##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544993_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1251164
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.848152600298604	34.0	33.0	34.0	31.0	34.0
2	33.24984094810912	34.0	33.0	34.0	31.0	34.0
3	33.38960360112663	34.0	34.0	34.0	31.0	34.0
4	36.60615394944228	37.0	37.0	37.0	35.0	37.0
5	36.613089890693786	37.0	37.0	37.0	35.0	37.0
6	36.402366116672155	37.0	37.0	37.0	35.0	37.0
7	36.19606382536582	37.0	37.0	37.0	35.0	37.0
8	36.339212125668574	37.0	37.0	37.0	35.0	37.0
9	38.22895399803703	39.0	39.0	39.0	37.0	39.0
10	38.15371286258236	39.0	39.0	39.0	35.0	39.0
11	37.79366493920861	39.0	37.0	39.0	35.0	39.0
12	37.773539679850124	39.0	38.0	39.0	35.0	39.0
13	37.75133156005128	39.0	38.0	39.0	35.0	39.0
14	39.07728163534117	41.0	38.0	41.0	35.0	41.0
15	39.10997599035778	41.0	38.0	41.0	35.0	41.0
16	39.12925883417362	41.0	38.0	41.0	35.0	41.0
17	39.17909402764146	41.0	38.0	41.0	35.0	41.0
18	39.1771270592824	41.0	38.0	41.0	35.0	41.0
19	39.155225054429316	41.0	38.0	41.0	35.0	41.0
20	39.102826647825545	41.0	38.0	41.0	35.0	41.0
21	39.0448638228082	41.0	38.0	41.0	35.0	41.0
22	38.99492152907213	41.0	38.0	41.0	35.0	41.0
23	38.94962371040087	41.0	38.0	41.0	35.0	41.0
24	38.895102480570095	41.0	37.0	41.0	35.0	41.0
25	38.830329996707064	41.0	37.0	41.0	35.0	41.0
26	38.7429505644344	41.0	37.0	41.0	35.0	41.0
27	38.788245985338456	41.0	37.0	41.0	35.0	41.0
28	38.77203707907197	41.0	37.0	41.0	35.0	41.0
29	38.77529084916126	41.0	37.0	41.0	35.0	41.0
30	38.72823386862154	41.0	37.0	41.0	35.0	41.0
31	38.671079890406055	41.0	36.0	41.0	35.0	41.0
32	38.592535431006645	41.0	36.0	41.0	35.0	41.0
33	38.50068336365177	40.0	36.0	41.0	35.0	41.0
34	38.37679872502726	40.0	36.0	41.0	35.0	41.0
35	38.28256087930919	40.0	35.0	41.0	35.0	41.0
36	38.20771457618665	40.0	35.0	41.0	35.0	41.0
37	38.164863279314304	40.0	35.0	41.0	35.0	41.0
38	38.03354556237232	40.0	35.0	41.0	35.0	41.0
39	37.956748276005385	40.0	35.0	41.0	35.0	41.0
40	37.85339092237309	40.0	35.0	41.0	35.0	41.0
41	37.79706737086425	40.0	35.0	41.0	35.0	41.0
42	37.734540795611125	40.0	35.0	41.0	35.0	41.0
43	37.66464188547624	40.0	35.0	41.0	35.0	41.0
44	37.5290969049621	40.0	35.0	41.0	35.0	41.0
45	37.50611750338085	39.0	35.0	41.0	35.0	41.0
46	37.423878084727505	39.0	35.0	41.0	35.0	41.0
47	37.31844985949084	39.0	35.0	41.0	34.0	41.0
48	37.20015761323056	39.0	35.0	41.0	34.0	41.0
49	37.157801854912705	39.0	35.0	41.0	34.0	41.0
50	37.08248878644206	39.0	35.0	41.0	34.0	41.0
51	36.98369997857995	38.0	35.0	41.0	34.0	41.0
52	36.479109852904976	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	6.0
14	14.0
15	23.0
16	33.0
17	72.0
18	130.0
19	228.0
20	368.0
21	592.0
22	869.0
23	1377.0
24	2125.0
25	3579.0
26	5205.0
27	6200.0
28	6307.0
29	6502.0
30	7175.0
31	8722.0
32	11556.0
33	17160.0
34	61113.0
35	155346.0
36	58089.0
37	99219.0
38	170823.0
39	625556.0
40	2770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.244540284087456	21.062626482219756	21.735120256017595	21.95771297767519
2	29.463443641281238	23.988302093090912	24.20418106659079	22.344073199037055
