FastQCFastQC Report
Thu 26 May 2016
SRR1544992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544992_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences482681
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT25960.5378293324162335No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT21910.45392298433126643No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT21870.4530942796588223Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.2506831634143461No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT9180.1901877223259254No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC8750.18127914709715112No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC6630.1373577994576128No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC6340.13134969058239293No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT6050.12534158170717305No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC5960.1234769961941738No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG5870.12161241068117452No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT5030.10420961255984801No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATT206.3077966E-446.00000414
AGTCCGT206.3077966E-446.00000436
CTATCGA206.3077966E-446.00000417
ACGGGTA405.5988494E-946.0000044
TCGGCTT206.3077966E-446.00000414
TCGTGCA206.3077966E-446.00000422
AGTTCGG206.3077966E-446.0000041
GCTAGAT206.3077966E-446.00000413
TATCGAA206.3077966E-446.00000418
ATACCGG206.3077966E-446.0000041
CATGCGG1000.046.01
ACTCTAA301.8591436E-646.033
CAACCGG253.41419E-546.01
CCTACGG253.41419E-546.01
TCGATCA301.8591436E-646.016
ACGGGCG453.092282E-1046.04
AACGGCT301.8591436E-646.03
ACTACGG301.8591436E-646.01
CGCAGAT301.8591436E-646.027
TTAGTGG301.8591436E-646.01
Produced by FastQC (version 0.11.5)