##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544992_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	482681
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84342868271177	34.0	31.0	34.0	31.0	34.0
2	33.134113420664995	34.0	33.0	34.0	31.0	34.0
3	33.18126878828875	34.0	33.0	34.0	31.0	34.0
4	36.50307553021561	37.0	37.0	37.0	35.0	37.0
5	36.48574317199144	37.0	37.0	37.0	35.0	37.0
6	36.614745556589135	37.0	37.0	37.0	35.0	37.0
7	36.425479768211304	37.0	37.0	37.0	35.0	37.0
8	36.46694607825872	37.0	37.0	37.0	35.0	37.0
9	38.22584481261951	39.0	39.0	39.0	37.0	39.0
10	38.06944130802746	39.0	39.0	39.0	35.0	39.0
11	37.35187836272818	39.0	35.0	39.0	35.0	39.0
12	37.2310117862522	39.0	35.0	39.0	35.0	39.0
13	37.21018229431032	39.0	35.0	39.0	35.0	39.0
14	38.5139129155695	41.0	35.0	41.0	35.0	41.0
15	38.601065714208765	41.0	35.0	41.0	35.0	41.0
16	38.6183794265778	41.0	35.0	41.0	35.0	41.0
17	38.68326700243018	41.0	36.0	41.0	35.0	41.0
18	38.650241049471596	40.0	36.0	41.0	35.0	41.0
19	38.56465035913989	40.0	35.0	41.0	35.0	41.0
20	38.46775406531436	40.0	35.0	41.0	35.0	41.0
21	38.40968880067788	40.0	35.0	41.0	35.0	41.0
22	38.37827260654552	40.0	35.0	41.0	35.0	41.0
23	38.36224131465709	40.0	35.0	41.0	35.0	41.0
24	38.30707030108913	40.0	35.0	41.0	35.0	41.0
25	38.27414586445292	40.0	35.0	41.0	35.0	41.0
26	38.23821737337911	40.0	35.0	41.0	35.0	41.0
27	38.189124908583516	40.0	35.0	41.0	35.0	41.0
28	38.22768246523066	40.0	35.0	41.0	35.0	41.0
29	38.24584145636559	40.0	35.0	41.0	35.0	41.0
30	38.18011896055573	40.0	35.0	41.0	35.0	41.0
31	38.1090078126133	40.0	35.0	41.0	35.0	41.0
32	38.03517229806021	40.0	35.0	41.0	35.0	41.0
33	37.94403135818481	40.0	35.0	41.0	35.0	41.0
34	37.86332381013547	40.0	35.0	41.0	35.0	41.0
35	37.790256919166076	40.0	35.0	41.0	35.0	41.0
36	37.716705235963296	40.0	35.0	41.0	35.0	41.0
37	37.64901456655638	40.0	35.0	41.0	34.0	41.0
38	37.61563641411201	40.0	35.0	41.0	35.0	41.0
39	37.50489453697163	40.0	35.0	41.0	34.0	41.0
40	37.43471153826233	40.0	35.0	41.0	34.0	41.0
41	37.441395041445595	40.0	35.0	41.0	34.0	41.0
42	37.33490027575148	39.0	35.0	41.0	34.0	41.0
43	37.2496244931953	39.0	35.0	41.0	34.0	41.0
44	37.175295899362105	39.0	35.0	41.0	34.0	41.0
45	37.13951035984429	39.0	35.0	41.0	34.0	41.0
46	37.17136576745304	39.0	35.0	41.0	34.0	41.0
47	37.11061135615448	39.0	35.0	41.0	34.0	41.0
48	37.14532786664484	39.0	35.0	41.0	34.0	41.0
49	37.06483785357203	39.0	35.0	41.0	34.0	41.0
50	36.96312471383792	39.0	35.0	41.0	34.0	41.0
51	36.88847914046752	39.0	35.0	41.0	33.0	41.0
52	36.69336476886391	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	10.0
15	9.0
16	23.0
17	14.0
18	40.0
19	95.0
20	118.0
21	227.0
22	386.0
23	635.0
24	1170.0
25	1956.0
26	2715.0
27	2972.0
28	3001.0
29	3194.0
30	3661.0
31	4660.0
32	5955.0
33	8473.0
34	23970.0
35	98949.0
36	16643.0
37	24506.0
38	44562.0
39	232603.0
40	2131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.655068254188585	22.646426936216674	24.861761701827913	20.83674310776683
2	26.611985970029895	23.777194461766673	27.251745977156755	22.359073591046673
3	26.6534212036521	24.47662120530951	26.33001920523078	22.539938385807602
4	26.274910344513252	26.91653493715311	23.93526987803539	22.87328484029825
5	22.922799944476786	35.44162707875388	21.351575885522735	20.2839970912466
6	84.83429014193639	2.610419718198976	9.168995672089848	3.386294467774783
7	84.58091368833661	2.7515066886825874	6.734054168280915	5.933525454699895
8	73.35714478092156	4.913182826753073	13.968231606381853	7.7614407859435115
9	47.79719939255948	18.31188714699771	17.325935762957315	16.564977697485503
10	40.351909439153395	19.726900375196042	27.115216882371584	12.805973303278979
11	38.69180680407972	16.46408290361543	25.528868963145428	19.315241329159424
12	19.148464513830046	33.324700992995375	30.43521497635084	17.09161951682374
13	13.05603493818899	36.67515398368695	30.188468160130604	20.08034291799346
14	11.773614457581715	23.779887751952117	46.1973850224061	18.249112768060062
