##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544994_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2049509
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89062063157566	34.0	33.0	34.0	31.0	34.0
2	33.244204343576925	34.0	33.0	34.0	31.0	34.0
3	33.32147797350488	34.0	34.0	34.0	31.0	34.0
4	36.59697225042681	37.0	37.0	37.0	35.0	37.0
5	36.58899375411379	37.0	37.0	37.0	35.0	37.0
6	36.64836992665072	37.0	37.0	37.0	35.0	37.0
7	36.471623203411156	37.0	37.0	37.0	35.0	37.0
8	36.50115710640939	37.0	37.0	37.0	35.0	37.0
9	38.314593885657494	39.0	39.0	39.0	37.0	39.0
10	38.16441352538584	39.0	39.0	39.0	35.0	39.0
11	37.76649821981753	39.0	37.0	39.0	35.0	39.0
12	37.70894541082767	39.0	37.0	39.0	35.0	39.0
13	37.6787996539659	39.0	37.0	39.0	35.0	39.0
14	39.09649677068995	41.0	38.0	41.0	35.0	41.0
15	39.14956899432986	41.0	38.0	41.0	35.0	41.0
16	39.14521624447612	41.0	38.0	41.0	35.0	41.0
17	39.16528397777224	41.0	38.0	41.0	35.0	41.0
18	39.13874054712617	41.0	38.0	41.0	35.0	41.0
19	39.08402744267042	41.0	38.0	41.0	35.0	41.0
20	39.01761641446805	41.0	38.0	41.0	35.0	41.0
21	38.9628867206731	41.0	38.0	41.0	35.0	41.0
22	38.91489473820315	41.0	37.0	41.0	35.0	41.0
23	38.884911947202966	41.0	37.0	41.0	35.0	41.0
24	38.81527380460393	41.0	37.0	41.0	35.0	41.0
25	38.762587527061356	41.0	37.0	41.0	35.0	41.0
26	38.69896741121898	41.0	36.0	41.0	35.0	41.0
27	38.618788695243595	41.0	36.0	41.0	35.0	41.0
28	38.62535953733309	41.0	36.0	41.0	35.0	41.0
29	38.61613049759723	40.0	36.0	41.0	35.0	41.0
30	38.55616101222293	40.0	36.0	41.0	35.0	41.0
31	38.50170650629004	40.0	36.0	41.0	35.0	41.0
32	38.42251339223199	40.0	36.0	41.0	35.0	41.0
33	38.32607858760318	40.0	36.0	41.0	35.0	41.0
34	38.20861630761319	40.0	35.0	41.0	35.0	41.0
35	38.12595943711396	40.0	35.0	41.0	35.0	41.0
36	38.0453552533802	40.0	35.0	41.0	35.0	41.0
37	37.993975630260714	40.0	35.0	41.0	35.0	41.0
38	37.87796735705967	40.0	35.0	41.0	35.0	41.0
39	37.77952133901339	40.0	35.0	41.0	35.0	41.0
40	37.6578463427094	40.0	35.0	41.0	34.0	41.0
41	37.59183492241313	40.0	35.0	41.0	34.0	41.0
42	37.50174749171631	40.0	35.0	41.0	34.0	41.0
43	37.406536882736305	39.0	35.0	41.0	34.0	41.0
44	37.326839745519536	39.0	35.0	41.0	34.0	41.0
45	37.264292081664436	39.0	35.0	41.0	34.0	41.0
46	37.2493143479731	39.0	35.0	41.0	34.0	41.0
47	37.179795258278936	39.0	35.0	41.0	34.0	41.0
48	37.119800400974086	39.0	35.0	41.0	34.0	41.0
49	37.06693944744814	39.0	35.0	41.0	34.0	41.0
50	36.990733878211806	38.0	35.0	41.0	34.0	41.0
51	36.90861372162796	38.0	35.0	41.0	34.0	41.0
52	36.68664836309575	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	6.0
13	9.0
14	15.0
15	39.0
16	63.0
17	107.0
18	238.0
19	360.0
20	626.0
21	1075.0
22	1570.0
23	2480.0
24	3982.0
25	6380.0
26	9181.0
27	10792.0
28	11595.0
29	12177.0
30	14015.0
31	16951.0
32	21591.0
33	31219.0
34	100635.0
35	264963.0
36	95392.0
37	159681.0
38	266651.0
39	1009929.0
40	7781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.34890112705043	21.25918939609438	22.38960648623646	20.002302990618727
2	29.76381172271017	23.660545037860288	24.20940820459925	22.366235034830293