3	29.64863119463156	23.822376602907372	23.769705650098626	22.75928655236244
4	26.879369930720515	26.233331521687003	21.70586749618755	25.181431051404935
5	25.16360764855766	36.144502239514566	19.975318982963064	18.716571128964706
6	86.36725481231878	3.3247439983887004	7.20073467586983	3.107266513422701
7	85.61315702817537	3.471327499832156	6.254176111205245	4.6613393607872355
8	74.86092950244732	6.1065535773088095	11.660102112912456	7.372414807331413
9	49.14935212330278	20.235636575221154	15.561429197131632	15.053582104344434
10	35.04480627639542	23.127743445303732	24.61036283013258	17.217087448168268
11	32.04008427352449	18.91118989996515	27.03186792458862	22.01685790192173
12	23.450722687033835	23.92524081575237	30.543797615660296	22.080238881553495
13	20.322595598978232	25.17359834522093	31.13796432761812	23.365841728182716
14	18.22718684361123	22.411050829467598	36.35374739043003	23.00801493649114
15	21.374336218113694	19.48417633499685	36.032926139179196	23.10856130771026
16	23.075552045934824	22.170714630536047	27.44076715762282	27.31296616590631
17	22.839451902388497	23.03407067338894	29.48166667199504	24.644810752227524
18	24.603409305254946	25.84625197016538	26.781780805713716	22.76855791886595
19	26.19800441828569	23.406044291555702	25.47307946839903	24.922871821759575
20	24.600611910189233	26.883845762825658	26.222381718144067	22.29316060884105
21	23.990460083570177	22.637959532083723	25.992355918169004	27.379224466177092
22	22.990511235937095	25.770402601097857	25.40738064714138	25.831705515823668
23	22.514634372472354	22.341835282984484	30.949659676908865	24.1938706676343
24	23.148763871083247	22.226822382996954	28.563481685854132	26.060932060065667
25	21.77500311709736	24.94804837735101	27.770060519644108	25.50688798590752
26	21.998155317768095	24.986092950244732	26.847959180411202	26.167792551575975
27	23.789047638838714	24.597414887256985	28.88550182070456	22.72803565319974
28	22.521507971776682	25.021420053646043	26.231013680061128	26.226058294516147
29	23.63790837971681	24.871559603697037	25.54005709882957	25.950474917756583
30	25.929214715257153	25.0143066776218	22.61238334862576	26.444095258495288
31	27.10811692152268	25.253443992953763	22.53685368185146	25.1015854036721
32	24.951724953723094	26.764516881879597	22.692948326518348	25.590809837878968
33	21.306879034243313	26.365768196655274	24.79283291399049	27.534519855110922
34	22.999782602440607	28.24154147657701	25.034447922094945	23.724227998887436
35	27.17157782672775	26.312457839260077	22.949109788964517	23.56685454504765
36	23.114875428001444	28.540702897461884	24.01603626702814	24.32838540750853
37	24.931024230236805	27.437170506824042	24.059276002186763	23.572529260752386
38	24.028584582037208	25.74634500353271	24.71690361934966	25.508166795080424
39	27.030828892135645	22.71540741261737	27.018360502699885	23.2354031925471
40	20.805905540760445	21.855408243843332	30.404807043680925	26.933879171715297
41	22.643074768775318	25.077607731680256	25.211562992541346	27.067754507003077