15	12.504324802509318	17.387674261054404	49.1651836305966	20.942817305839675
16	13.306718101603337	21.77442244463735	30.07327821066087	34.845581243098444
17	16.694462802554895	22.961956240249773	35.60032402352692	24.743256933668405
18	21.812957211906	27.91512406744827	29.21246123215954	21.059457488486185
19	24.51080527304783	22.299821206966918	26.91301294229522	26.276360577690028
20	17.287815348024886	31.3059764109215	28.979595219202746	22.42661302185087
21	18.876442205100265	23.805163244461664	26.933523382938212	30.38487116749986
22	16.569328397015834	30.40890360300074	23.66780544500405	29.353962554979375
23	16.497645442849418	22.541181442816267	38.88344475958242	22.077728354751898
24	17.933790640195078	21.864336901597536	35.13894269714366	25.062929761063724
25	14.79424298864053	29.712170149643345	30.426513577290176	25.067073284425945
26	16.66317920117013	29.35914195918215	26.975787321232865	27.001891518414855
27	18.62202987065992	26.587124829856574	34.64317012685397	20.14767517262954
28	14.466490290688883	24.53856687957471	31.73628131208811	29.258661517648303
29	17.502449858187912	26.981795430108086	27.77797344415877	27.73778126754523
30	23.900878634128958	24.47434226746029	22.037536178138357	29.587242920272395
31	24.96949330924565	23.355383783492616	25.787631997116105	25.887490910145623
32	21.275127879489766	27.642065877877936	21.99030001180904	29.092506230823258
33	15.178347604318379	24.505833045013166	26.637468638707553	33.6783507119609
34	18.644404896815907	24.616465118784454	28.181138267302835	28.557991717096797
35	23.66221168846505	22.460382737252967	25.35981320996683	28.51759236431515
36	20.715752225589988	29.422537866624126	24.81991211586949	25.041797791916398
37	19.92247467789285	25.42030865105525	26.375805138383324	28.281411532668578
38	18.696820467348	27.889434222602503	24.976744475129536	28.437000834919957
39	23.607724356251854	24.024769982659354	25.452213780944348	26.915291880144444
40	14.905703767084264	22.088501515493668	30.513320391728698	32.492474325693365
41	20.834671346085717	24.009438946219138	24.463776282886627	30.692113424808515
42	20.385927765957227	21.937055736604506	28.90666920802766	28.7703472894106
43	21.75701964651602	26.88918768296245	27.35367665186738	24.00011601865414
44	16.199518936937647	30.22555269422248	25.867187645670743	27.707740723169135
45	21.096127670241838	25.378873417433045	25.875474692395183	27.649524219929933
46	23.4019984213176	22.277446180810927	27.38744636726948	26.933109030601994
47	19.898856594728194	20.291248257130487	31.351347991737814	28.4585471564035
48	19.915016335840853	19.113658917587394	27.570175747543406	33.401148999028344
49	20.407474087440775	22.98557432341443	30.731891249085834	25.875060340058965
50	18.770160830859307	23.638800781468504	27.933148394073932	29.657889993598257
51	18.441993780571433	20.642826214414903	30.25455735775802	30.660622647255643
52	19.593686099100648	18.609599300573258	35.554538090374386	26.242176509951708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	149.5
2	206.0
3	1136.0
4	2066.0
5	1404.5
6	743.0
7	825.5
8	908.0
9	955.5
10	1003.0
11	1008.0
12	1013.0
13	960.5
14	857.0
15	806.0
16	835.0
17	864.0
18	803.5
19	743.0
20	820.0
21	897.0
22	833.5
23	770.0
24	920.5
25	1071.0
26	1368.5
27	1666.0
28	2216.0
29	2766.0
30	3079.5
31	3393.0
32	3905.5
33	4418.0
34	5383.0
35	6348.0
36	7842.0
37	9336.0
38	9635.0
39	11704.5
40	13475.0
41	18746.0
42	24017.0
43	30187.0
44	36357.0
45	39982.0
46	43607.0
47	45714.5
48	47822.0
49	53891.5
50	59961.0
51	61319.0
52	62677.0
53	53999.5
54	45322.0
55	36993.0
56	28664.0
57	24065.5
58	19467.0
59	17066.5
60	14666.0
61	13418.0
62	12170.0
63	10974.0
64	7669.0
65	5560.0
66	4409.5
67	3259.0
68	2688.0
69	2117.0
70	1822.0
71	1527.0
72	1274.0
73	1021.0
74	799.5
75	578.0
76	448.0
77	318.0
78	290.0
79	262.0
80	154.0
81	46.0
82	40.5
83	35.0
84	21.5
85	8.0
86	10.5
87	13.0
88	6.5