3	29.561226615740647	23.977255040109608	23.74705356258499	22.714464781564754
4	27.05891996570886	26.56060549136403	21.06348398567657	25.31699055725054
5	25.19447340802114	36.90410727642572	19.807768592379933	18.09365072317321
6	87.48388028547325	3.3594875650704634	6.637540991525287	2.519091157930997
7	86.6587558288351	3.595446519141902	5.69107039783675	4.054727254186247
8	76.24694499999755	6.116879701430928	11.006538639254574	6.6296366593169385
9	49.06697165028307	20.989759010572776	15.310154773655544	14.633114565488611
10	35.64883101269621	23.5935045906117	23.721584047691422	17.036080349000663
11	32.835913382180806	19.302525629309265	26.737379538221106	21.124181450288827
12	23.83990506994602	24.754221620885783	30.072958937970025	21.332914371198175
13	20.473001094408467	26.030624896011677	30.804500004635255	22.691874004944598
14	18.51165327890729	22.7960940888769	36.63692133091389	22.055331301301923
15	21.686023335345197	20.006206364548778	35.991498451580355	22.31627184852567
16	23.30070275368393	21.879435513579107	27.242720085640027	27.577141647096937
17	23.275672368357494	22.989262306240178	29.309361412904263	24.42570391249807
18	25.124505430325016	25.85267983697559	26.731914814719037	22.290899917980354
19	26.722741885983424	23.508264662414266	25.222187362924487	24.546806088677826
20	25.16827201051569	26.646284549128595	26.124598623377597	22.060844816978115
21	24.33802437559435	22.95276575999422	25.979588281876293	26.729621582535135
22	23.28040520924768	25.7715384514047	25.261904192662733	25.686152146684886
23	22.92168514507621	22.371894927028865	30.888959258046683	23.81746066984824
24	23.309436552852414	21.999317885405727	28.966059675756483	25.725185885985375
25	21.77262944441815	25.266051527463407	27.737716692144314	25.223602335974128
26	22.004245894992412	25.357390477426545	27.148843942622353	25.48951968495869
27	23.27825835358615	24.951439588701486	29.176256361889603	22.594045695822757
28	22.26518644221616	25.20847676199519	26.577487583611493	25.948849212177162
29	23.49411493191784	25.2010603515281	25.89439714585298	25.410427570701078
30	26.089858595400166	24.952464224358124	23.169061467893044	25.788615712348665
31	27.20866314810035	25.43989804387295	23.115780413747878	24.235658394278826
32	25.432335256883476	26.65736037265511	22.907437830231533	25.002866540229878
33	21.798928426271853	26.357922800046257	24.99008299060897	26.85306578307292
34	22.968867177455675	28.024517091654634	25.322991994667994	23.6836237362217
35	27.359040628755473	26.397932382829254	22.86567172918001	23.377355259235262
36	22.927442621623033	28.518196309457533	24.507284427636083	24.04707664128335
37	24.76158923917875	27.34284162694577	24.330071251211876	23.565497882663603
38	24.10030890325439	25.946751148689764	25.01325927331863	24.939680674737215
39	26.714983930297453	23.171891413992327	27.036426773436954	23.076697882273265
40	20.83308733945545	22.373895406168014	30.241145562180993	26.551871692195544
41	22.777260309664413	25.240142883002708	25.067369794423932	26.915227012908943
