FastQCFastQC Report
Thu 26 May 2016
SRR1544991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544991_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences304507
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT23250.7635292456331053No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT19930.6545005533534533Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT14350.47125353440150797No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTG10390.3412072628872243Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.2554949475709918No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT6230.20459299786211813No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6180.2029509994844125No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC5720.18784461440952097No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC5050.1658418361482659No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC5000.1641998377705603No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT4630.15204904977553882No Hit
CTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTGAAA4000.1313598702164482Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC3950.12971787183874264No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG3380.11099909033289874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGT551.8189894E-1246.00000440
CGTGAGT551.8189894E-1246.00000431
TCACGAC206.3041114E-446.024
ATGATAG206.3041114E-446.019
ATAGGGT206.3041114E-446.03
CGACGGT206.3041114E-446.027
CATGCGG405.5879354E-946.01
GCGCGAC253.4112003E-546.08
TTTCGGG253.4112003E-546.02
ATAATGG206.3041114E-446.01
GTCTCAC206.3041114E-446.021
CCTACTA206.3041114E-446.017
CACTGTT206.3041114E-446.020
CGCGACC253.4112003E-546.09
ACTTAGG253.4112003E-546.01
ACGGGCG206.3041114E-446.04
ACGGGAA950.046.04
TAGTTGG206.3041114E-446.01
AATGCGG501.6370905E-1146.01
CTAACGG253.4112003E-546.01
Produced by FastQC (version 0.11.5)