.413621E-546.01
TCGATGG301.8587107E-646.01
GTTACGG453.092282E-1046.01
CCGTAGG253.413621E-546.01
GGAACGA253.413621E-546.07
ATGACGG501.6370905E-1146.01
TCCCGTC253.413621E-546.015
CTCGAGG600.046.01
Produced by FastQC (version 0.11.5)