42	22.97960145576331	23.215657994182997	27.64998836306647	26.154752186987224
43	23.282991194476335	25.589006928000803	27.30444218590892	23.823559691613944
44	21.57770470878635	26.746698843479095	26.253897884810463	25.421698562924096
45	23.611557695038176	25.64248315084247	25.49083707366008	25.25512208045927
46	23.949199539987383	24.11406829635781	27.436425016918687	24.50030714673612
47	23.923046934656057	22.866549988314276	28.556302997449635	24.654100079580036
48	23.014048730695986	22.49636376322329	27.28951178062648	27.20007572545424
49	22.043523595163524	24.686254122328812	28.407486866366526	24.862735416141135
50	22.174384206168405	25.021358774223483	27.535814675612546	25.268442343995563
51	22.646155737788902	22.700022298023576	28.156939052231532	26.49688291195599
52	23.09797127019203	21.179609360095515	29.863298965752282	25.85912040396017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	268.0
1	756.0
2	1244.0
3	5325.5
4	9407.0
5	6388.0
6	3369.0
7	3347.0
8	3325.0
9	3316.5
10	3308.0
11	3238.0
12	3168.0
13	3120.0
14	2921.0
15	2770.0
16	2882.5
17	2995.0
18	3147.0
19	3299.0
20	3450.5
21	3602.0
22	3986.5
23	4371.0
24	4889.0
25	5407.0
26	7455.5
27	9504.0
28	11748.5
29	13993.0
30	16195.5
31	18398.0
32	21309.0
33	24220.0
34	27935.0
35	31650.0
36	34543.5
37	37437.0
38	41401.0
39	53266.0
40	61167.0
41	73892.0
42	86617.0
43	110966.0
44	135315.0
45	154936.0
46	174557.0
47	180374.5
48	186192.0
49	179315.0
50	172438.0
51	156126.0
52	139814.0
53	127930.5
54	116047.0
55	109191.5
56	102336.0
57	100575.5
58	98815.0
59	102964.5
60	107114.0
61	105462.0
62	103810.0
63	99431.0
64	83296.0
65	71540.0
66	60910.5
67	50281.0
68	44703.5
69	39126.0
70	33544.0
71	27962.0
72	23442.0
73	18922.0
74	16238.5
75	13555.0
76	11148.5
77	8742.0
78	7080.5
79	5419.0
80	3943.0
81	2467.0
82	1826.5
83	1186.0
84	803.0
85	420.0
86	369.5
87	319.0
88	211.0
89	57.0
90	11.0
91	8.5
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2049509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.963170264705013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26717671261996	15.872163066887971
2	10.062843796666183	4.4202390330662
3	3.4659558717431884	2.283701368231492
4	1.6950853619892872	1.4891779367431937
5	1.0540895993117312	1.1575574671969118
6	0.7019359614677504	0.9250043421981371
7	0.585780153137024	0.9005912468723404
8	0.4486938964308216	0.788379235523524
9	0.3868929020269128	0.7647655213280627
>10	7.916293785986664	46.07184271648767
>50	1.2700939000052334	17.394738130634416
>100	0.13326722277373784	4.689853212159559
>500	0.006955017190353384	1.0232069414109934
>1k	0.004935818651218531	2.218779781259533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4866	0.2374227192952068	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3820	0.1863861051598212	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3499	0.17072381726550115	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3342	0.16306344592778077	No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	3290	0.16052625287324915	Illumina Single End Adapter 1 (95% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2753	0.13432485536779784	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	2653	0.1294456379552371	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	2165	0.10563505698194056	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16540547028580993	0.0