42	22.708054259873204	22.82474559690017	27.665198167466453	26.80200197576017
43	23.507150141787967	25.311150256880794	27.194116838400085	23.987582762931158
44	21.69547717165775	26.08858630842959	26.620810701075158	25.5951258188375
45	23.48724867403474	24.98297585288579	25.685841344539966	25.8439341285395
46	24.032181232835985	23.29310945647413	27.552183406811576	25.122525903878305
47	23.690659258098858	22.529660380253908	28.711343996470482	25.068336365176748
48	22.827463066392575	21.787711283253035	27.1643845251302	28.220441125224195
49	22.023571650079447	23.853947204363298	28.58322330246075	25.539257843096507
50	22.27030189487549	24.319273892151628	27.601417559968155	25.809006653004722
51	22.78781998203273	22.00438951248597	28.195264569632755	27.012525935848537
52	22.962137657413418	20.999405353734602	30.083186536697028	25.955270452154956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	512.5
2	844.0
3	3485.0
4	6126.0
5	4137.5
6	2149.0
7	2182.0
8	2215.0
9	2162.0
10	2109.0
11	2080.5
12	2052.0
13	2010.0
14	1837.5
15	1707.0
16	1761.5
17	1816.0
18	1870.5
19	1925.0
20	2019.5
21	2114.0
22	2290.5
23	2467.0
24	2775.5
25	3084.0
26	4149.5
27	5215.0
28	6322.0
29	7429.0
30	8698.5
31	9968.0
32	11442.0
33	12916.0
34	15218.5
35	17521.0
36	19157.0
37	20793.0
38	23102.5
39	29880.5
40	34349.0
41	41735.0
42	49121.0
43	64303.5
44	79486.0
45	94594.5
46	109703.0
47	114125.5
48	118548.0
49	114069.5
50	109591.0
51	98418.0
52	87245.0
53	79681.0
54	72117.0
55	67551.0
56	62985.0
57	61370.5
58	59756.0
59	62183.5
60	64611.0
61	63829.5
62	63048.0
63	62142.0
64	52804.0
65	44372.0
66	37892.5
67	31413.0
68	27792.0
69	24171.0
70	20904.5
71	17638.0
72	14724.5
73	11811.0
74	10025.0
75	8239.0
76	6846.0
77	5453.0
78	4430.5
79	3408.0
80	2502.5
81	1597.0
82	1152.5
83	708.0
84	492.5
85	277.0
86	230.5
87	184.0
88	128.0
89	40.0
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1251164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.940899123132457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33202082141152	19.48689676321964
2	9.106667649511337	4.906836289867574
3	3.117364193052648	2.5195378276528975
4	1.6292848057676228	1.755775903801519
5	1.134926972148356	1.5287976534385506
6	0.879774527239178	1.4221150073671343
7	0.7385078925095897	1.3927246643616602
8	0.6730898886468762	1.4506917432668758
9	0.6328806288018766	1.5345335859782392
>10	9.448974261875348	53.37846271030522
>50	0.22950903691176097	4.148589076348462
>100	0.06774746559432002	3.2363535253805886
>500	0.0056704927116563615	1.0584422199103671
>1k	0.0035813638178882282	2.1802430291013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4979	0.3979494294912577	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3424	0.2736651630002142	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3231	0.25823952735212974	No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	2727	0.2179570384058365	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	2458	0.19645705918648554	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2024	0.16176936037162196	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1624	0.12979913104916702	No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTTG	1593	0.12732143827667677	Illumina Single End Adapter 1 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	1562	0.1248437455041865	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	1333	0.10654078921708106	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2225127960842863	0.0
