FastQCFastQC Report
Thu 26 May 2016
SRR1544994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544994_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2049509
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.2374227192952068No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT38200.1863861051598212Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT34990.17072381726550115Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33420.16306344592778077No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT32900.16052625287324915Illumina Single End Adapter 1 (95% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27530.13432485536779784No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC26530.1294456379552371No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG21650.10563505698194056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACA206.312618E-446.037
CGGTCTA1450.042.82758330
ACGTAGG2150.042.79071
CGTAGGG7700.041.818182
GCGATCG508.731149E-1041.3999988
CGTTTTT34600.041.2138751
AACACGT4650.041.0537640
TAGACGG1750.040.742861
TATGACC15450.040.64077827
ACGGGTA1700.040.5882344
TCAACGC16400.040.39024415
ATCAACG16500.040.14545414
CGCAAGC16600.040.04216819
AACGCAA16500.039.8666717
CGCACTT14550.039.83505234
TAATCGT2200.039.72727620
TAACGGG4750.039.705262
TCAAGCG5250.039.4285716
CACTTAC14600.039.2260336
ATTGCGG3650.039.0684931