FastQCFastQC Report
Thu 26 May 2016
SRR1544993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544993_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1251164
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA49790.3979494294912577No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT34240.2736651630002142Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.25823952735212974No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT27270.2179570384058365Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT24580.19645705918648554Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC20240.16176936037162196No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16240.12979913104916702No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTTG15930.12732143827667677Illumina Single End Adapter 1 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC15620.1248437455041865No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG13330.10654078921708106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA1050.046.030
TACGGGT601.8189894E-1242.1666683
CGTTTTT20700.041.5555571
TCAACGC9500.041.39999815
AACGCAA9550.040.94240617
CGTAGGG4050.040.888892
CATGCGG2700.040.8888851
CGCACTT8700.040.71264334
CACTTAC8700.040.44827736
TATACGG800.040.251
TCGATGG1150.040.01
ACTACGG700.039.428571
CCGCACT9000.039.35555333
TATGACC9650.039.32642727
CTCGAGG1350.039.1851841
ATCAACG10050.039.1343314
CGCAAGC10150.038.9753719
CTTATGA10000.038.8725
GCTTATG10050.038.6766224
ACGCAAG10150.038.52216718
Produced by FastQC (version 0.11.5)