2	0.0	0.0	0.0	0.8040512674597415	0.0
3	0.0	0.0	0.0	1.285043367616076	0.0
4	0.0	0.0	0.0	1.612818143744545	0.0
5	0.0	0.0	0.0	3.3830097413288747	0.0
6	7.992557330613732E-5	0.0	0.0	3.842342011119246	0.0
7	1.5985114661227465E-4	0.0	0.0	5.604940679239492	0.0
8	1.5985114661227465E-4	0.0	0.0	7.509806867844663	0.0
9	1.5985114661227465E-4	0.0	0.0	9.352411034844353	0.0
10	1.5985114661227465E-4	0.0	0.0	10.232551448091536	0.0
11	2.3977671991841197E-4	0.0	0.0	11.005431741961885	0.0
12	2.3977671991841197E-4	0.0	0.0	11.678165292479642	0.0
13	2.3977671991841197E-4	0.0	0.0	11.98779696346762	0.0
14	2.3977671991841197E-4	0.0	0.0	12.179538413829043	0.0
15	2.3977671991841197E-4	0.0	0.0	12.769469070401643	0.0
16	2.3977671991841197E-4	0.0	0.0	13.409353210290577	0.0
17	2.3977671991841197E-4	0.0	0.0	14.145547666013409	0.0
18	2.3977671991841197E-4	0.0	0.0	14.571471046161815	0.0
19	2.3977671991841197E-4	0.0	0.0	15.036238254937002	0.0
20	3.197022932245493E-4	0.0	0.0	15.602510941810985	0.0
21	3.996278665306866E-4	0.0	0.0	16.133056897417124	0.0
22	3.996278665306866E-4	0.0	0.0	16.67902848867135	0.0
23	3.996278665306866E-4	0.0	0.0	17.107909115032083	0.0
24	3.996278665306866E-4	0.0	0.0	17.497066731459665	0.0
25	3.996278665306866E-4	0.0	0.0	17.860248536562754	0.0
26	3.996278665306866E-4	0.0	0.0	18.235099475368536	0.0
27	3.996278665306866E-4	0.0	0.0	18.59076827658085	0.0
28	3.996278665306866E-4	0.0	0.0	18.9469965568063	0.0
29	3.996278665306866E-4	0.0	0.0	19.36292924029144	0.0
30	3.996278665306866E-4	0.0	0.0	19.858228018069575	0.0
31	3.996278665306866E-4	0.0	0.0	20.2609729819592	0.0
32	3.996278665306866E-4	0.0	0.0	20.644935436121884	0.0
33	3.996278665306866E-4	0.0	0.0	21.015310542822522	0.0
34	3.996278665306866E-4	0.0	0.0	21.475202291626037	0.0
35	3.996278665306866E-4	0.0	0.0	21.856766978589537	0.0
36	3.996278665306866E-4	0.0	0.0	22.256155068400307	0.0
37	3.996278665306866E-4	0.0	0.0	22.664894450287893	0.0
38	3.996278665306866E-4	0.0	0.0	23.17993484467264	0.0
39	3.996278665306866E-4	0.0	0.0	23.57996233906986	0.0
40	3.996278665306866E-4	0.0	0.0	23.97015898795042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	105	0.0	46.0	30
TACGGGT	60	1.8189894E-12	42.166668	3
CGTTTTT	2070	0.0	41.555557	1
TCAACGC	950	0.0	41.399998	15
AACGCAA	955	0.0	40.942406	17
CGTAGGG	405	0.0	40.88889	2
CATGCGG	270	0.0	40.888885	1
CGCACTT	870	0.0	40.712643	34
CACTTAC	870	0.0	40.448277	36
TATACGG	80	0.0	40.25	1
TCGATGG	115	0.0	40.0	1
ACTACGG	70	0.0	39.42857	1
CCGCACT	900	0.0	39.355553	33
TATGACC	965	0.0	39.326427	27
CTCGAGG	135	0.0	39.185184	1
ATCAACG	1005	0.0	39.13433	14
CGCAAGC	1015	0.0	38.97537	19
CTTATGA	1000	0.0	38.87	25
GCTTATG	1005	0.0	38.67662	24
ACGCAAG	1015	0.0	38.522167	18
>>END_MODULE