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	482681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.199571472343866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17175349117565	21.191568848964547
2	12.254668981617467	7.401715035605442
3	4.447700004156455	4.02955902489201
4	2.1456146419562163	2.59186570927457
5	1.2174692135704148	1.8383524265299014
6	0.8356659562309326	1.5142052263320378
7	0.5883182555406046	1.2436871444678206
8	0.49621904931083183	1.1988482116480794
9	0.4444582042343648	1.2080202574720775
>10	7.025242752282895	45.149627812574
>50	0.28979622075136363	5.8263239077227515
>100	0.07485257834573135	3.9930248636468235
>500	0.005493767218035329	1.115948207711829
>1k	0.0027468836090176643	1.6972533231580766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	2596	0.5378293324162335	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	2191	0.45392298433126643	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	2187	0.4530942796588223	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1210	0.2506831634143461	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	918	0.1901877223259254	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	875	0.18127914709715112	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	663	0.1373577994576128	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	634	0.13134969058239293	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	605	0.12534158170717305	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	596	0.1234769961941738	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	587	0.12161241068117452	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	503	0.10420961255984801	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4622100310557076	0.0
2	0.0	0.0	0.0	1.7918666779922972	0.0
3	0.0	0.0	0.0	2.845979021341217	0.0
4	0.0	0.0	0.0	3.5012772410764046	0.0
5	0.0	0.0	0.0	7.048340415305346	0.0
6	0.0	0.0	0.0	7.71296156260553	0.0
7	0.0	0.0	0.0	10.897673618808282	0.0
8	0.0	0.0	0.0	14.221815236149755	0.0
9	0.0	0.0	0.0	17.21178169432814	0.0
10	0.0	0.0	0.0	18.544753159954503	0.0
11	0.0	0.0	0.0	19.926825377423185	0.0
12	0.0	0.0	0.0	21.156415935162148	0.0
13	0.0	0.0	0.0	21.584649074647643	0.0
14	0.0	0.0	0.0	21.910951539422516	0.0
15	0.0	0.0	0.0	23.074452899534062	0.0
16	0.0	0.0	0.0	24.268409156357926	0.0
17	0.0	0.0	0.0	26.05696101566045	0.0
18	0.0	0.0	0.0	26.773376204988388	0.0
19	0.0	0.0	0.0	27.695724505418692	0.0
20	0.0	0.0	0.0	28.76309612352672	0.0
21	0.0	0.0	0.0	29.673013853870362	0.0
22	0.0	0.0	0.0	30.739763943474053	0.0
23	0.0	0.0	0.0	31.419716127214453	0.0
24	0.0	0.0	0.0	31.989036237183566	0.0
25	0.0	0.0	0.0	32.60414228030521	0.0
26	0.0	0.0	0.0	33.14715101692422	0.0
27	0.0	0.0	0.0	33.687259287189676	0.0
28	0.0	0.0	0.0	34.21866615839447	0.0
29	0.0	0.0	0.0	34.958285078550844	0.0
30	0.0	0.0	0.0	35.67138544918901	0.0
31	0.0	0.0	0.0	36.24547061102467	0.0
32	0.0	0.0	0.0	36.79655921820001	0.0
33	0.0	0.0	0.0	37.28611650344637	0.0
34	0.0	0.0	0.0	38.014962262860976	0.0
35	0.0	0.0	0.0	38.528137631272	0.0
36	0.0	0.0	0.0	39.09372857021511	0.0
37	0.0	0.0	0.0	39.60856134797102	0.0
38	2.0717616811102984E-4	0.0	0.0	40.12733047292104	0.0
39	2.0717616811102984E-4	0.0	0.0	40.69105682635115	0.0
40	2.0717616811102984E-4	0.0	0.0	41.246703309224934	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATT	20	6.3077966E-4	46.000004	14
AGTCCGT	20	6.3077966E-4	46.000004	36
CTATCGA	20	6.3077966E-4	46.000004	17
ACGGGTA	40	5.5988494E-9	46.000004	4
TCGGCTT	20	6.3077966E-4	46.000004	14
TCGTGCA	20	6.3077966E-4	46.000004	22
AGTTCGG	20	6.3077966E-4	46.000004	1
GCTAGAT	20	6.3077966E-4	46.000004	13
TATCGAA	20	6.3077966E-4	46.000004	18
ATACCGG	20	6.3077966E-4	46.000004	1
CATGCGG	100	0.0	46.0	1
ACTCTAA	30	1.8591436E-6	46.0	33
CAACCGG	25	3.41419E-5	46.0	1
CCTACGG	25	3.41419E-5	46.0	1
TCGATCA	30	1.8591436E-6	46.0	16
ACGGGCG	45	3.092282E-10	46.0	4
AACGGCT	30	1.8591436E-6	46.0	3
ACTACGG	30	1.8591436E-6	46.0	1
CGCAGAT	30	1.8591436E-6	46.0	27
TTAGTGG	30	1.8591436E-6	46.0	1
>>END_MODULE