2	0.0	0.0	0.0	0.62551567229029	0.0
3	4.8792174125607646E-5	0.0	0.0	1.039126932353066	0.0
4	4.8792174125607646E-5	0.0	0.0	1.3174862857396576	0.0
5	4.8792174125607646E-5	0.0	0.0	2.8580503915815934	0.0
6	4.8792174125607646E-5	0.0	0.0	3.264635578570282	0.0
7	4.8792174125607646E-5	0.0	0.0	4.791342706960545	0.0
8	4.8792174125607646E-5	0.0	0.0	6.459498348140945	0.0
9	4.8792174125607646E-5	0.0	0.0	8.025873514095327	0.0
10	9.758434825121529E-5	0.0	0.0	8.766636301670303	0.0
11	9.758434825121529E-5	0.0	0.0	9.448750895946297	0.0
12	9.758434825121529E-5	0.0	0.0	10.020887929743173	0.0
13	9.758434825121529E-5	0.0	0.0	10.288756965692759	0.0
14	9.758434825121529E-5	0.0	0.0	10.461676430793911	0.0
15	1.4637652237682293E-4	0.0	0.0	11.00102512357838	0.0
16	1.4637652237682293E-4	0.0	0.0	11.578138959136066	0.0
17	1.4637652237682293E-4	0.0	0.0	12.245420732477877	0.0
18	1.4637652237682293E-4	0.0	0.0	12.61863207236465	0.0
19	1.4637652237682293E-4	0.0	0.0	13.059664534286016	0.0
20	1.4637652237682293E-4	0.0	0.0	13.574958685226559	0.0
21	1.4637652237682293E-4	0.0	0.0	14.063709893442772	0.0
22	1.9516869650243059E-4	0.0	0.0	14.559145629514191	0.0
23	1.9516869650243059E-4	0.0	0.0	14.958704743428791	0.0
24	1.9516869650243059E-4	0.0	0.0	15.317815144993265	0.0
25	1.9516869650243059E-4	0.0	0.0	15.66145842735992	0.0
26	1.9516869650243059E-4	0.0	0.0	16.004613787985317	0.0
27	1.9516869650243059E-4	0.0	0.0	16.32786194156747	0.0
28	2.4396087062803824E-4	0.0	0.0	16.67389604046628	0.0
29	2.4396087062803824E-4	0.0	0.0	17.089361403145826	0.0
30	2.4396087062803824E-4	0.0	0.0	17.57064740872082	0.0
31	2.4396087062803824E-4	0.0	0.0	17.94678627905513	0.0
32	2.4396087062803824E-4	0.0	0.0	18.32058312503141	0.0
33	2.4396087062803824E-4	0.0	0.0	18.688866455331496	0.0
34	2.4396087062803824E-4	0.0	0.0	19.131753019869638	0.0
35	2.4396087062803824E-4	0.0	0.0	19.4940837049264	0.0
36	2.9275304475364586E-4	0.0	0.0	19.87022257526071	0.0
37	3.4154521887925355E-4	0.0	0.0	20.26075513696207	0.0
38	3.9033739300486117E-4	0.0	0.0	20.741016506880428	0.0
39	3.9033739300486117E-4	0.0	0.0	21.133842300765696	0.0
40	4.391295671304688E-4	0.0	0.0	21.50149133280215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACA	20	6.312618E-4	46.0	37
CGGTCTA	145	0.0	42.827583	30
ACGTAGG	215	0.0	42.7907	1
CGTAGGG	770	0.0	41.81818	2
GCGATCG	50	8.731149E-10	41.399998	8
CGTTTTT	3460	0.0	41.213875	1
AACACGT	465	0.0	41.05376	40
TAGACGG	175	0.0	40.74286	1
TATGACC	1545	0.0	40.640778	27
ACGGGTA	170	0.0	40.588234	4
TCAACGC	1640	0.0	40.390244	15
ATCAACG	1650	0.0	40.145454	14
CGCAAGC	1660	0.0	40.042168	19
AACGCAA	1650	0.0	39.86667	17
CGCACTT	1455	0.0	39.835052	34
TAATCGT	220	0.0	39.727276	20
TAACGGG	475	0.0	39.70526	2
TCAAGCG	525	0.0	39.42857	16
CACTTAC	1460	0.0	39.22603	36
ATTGCGG	365	0.0	39.068493	1
>>END_MODULE